A mutant l-asparaginase II signal peptide improves the secretion of recombinant cyclodextrin glucanotransferase and the viability of escherichia coli
l-Asparaginase II signal peptide was used for the secretion of recombinant cyclodextrin glucanotransferase (CGTase) into the periplasmic space of E. coli. Despite its predominant localisation in the periplasm, CGTase activity was also detected in the extracellular medium, followed by cell lysis. Fiv...
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Springer Science+Business Media B.V.
2011
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| _version_ | 1796856407133257728 |
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| author | Ismail, Noor Faizah Hamdan, Salehhuddin Mahadi, Nor Muhammad Abdul Murad, Abdul Munir Rabu, Amir Abu Bakar, Farah Diba Klappa, Peter Md. Illias, Rosli |
| author_facet | Ismail, Noor Faizah Hamdan, Salehhuddin Mahadi, Nor Muhammad Abdul Murad, Abdul Munir Rabu, Amir Abu Bakar, Farah Diba Klappa, Peter Md. Illias, Rosli |
| author_sort | Ismail, Noor Faizah |
| collection | ePrints |
| description | l-Asparaginase II signal peptide was used for the secretion of recombinant cyclodextrin glucanotransferase (CGTase) into the periplasmic space of E. coli. Despite its predominant localisation in the periplasm, CGTase activity was also detected in the extracellular medium, followed by cell lysis. Five mutant signal peptides were constructed to improve the periplasmic levels of CGTase. N1R3 is a mutated signal peptide with the number of positively charged amino acid residues in the n-region increased to a net charge of +5. This mutant peptide produced a 1.7-fold enhancement of CGTase activity in the periplasm and significantly decreased cell lysis to 7.8% of the wild-type level. The formation of intracellular inclusion bodies was also reduced when this mutated signal peptide was used as judged by SDS-PAGE. Therefore, these results provide evidence of a cost-effective means of expression of recombinant proteins in E. coli. |
| first_indexed | 2024-03-05T18:42:29Z |
| format | Article |
| id | utm.eprints-28580 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T18:42:29Z |
| publishDate | 2011 |
| publisher | Springer Science+Business Media B.V. |
| record_format | dspace |
| spelling | utm.eprints-285802019-10-31T10:10:06Z http://eprints.utm.my/28580/ A mutant l-asparaginase II signal peptide improves the secretion of recombinant cyclodextrin glucanotransferase and the viability of escherichia coli Ismail, Noor Faizah Hamdan, Salehhuddin Mahadi, Nor Muhammad Abdul Murad, Abdul Munir Rabu, Amir Abu Bakar, Farah Diba Klappa, Peter Md. Illias, Rosli QH301 Biology l-Asparaginase II signal peptide was used for the secretion of recombinant cyclodextrin glucanotransferase (CGTase) into the periplasmic space of E. coli. Despite its predominant localisation in the periplasm, CGTase activity was also detected in the extracellular medium, followed by cell lysis. Five mutant signal peptides were constructed to improve the periplasmic levels of CGTase. N1R3 is a mutated signal peptide with the number of positively charged amino acid residues in the n-region increased to a net charge of +5. This mutant peptide produced a 1.7-fold enhancement of CGTase activity in the periplasm and significantly decreased cell lysis to 7.8% of the wild-type level. The formation of intracellular inclusion bodies was also reduced when this mutated signal peptide was used as judged by SDS-PAGE. Therefore, these results provide evidence of a cost-effective means of expression of recombinant proteins in E. coli. Springer Science+Business Media B.V. 2011-05 Article PeerReviewed Ismail, Noor Faizah and Hamdan, Salehhuddin and Mahadi, Nor Muhammad and Abdul Murad, Abdul Munir and Rabu, Amir and Abu Bakar, Farah Diba and Klappa, Peter and Md. Illias, Rosli (2011) A mutant l-asparaginase II signal peptide improves the secretion of recombinant cyclodextrin glucanotransferase and the viability of escherichia coli. Biotechnology Letters, 33 (5). pp. 999-1005. ISSN 1573-6776 http://dx.doi.org/10.1007/s10529-011-0517-8 DOI:0.1007/s10529-011-0517-8 |
| spellingShingle | QH301 Biology Ismail, Noor Faizah Hamdan, Salehhuddin Mahadi, Nor Muhammad Abdul Murad, Abdul Munir Rabu, Amir Abu Bakar, Farah Diba Klappa, Peter Md. Illias, Rosli A mutant l-asparaginase II signal peptide improves the secretion of recombinant cyclodextrin glucanotransferase and the viability of escherichia coli |
| title | A mutant l-asparaginase II signal peptide improves the secretion of recombinant cyclodextrin glucanotransferase and the viability of escherichia coli |
| title_full | A mutant l-asparaginase II signal peptide improves the secretion of recombinant cyclodextrin glucanotransferase and the viability of escherichia coli |
| title_fullStr | A mutant l-asparaginase II signal peptide improves the secretion of recombinant cyclodextrin glucanotransferase and the viability of escherichia coli |
| title_full_unstemmed | A mutant l-asparaginase II signal peptide improves the secretion of recombinant cyclodextrin glucanotransferase and the viability of escherichia coli |
| title_short | A mutant l-asparaginase II signal peptide improves the secretion of recombinant cyclodextrin glucanotransferase and the viability of escherichia coli |
| title_sort | mutant l asparaginase ii signal peptide improves the secretion of recombinant cyclodextrin glucanotransferase and the viability of escherichia coli |
| topic | QH301 Biology |
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