A mutant l-asparaginase II signal peptide improves the secretion of recombinant cyclodextrin glucanotransferase and the viability of escherichia coli
l-Asparaginase II signal peptide was used for the secretion of recombinant cyclodextrin glucanotransferase (CGTase) into the periplasmic space of E. coli. Despite its predominant localisation in the periplasm, CGTase activity was also detected in the extracellular medium, followed by cell lysis. Fiv...
| Main Authors: | , , , , , , , |
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| Format: | Article |
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Springer Science+Business Media B.V.
2011
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| Subjects: |