Cloning and functional analysis of the genes coding for 4-Aminobenzenesulfonate 3,4-dioxygenase from hydrogenophaga Sp Pbc
The gene coding for the oxygenase component, sadA, of 4-aminobenzenesulfonate (4-ABS) 3,4-dioxygenase in Hydrogenophaga sp. PBC was previously identified via transposon mutagenesis. Expression of wild-type sadA in trans restored the ability of the sadA mutant to grow on 4-ABS. The inclusion of sadB...
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Society for General Microbiology
2012
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| author | Han, Ming Gan Shahir, Shafinaz Yahya, Adibah |
| author_facet | Han, Ming Gan Shahir, Shafinaz Yahya, Adibah |
| author_sort | Han, Ming Gan |
| collection | ePrints |
| description | The gene coding for the oxygenase component, sadA, of 4-aminobenzenesulfonate (4-ABS) 3,4-dioxygenase in Hydrogenophaga sp. PBC was previously identified via transposon mutagenesis. Expression of wild-type sadA in trans restored the ability of the sadA mutant to grow on 4-ABS. The inclusion of sadB and sadD, coding for a putative glutamine-synthetase-like protein and a plant-type ferredoxin, respectively, further improved the efficiency of 4-ABS degradation. Transcription analysis using the gfp promoter probe plasmid showed that sadABD was expressed during growth on 4-ABS and 4-sulfocatechol. Heterologous expression of sadABD in Escherichia coli led to the biotransformation of 4-ABS to a metabolite which shared a similar retention time and UV/vis profile with 4-sulfocatechol. The putative reductase gene sadC was isolated via degenerate PCR and expression of sadC and sadABD in E. coli led to maximal 4-ABS biotransformation. In E. coli, the deletion of sadB completely eliminated dioxygenase activity while the deletion of sadC or sadD led to a decrease in dioxygenase activity. Phylogenetic analysis of SadB showed that it is closely related to the glutamine-synthetase-like proteins involved in the aniline degradation pathway. This is the first discovery, to our knowledge, of the functional genetic components for 4-ABS aromatic ring hydroxylation in the bacterial domain. |
| first_indexed | 2024-03-05T18:52:45Z |
| format | Article |
| id | utm.eprints-33005 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T18:52:45Z |
| publishDate | 2012 |
| publisher | Society for General Microbiology |
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| spelling | utm.eprints-330052019-03-05T02:03:42Z http://eprints.utm.my/33005/ Cloning and functional analysis of the genes coding for 4-Aminobenzenesulfonate 3,4-dioxygenase from hydrogenophaga Sp Pbc Han, Ming Gan Shahir, Shafinaz Yahya, Adibah Q Science The gene coding for the oxygenase component, sadA, of 4-aminobenzenesulfonate (4-ABS) 3,4-dioxygenase in Hydrogenophaga sp. PBC was previously identified via transposon mutagenesis. Expression of wild-type sadA in trans restored the ability of the sadA mutant to grow on 4-ABS. The inclusion of sadB and sadD, coding for a putative glutamine-synthetase-like protein and a plant-type ferredoxin, respectively, further improved the efficiency of 4-ABS degradation. Transcription analysis using the gfp promoter probe plasmid showed that sadABD was expressed during growth on 4-ABS and 4-sulfocatechol. Heterologous expression of sadABD in Escherichia coli led to the biotransformation of 4-ABS to a metabolite which shared a similar retention time and UV/vis profile with 4-sulfocatechol. The putative reductase gene sadC was isolated via degenerate PCR and expression of sadC and sadABD in E. coli led to maximal 4-ABS biotransformation. In E. coli, the deletion of sadB completely eliminated dioxygenase activity while the deletion of sadC or sadD led to a decrease in dioxygenase activity. Phylogenetic analysis of SadB showed that it is closely related to the glutamine-synthetase-like proteins involved in the aniline degradation pathway. This is the first discovery, to our knowledge, of the functional genetic components for 4-ABS aromatic ring hydroxylation in the bacterial domain. Society for General Microbiology 2012-08 Article PeerReviewed Han, Ming Gan and Shahir, Shafinaz and Yahya, Adibah (2012) Cloning and functional analysis of the genes coding for 4-Aminobenzenesulfonate 3,4-dioxygenase from hydrogenophaga Sp Pbc. Microbiology-Sgm, 158 (Pt8). pp. 1933-1941. ISSN 1350-0872 http://dx.doi.org/10.1099/mic.0.059550-0 DOI:10.1099/mic.0.059550-0 |
| spellingShingle | Q Science Han, Ming Gan Shahir, Shafinaz Yahya, Adibah Cloning and functional analysis of the genes coding for 4-Aminobenzenesulfonate 3,4-dioxygenase from hydrogenophaga Sp Pbc |
| title | Cloning and functional analysis of the genes coding for 4-Aminobenzenesulfonate 3,4-dioxygenase from hydrogenophaga Sp Pbc |
| title_full | Cloning and functional analysis of the genes coding for 4-Aminobenzenesulfonate 3,4-dioxygenase from hydrogenophaga Sp Pbc |
| title_fullStr | Cloning and functional analysis of the genes coding for 4-Aminobenzenesulfonate 3,4-dioxygenase from hydrogenophaga Sp Pbc |
| title_full_unstemmed | Cloning and functional analysis of the genes coding for 4-Aminobenzenesulfonate 3,4-dioxygenase from hydrogenophaga Sp Pbc |
| title_short | Cloning and functional analysis of the genes coding for 4-Aminobenzenesulfonate 3,4-dioxygenase from hydrogenophaga Sp Pbc |
| title_sort | cloning and functional analysis of the genes coding for 4 aminobenzenesulfonate 3 4 dioxygenase from hydrogenophaga sp pbc |
| topic | Q Science |
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