Single cell trapping in microfluidic channel via hydrodynamic manipulation
Microfluidic devices are important for the single cell analysis such as cell mechanical and electrical characterization. Single cell characterization could be related to many significant applications including early disease diagnosis. However to perform the single cell manipulation, firstly a single...
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| Format: | Conference or Workshop Item |
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2013
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| _version_ | 1796857742388887552 |
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| author | Ahmad Khalili, Amelia Mohd. Basri, Mohd. Ariffanan Ahmad, Mohd. Ridzuan |
| author_facet | Ahmad Khalili, Amelia Mohd. Basri, Mohd. Ariffanan Ahmad, Mohd. Ridzuan |
| author_sort | Ahmad Khalili, Amelia |
| collection | ePrints |
| description | Microfluidic devices are important for the single cell analysis such as cell mechanical and electrical characterization. Single cell characterization could be related to many significant applications including early disease diagnosis. However to perform the single cell manipulation, firstly a single cell have to be isolated and a platform for the cell manipulation have to be provided. One of the methods to trap a single cell is by using hydrodynamic trapping in the microfluidic channel. This study provides a finite element model for single cell trapping for a yeast cell model. The objectives of the simulations are to obtain the appropriate channels’ geometry and optimized ratio of the fluid’s inlet and suction flow rate to trap a single yeast cell. Trap channel was designed to trap a 5 µm yeast cell with a suction hole placed in the end of the trap channel. Design geometry and ratio of fluid flow rates referring to the hydrodynamic concept were studied for the cell trapping model. The analysis was carried out using numerical solutions from the finite element ABAQUS-FEA software. Using the cell trapping model, a single yeast cell able to be trapped into the trap channel with optimized channel’s suction hole’s geometry and appropriate fluid’s inlet and suction flow rate ratio. |
| first_indexed | 2024-03-05T19:02:21Z |
| format | Conference or Workshop Item |
| id | utm.eprints-38676 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:02:21Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | utm.eprints-386762017-09-26T07:08:36Z http://eprints.utm.my/38676/ Single cell trapping in microfluidic channel via hydrodynamic manipulation Ahmad Khalili, Amelia Mohd. Basri, Mohd. Ariffanan Ahmad, Mohd. Ridzuan TK Electrical engineering. Electronics Nuclear engineering Microfluidic devices are important for the single cell analysis such as cell mechanical and electrical characterization. Single cell characterization could be related to many significant applications including early disease diagnosis. However to perform the single cell manipulation, firstly a single cell have to be isolated and a platform for the cell manipulation have to be provided. One of the methods to trap a single cell is by using hydrodynamic trapping in the microfluidic channel. This study provides a finite element model for single cell trapping for a yeast cell model. The objectives of the simulations are to obtain the appropriate channels’ geometry and optimized ratio of the fluid’s inlet and suction flow rate to trap a single yeast cell. Trap channel was designed to trap a 5 µm yeast cell with a suction hole placed in the end of the trap channel. Design geometry and ratio of fluid flow rates referring to the hydrodynamic concept were studied for the cell trapping model. The analysis was carried out using numerical solutions from the finite element ABAQUS-FEA software. Using the cell trapping model, a single yeast cell able to be trapped into the trap channel with optimized channel’s suction hole’s geometry and appropriate fluid’s inlet and suction flow rate ratio. 2013 Conference or Workshop Item PeerReviewed Ahmad Khalili, Amelia and Mohd. Basri, Mohd. Ariffanan and Ahmad, Mohd. Ridzuan (2013) Single cell trapping in microfluidic channel via hydrodynamic manipulation. In: 2013 IEEE International Conference on Control System, Computing and Engineering, 2013, Melbourne, Australia. |
| spellingShingle | TK Electrical engineering. Electronics Nuclear engineering Ahmad Khalili, Amelia Mohd. Basri, Mohd. Ariffanan Ahmad, Mohd. Ridzuan Single cell trapping in microfluidic channel via hydrodynamic manipulation |
| title | Single cell trapping in microfluidic channel via hydrodynamic manipulation |
| title_full | Single cell trapping in microfluidic channel via hydrodynamic manipulation |
| title_fullStr | Single cell trapping in microfluidic channel via hydrodynamic manipulation |
| title_full_unstemmed | Single cell trapping in microfluidic channel via hydrodynamic manipulation |
| title_short | Single cell trapping in microfluidic channel via hydrodynamic manipulation |
| title_sort | single cell trapping in microfluidic channel via hydrodynamic manipulation |
| topic | TK Electrical engineering. Electronics Nuclear engineering |
| work_keys_str_mv | AT ahmadkhaliliamelia singlecelltrappinginmicrofluidicchannelviahydrodynamicmanipulation AT mohdbasrimohdariffanan singlecelltrappinginmicrofluidicchannelviahydrodynamicmanipulation AT ahmadmohdridzuan singlecelltrappinginmicrofluidicchannelviahydrodynamicmanipulation |