Isolation and characterization of a novel bacterium from the marine environment for trichloroacetic acid bioremediation
Halogenated compounds are an important class of environmental pollutants that are widely used in industrial chemicals such as solvents, herbicides, and pesticides. Many studies have been carried out to explore the biodegradation of these chemicals. Trichloroacetic acid (TCA) is one of the main halog...
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| Format: | Article |
| Language: | English |
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MDPI
2020
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| Online Access: | http://eprints.utm.my/28852/1/MahshidHeidarrezaei2020_IsolationandCharacterizationofaNovelBacterium.pdf |
| _version_ | 1796856452832296960 |
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| author | Heidarrezaei, Mahshid Shokravi, Hoofar Huyop, Fahrul Koloor, Seyed Saeid Rahimian Petru, Michal |
| author_facet | Heidarrezaei, Mahshid Shokravi, Hoofar Huyop, Fahrul Koloor, Seyed Saeid Rahimian Petru, Michal |
| author_sort | Heidarrezaei, Mahshid |
| collection | ePrints |
| description | Halogenated compounds are an important class of environmental pollutants that are widely used in industrial chemicals such as solvents, herbicides, and pesticides. Many studies have been carried out to explore the biodegradation of these chemicals. Trichloroacetic acid (TCA) is one of the main halogenated compounds that are carcinogenic to humans and animals. The bacterium was isolated fromthe northern coastline of Johor Strait. In this study, the ability of strainMH2to biodegrade TCA was evaluated by a growth experiment and dehalogenase enzyme assay. The growth profile of the isolated strain was examined. The doubling time for L. boronitolerans MH2 was found to be 32 h. The release of chloride ion in the degradation process was measured at 0.33 x 10-3 ± 0.03 molL-1 after 96 h when the growth curve had reached its maximum within the late bacterial exponential phase. The results showed that the strain had a promising ability to degrade TCA by producing dehalogenase enzyme when cell-free extracts were prepared from growth on TCA as the sole carbon source with enzyme-specific activity, 1.1 ± 0.05 μmolCl-min-1 mg-1 protein. Furthermore, the morphological, and biochemical aspects of the isolated bacterium were studied to identify and characterize the strain. The morphological observation of the isolated bacterium was seen to be a rod-shaped, Gram-positive, motile, heterotrophic, and spore-forming bacterium. The amplification of the 16S rRNA and gene analysis results indicated that the isolated bacterium had 98% similarity to Lysinibacillus boronitolerans. The morphological and biochemical tests supported the 16S rRNA gene amplification. To the best of the authors' knowledge, this is the first reported case of this genus of bacteria to degrade this type of halogenated compound. |
| first_indexed | 2024-03-05T18:43:10Z |
| format | Article |
| id | utm.eprints-28852 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T18:43:10Z |
| publishDate | 2020 |
| publisher | MDPI |
| record_format | dspace |
| spelling | utm.eprints-288522022-01-31T08:38:00Z http://eprints.utm.my/28852/ Isolation and characterization of a novel bacterium from the marine environment for trichloroacetic acid bioremediation Heidarrezaei, Mahshid Shokravi, Hoofar Huyop, Fahrul Koloor, Seyed Saeid Rahimian Petru, Michal TP Chemical technology Halogenated compounds are an important class of environmental pollutants that are widely used in industrial chemicals such as solvents, herbicides, and pesticides. Many studies have been carried out to explore the biodegradation of these chemicals. Trichloroacetic acid (TCA) is one of the main halogenated compounds that are carcinogenic to humans and animals. The bacterium was isolated fromthe northern coastline of Johor Strait. In this study, the ability of strainMH2to biodegrade TCA was evaluated by a growth experiment and dehalogenase enzyme assay. The growth profile of the isolated strain was examined. The doubling time for L. boronitolerans MH2 was found to be 32 h. The release of chloride ion in the degradation process was measured at 0.33 x 10-3 ± 0.03 molL-1 after 96 h when the growth curve had reached its maximum within the late bacterial exponential phase. The results showed that the strain had a promising ability to degrade TCA by producing dehalogenase enzyme when cell-free extracts were prepared from growth on TCA as the sole carbon source with enzyme-specific activity, 1.1 ± 0.05 μmolCl-min-1 mg-1 protein. Furthermore, the morphological, and biochemical aspects of the isolated bacterium were studied to identify and characterize the strain. The morphological observation of the isolated bacterium was seen to be a rod-shaped, Gram-positive, motile, heterotrophic, and spore-forming bacterium. The amplification of the 16S rRNA and gene analysis results indicated that the isolated bacterium had 98% similarity to Lysinibacillus boronitolerans. The morphological and biochemical tests supported the 16S rRNA gene amplification. To the best of the authors' knowledge, this is the first reported case of this genus of bacteria to degrade this type of halogenated compound. MDPI 2020-07-01 Article PeerReviewed application/pdf en http://eprints.utm.my/28852/1/MahshidHeidarrezaei2020_IsolationandCharacterizationofaNovelBacterium.pdf Heidarrezaei, Mahshid and Shokravi, Hoofar and Huyop, Fahrul and Koloor, Seyed Saeid Rahimian and Petru, Michal (2020) Isolation and characterization of a novel bacterium from the marine environment for trichloroacetic acid bioremediation. Applied Sciences (Switzerland), 10 (13). pp. 1-17. ISSN 2076-3417 http://dx.doi.org/10.3390/app10134593 DOI:10.3390/app10134593 |
| spellingShingle | TP Chemical technology Heidarrezaei, Mahshid Shokravi, Hoofar Huyop, Fahrul Koloor, Seyed Saeid Rahimian Petru, Michal Isolation and characterization of a novel bacterium from the marine environment for trichloroacetic acid bioremediation |
| title | Isolation and characterization of a novel bacterium from the marine environment for trichloroacetic acid bioremediation |
| title_full | Isolation and characterization of a novel bacterium from the marine environment for trichloroacetic acid bioremediation |
| title_fullStr | Isolation and characterization of a novel bacterium from the marine environment for trichloroacetic acid bioremediation |
| title_full_unstemmed | Isolation and characterization of a novel bacterium from the marine environment for trichloroacetic acid bioremediation |
| title_short | Isolation and characterization of a novel bacterium from the marine environment for trichloroacetic acid bioremediation |
| title_sort | isolation and characterization of a novel bacterium from the marine environment for trichloroacetic acid bioremediation |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/28852/1/MahshidHeidarrezaei2020_IsolationandCharacterizationofaNovelBacterium.pdf |
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