Flavonoids and phenolic acids from Labisia pumila (Kacip Fatimah)

Both total phenolic content (TPC) and total flavonoid content (TFC) of Labisia pumila extracts were determined spectrophotometrically. L. pumila leaves extracted in 60% methanol (MeOH) were fractionated on C18 cartridge and the antioxidant property of each fraction was determined by measuring free r...

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Main Authors: Lee, Suan Chua, Abdul Latiff, Norliza, Sze, Yean Lee, Chew, Tin Lee, Sarmidi, Mohamad Roji, Abdul Aziz, Ramlan
Format: Article
Published: Elsevier Ltd. 2011
Subjects:
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author Lee, Suan Chua
Abdul Latiff, Norliza
Sze, Yean Lee
Chew, Tin Lee
Sarmidi, Mohamad Roji
Abdul Aziz, Ramlan
author_facet Lee, Suan Chua
Abdul Latiff, Norliza
Sze, Yean Lee
Chew, Tin Lee
Sarmidi, Mohamad Roji
Abdul Aziz, Ramlan
author_sort Lee, Suan Chua
collection ePrints
description Both total phenolic content (TPC) and total flavonoid content (TFC) of Labisia pumila extracts were determined spectrophotometrically. L. pumila leaves extracted in 60% methanol (MeOH) were fractionated on C18 cartridge and the antioxidant property of each fraction was determined by measuring free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity. The 40% MeOH fraction exhibited the highest scavenging activity. Nine flavonols (quercetin, myricetin and kaempferol), two flavanols (catechin and epigallocatechin) and nine phenolic acids were identified from this active fraction by UPLC–ESI-MS/MS, and confirmed by comparison with the mass spectra of standard aglycones, theoretical fragments generated from MS Fragmenter software, and literature values.
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spelling utm.eprints-296872019-04-25T01:18:27Z http://eprints.utm.my/29687/ Flavonoids and phenolic acids from Labisia pumila (Kacip Fatimah) Lee, Suan Chua Abdul Latiff, Norliza Sze, Yean Lee Chew, Tin Lee Sarmidi, Mohamad Roji Abdul Aziz, Ramlan QD Chemistry Both total phenolic content (TPC) and total flavonoid content (TFC) of Labisia pumila extracts were determined spectrophotometrically. L. pumila leaves extracted in 60% methanol (MeOH) were fractionated on C18 cartridge and the antioxidant property of each fraction was determined by measuring free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity. The 40% MeOH fraction exhibited the highest scavenging activity. Nine flavonols (quercetin, myricetin and kaempferol), two flavanols (catechin and epigallocatechin) and nine phenolic acids were identified from this active fraction by UPLC–ESI-MS/MS, and confirmed by comparison with the mass spectra of standard aglycones, theoretical fragments generated from MS Fragmenter software, and literature values. Elsevier Ltd. 2011-08-01 Article PeerReviewed Lee, Suan Chua and Abdul Latiff, Norliza and Sze, Yean Lee and Chew, Tin Lee and Sarmidi, Mohamad Roji and Abdul Aziz, Ramlan (2011) Flavonoids and phenolic acids from Labisia pumila (Kacip Fatimah). Food Chemistry, 127 (3). pp. 1186-1192. ISSN 0308-8146 http://dx.doi.org/10.1016/j.foodchem.2011.01.122 DOI:10.1016/j.foodchem.2011.01.122
spellingShingle QD Chemistry
Lee, Suan Chua
Abdul Latiff, Norliza
Sze, Yean Lee
Chew, Tin Lee
Sarmidi, Mohamad Roji
Abdul Aziz, Ramlan
Flavonoids and phenolic acids from Labisia pumila (Kacip Fatimah)
title Flavonoids and phenolic acids from Labisia pumila (Kacip Fatimah)
title_full Flavonoids and phenolic acids from Labisia pumila (Kacip Fatimah)
title_fullStr Flavonoids and phenolic acids from Labisia pumila (Kacip Fatimah)
title_full_unstemmed Flavonoids and phenolic acids from Labisia pumila (Kacip Fatimah)
title_short Flavonoids and phenolic acids from Labisia pumila (Kacip Fatimah)
title_sort flavonoids and phenolic acids from labisia pumila kacip fatimah
topic QD Chemistry
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