In silico analyses of flavin reductase from citrobacter freundii A1
Objective: The aim of the present study was to describe the structural and phylogenetic features of flavin reductase from C.freundii Al using bioinfonnatics tools. Materials and methods:The flavin reductase (Ire) gene from a dye-degrading bacterium, C. freundii Al was isolated and amplified by Polym...
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| Format: | Article |
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Insight Knowledge Ltd
2012
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| _version_ | 1796856761503711232 |
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| author | Giek, Far Chan Mohd. Yusoff, Abdull Rahim Abdul Rashid, Noor Aini |
| author_facet | Giek, Far Chan Mohd. Yusoff, Abdull Rahim Abdul Rashid, Noor Aini |
| author_sort | Giek, Far Chan |
| collection | ePrints |
| description | Objective: The aim of the present study was to describe the structural and phylogenetic features of flavin reductase from C.freundii Al using bioinfonnatics tools. Materials and methods:The flavin reductase (Ire) gene from a dye-degrading bacterium, C. freundii Al was isolated and amplified by Polymerase Chain Reaction (peR). The gene encodes for NAD(P)H:flavin oxidoreductase, an enzyme that catalyzes the reduction of soluble flavins by reduced pyridine nucleotides this was believed to be the azoreductase from C. freundii AI. The gene, approximately 0.8 kb was sequenced and in silica analyses of the nucleotide sequence were performed. Results: From phlyogenetic analyses, we observed that flavin reductase enzyme generally existed in most microorganisms and the enzyme from C.freundii Al is conserved among the Gram-negative bacteria. The protein function of the flavin reductase coded was predicted based on the motifs of deduced amino acid sequence. The amino acid sequence of flavin reductase from C. freundii Al was compared with other azoreductases and was found to be a unique NADPH-preferred azoreductase. Conclusion: Hence, in silica characterization of flavin reductase gene presented various features of the gene and this would facilitate molecular studies of the gene and reveal the functional role of the enzyme. |
| first_indexed | 2024-03-05T18:47:56Z |
| format | Article |
| id | utm.eprints-30539 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T18:47:56Z |
| publishDate | 2012 |
| publisher | Insight Knowledge Ltd |
| record_format | dspace |
| spelling | utm.eprints-305392019-09-24T09:36:07Z http://eprints.utm.my/30539/ In silico analyses of flavin reductase from citrobacter freundii A1 Giek, Far Chan Mohd. Yusoff, Abdull Rahim Abdul Rashid, Noor Aini Q Science (General) Objective: The aim of the present study was to describe the structural and phylogenetic features of flavin reductase from C.freundii Al using bioinfonnatics tools. Materials and methods:The flavin reductase (Ire) gene from a dye-degrading bacterium, C. freundii Al was isolated and amplified by Polymerase Chain Reaction (peR). The gene encodes for NAD(P)H:flavin oxidoreductase, an enzyme that catalyzes the reduction of soluble flavins by reduced pyridine nucleotides this was believed to be the azoreductase from C. freundii AI. The gene, approximately 0.8 kb was sequenced and in silica analyses of the nucleotide sequence were performed. Results: From phlyogenetic analyses, we observed that flavin reductase enzyme generally existed in most microorganisms and the enzyme from C.freundii Al is conserved among the Gram-negative bacteria. The protein function of the flavin reductase coded was predicted based on the motifs of deduced amino acid sequence. The amino acid sequence of flavin reductase from C. freundii Al was compared with other azoreductases and was found to be a unique NADPH-preferred azoreductase. Conclusion: Hence, in silica characterization of flavin reductase gene presented various features of the gene and this would facilitate molecular studies of the gene and reveal the functional role of the enzyme. Insight Knowledge Ltd 2012 Article PeerReviewed Giek, Far Chan and Mohd. Yusoff, Abdull Rahim and Abdul Rashid, Noor Aini (2012) In silico analyses of flavin reductase from citrobacter freundii A1. Insight Bioinformatics, 2 (1). pp. 1-8. ISSN 2223-9553 http://dx.doi.org/10.5567/BIOINFO-IK.2012.1.8 DOl:10.5567/BIOINFO-IK.2012.1.8 |
| spellingShingle | Q Science (General) Giek, Far Chan Mohd. Yusoff, Abdull Rahim Abdul Rashid, Noor Aini In silico analyses of flavin reductase from citrobacter freundii A1 |
| title | In silico analyses of flavin reductase from citrobacter freundii A1 |
| title_full | In silico analyses of flavin reductase from citrobacter freundii A1 |
| title_fullStr | In silico analyses of flavin reductase from citrobacter freundii A1 |
| title_full_unstemmed | In silico analyses of flavin reductase from citrobacter freundii A1 |
| title_short | In silico analyses of flavin reductase from citrobacter freundii A1 |
| title_sort | in silico analyses of flavin reductase from citrobacter freundii a1 |
| topic | Q Science (General) |
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