Development of Unsupported Composite Alumina Sol/Poly(vinyl alcohol) Membranes For Glucose Oxidase Immobilization
An important aspect of biosensor developments is the immobilization method of the sensing agent within the solid matrix. In the continuous search for a suitable support material for glucose oxidase, a composite membrane of alumina sol(AlOOH)/poly(vinyl alcohol)(PVA) was investigated. The alumina sol...
| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English |
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2005
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| Online Access: | http://eprints.utm.my/3061/1/MTE-13.doc |
| _version_ | 1825909336386306048 |
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| author | Wong, Fui Ling Abdul Aziz, Azila |
| author_facet | Wong, Fui Ling Abdul Aziz, Azila |
| author_sort | Wong, Fui Ling |
| collection | ePrints |
| description | An important aspect of biosensor developments is the immobilization method of the sensing agent within the solid matrix. In the continuous search for a suitable support material for glucose oxidase, a composite membrane of alumina sol(AlOOH)/poly(vinyl alcohol)(PVA) was investigated. The alumina sol was prepared using aluminium iso-propoxide via the sol-gel process. PVA was employed as the organic binder to enhance the mechanical strength of the porous sol-gel membranes. The ability of the composite membrane in retaining glucose oxidase and the apparent enzyme activities of the composite membranes were studied. The resulting composite membranes were found to be crack-free, stable and still very active after 60 days. However, the enzyme leakage period was observed to be quite long. The enzyme was still leaking from the membrane after more than 400 hours albeit at a very low level. |
| first_indexed | 2024-03-05T18:00:37Z |
| format | Conference or Workshop Item |
| id | utm.eprints-3061 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T18:00:37Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | utm.eprints-30612017-08-27T04:58:45Z http://eprints.utm.my/3061/ Development of Unsupported Composite Alumina Sol/Poly(vinyl alcohol) Membranes For Glucose Oxidase Immobilization Wong, Fui Ling Abdul Aziz, Azila QH301 Biology An important aspect of biosensor developments is the immobilization method of the sensing agent within the solid matrix. In the continuous search for a suitable support material for glucose oxidase, a composite membrane of alumina sol(AlOOH)/poly(vinyl alcohol)(PVA) was investigated. The alumina sol was prepared using aluminium iso-propoxide via the sol-gel process. PVA was employed as the organic binder to enhance the mechanical strength of the porous sol-gel membranes. The ability of the composite membrane in retaining glucose oxidase and the apparent enzyme activities of the composite membranes were studied. The resulting composite membranes were found to be crack-free, stable and still very active after 60 days. However, the enzyme leakage period was observed to be quite long. The enzyme was still leaking from the membrane after more than 400 hours albeit at a very low level. 2005-12-08 Conference or Workshop Item PeerReviewed application/msword en http://eprints.utm.my/3061/1/MTE-13.doc Wong, Fui Ling and Abdul Aziz, Azila (2005) Development of Unsupported Composite Alumina Sol/Poly(vinyl alcohol) Membranes For Glucose Oxidase Immobilization. In: 2nd International Conference on Chemical and Bioprocess Engineering in conjuction with 19th Symposium of Malaysian Chemical Engineers (SOMChE 2005), 8-10 December 2005, Kota Kinabalu, Sabah. |
| spellingShingle | QH301 Biology Wong, Fui Ling Abdul Aziz, Azila Development of Unsupported Composite Alumina Sol/Poly(vinyl alcohol) Membranes For Glucose Oxidase Immobilization |
| title | Development of Unsupported Composite Alumina Sol/Poly(vinyl alcohol) Membranes For Glucose Oxidase Immobilization |
| title_full | Development of Unsupported Composite Alumina Sol/Poly(vinyl alcohol) Membranes For Glucose Oxidase Immobilization |
| title_fullStr | Development of Unsupported Composite Alumina Sol/Poly(vinyl alcohol) Membranes For Glucose Oxidase Immobilization |
| title_full_unstemmed | Development of Unsupported Composite Alumina Sol/Poly(vinyl alcohol) Membranes For Glucose Oxidase Immobilization |
| title_short | Development of Unsupported Composite Alumina Sol/Poly(vinyl alcohol) Membranes For Glucose Oxidase Immobilization |
| title_sort | development of unsupported composite alumina sol poly vinyl alcohol membranes for glucose oxidase immobilization |
| topic | QH301 Biology |
| url | http://eprints.utm.my/3061/1/MTE-13.doc |
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