Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate
In this study, the effect of pulsed-low intensity ultrasound on cell proliferation, collagen production and glycosaminoglycan deposition by 3T3 fibroblasts encapsulated in alginate was evaluated. Hoechst 33258 assay for cell number, hydroxyproline assay for collagen content and dimethylamine blue as...
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| Format: | Article |
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SAGE Publications Inc.
2012
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| author | Mohd. Bohari, Siti Pauliena Liam, M. Grover Hukins, David W. L. |
| author_facet | Mohd. Bohari, Siti Pauliena Liam, M. Grover Hukins, David W. L. |
| author_sort | Mohd. Bohari, Siti Pauliena |
| collection | ePrints |
| description | In this study, the effect of pulsed-low intensity ultrasound on cell proliferation, collagen production and glycosaminoglycan deposition by 3T3 fibroblasts encapsulated in alginate was evaluated. Hoechst 33258 assay for cell number, hydroxyproline assay for collagen content and dimethylamine blue assay for glycosaminoglycan content were performed on samples from cell cultures treated with pulsed-low intensity ultrasound and a control group. Pulsed-low intensity ultrasound shows no effect on cell proliferation, while collagen and glycosaminoglycan contents were consistently higher in the samples treated with pulsed-low intensity ultrasound, showing a statistically significant difference (p < 0.05) on day 10. Alcian blue staining showed that glycosaminoglycans were deposited around the cells in both groups. These results suggest that pulsed-low intensity ultrasound shows no effect on cell proliferation but has potential for inducing collagen and glycosaminoglycan production in cells cultured in alginate gels. |
| first_indexed | 2024-03-05T18:48:53Z |
| format | Article |
| id | utm.eprints-31127 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T18:48:53Z |
| publishDate | 2012 |
| publisher | SAGE Publications Inc. |
| record_format | dspace |
| spelling | utm.eprints-311272018-10-14T07:23:51Z http://eprints.utm.my/31127/ Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate Mohd. Bohari, Siti Pauliena Liam, M. Grover Hukins, David W. L. Q Science In this study, the effect of pulsed-low intensity ultrasound on cell proliferation, collagen production and glycosaminoglycan deposition by 3T3 fibroblasts encapsulated in alginate was evaluated. Hoechst 33258 assay for cell number, hydroxyproline assay for collagen content and dimethylamine blue assay for glycosaminoglycan content were performed on samples from cell cultures treated with pulsed-low intensity ultrasound and a control group. Pulsed-low intensity ultrasound shows no effect on cell proliferation, while collagen and glycosaminoglycan contents were consistently higher in the samples treated with pulsed-low intensity ultrasound, showing a statistically significant difference (p < 0.05) on day 10. Alcian blue staining showed that glycosaminoglycans were deposited around the cells in both groups. These results suggest that pulsed-low intensity ultrasound shows no effect on cell proliferation but has potential for inducing collagen and glycosaminoglycan production in cells cultured in alginate gels. SAGE Publications Inc. 2012 Article PeerReviewed Mohd. Bohari, Siti Pauliena and Liam, M. Grover and Hukins, David W. L. (2012) Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate. Journal of Tissue Engineering, 3 (1). pp. 1-7. ISSN 2041-7314 http://journals.sagepub.com/doi/10.1177/2041731412454672 DOI:10.1177/2041731412454672 |
| spellingShingle | Q Science Mohd. Bohari, Siti Pauliena Liam, M. Grover Hukins, David W. L. Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate |
| title | Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate |
| title_full | Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate |
| title_fullStr | Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate |
| title_full_unstemmed | Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate |
| title_short | Pulsed-low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate |
| title_sort | pulsed low intensity ultrasound enhances extracellular matrix production by fibroblasts encapsulated in alginate |
| topic | Q Science |
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