Characterization of a labrys sp. strain wy1 able to utilize 2,2-dichloropropiote (2,2-DCP) as sole source of carbon
Industrial chemicals and pesticides from agricultural activities cause a considerable threat to the environment. 2,2-dichloropropionate (2,2-DCP) is a synthetic halogenated compound used as herbicide. High concentration of 2,2-DCP is toxic if released to the environment and may pollute the soil and...
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| Format: | Article |
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Academic Journals
2011
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| _version_ | 1796858618141736960 |
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| author | Wen, Yong Wong Huyop, Fahrul |
| author_facet | Wen, Yong Wong Huyop, Fahrul |
| author_sort | Wen, Yong Wong |
| collection | ePrints |
| description | Industrial chemicals and pesticides from agricultural activities cause a considerable threat to the environment. 2,2-dichloropropionate (2,2-DCP) is a synthetic halogenated compound used as herbicide. High concentration of 2,2-DCP is toxic if released to the environment and may pollute the soil and ground water source. Using current enrichment technique it was expected to isolate a new bacteria species able to degrade a-halocarboxylic acid. Strain Wy1 isolated from soil in Melaka rubber estate was able to utilize 30 mM 2, 2-DCP as the sole source of carbon and energy with maximum chloride ion released of 0.27 mmol/L in the liquid growth medium. The biochemical test and 16S rRNA analysis suggested that the bacterial identity was from the genus Labrys sp., and therefore it was designated as Labrys sp. strain Wy1. The cells doubling time in 30 mM 2,2-DCP liquid minimal medium was 33.44 h. Cell growth was inhibited when grown in liquid minimal medium above 30 mM 2,2-DCP due to the toxicity of 2,2-DCP to the cells. This is the first reported case that the genus Labrys sp. is able to degrade 2,2-DCP. |
| first_indexed | 2024-03-05T19:15:08Z |
| format | Article |
| id | utm.eprints-44781 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:15:08Z |
| publishDate | 2011 |
| publisher | Academic Journals |
| record_format | dspace |
| spelling | utm.eprints-447812017-09-13T03:45:04Z http://eprints.utm.my/44781/ Characterization of a labrys sp. strain wy1 able to utilize 2,2-dichloropropiote (2,2-DCP) as sole source of carbon Wen, Yong Wong Huyop, Fahrul TP Chemical technology Industrial chemicals and pesticides from agricultural activities cause a considerable threat to the environment. 2,2-dichloropropionate (2,2-DCP) is a synthetic halogenated compound used as herbicide. High concentration of 2,2-DCP is toxic if released to the environment and may pollute the soil and ground water source. Using current enrichment technique it was expected to isolate a new bacteria species able to degrade a-halocarboxylic acid. Strain Wy1 isolated from soil in Melaka rubber estate was able to utilize 30 mM 2, 2-DCP as the sole source of carbon and energy with maximum chloride ion released of 0.27 mmol/L in the liquid growth medium. The biochemical test and 16S rRNA analysis suggested that the bacterial identity was from the genus Labrys sp., and therefore it was designated as Labrys sp. strain Wy1. The cells doubling time in 30 mM 2,2-DCP liquid minimal medium was 33.44 h. Cell growth was inhibited when grown in liquid minimal medium above 30 mM 2,2-DCP due to the toxicity of 2,2-DCP to the cells. This is the first reported case that the genus Labrys sp. is able to degrade 2,2-DCP. Academic Journals 2011 Article PeerReviewed Wen, Yong Wong and Huyop, Fahrul (2011) Characterization of a labrys sp. strain wy1 able to utilize 2,2-dichloropropiote (2,2-DCP) as sole source of carbon. African Journal of Microbiology Research, 5 (20). pp. 3282-3288. ISSN 1996-0808 |
| spellingShingle | TP Chemical technology Wen, Yong Wong Huyop, Fahrul Characterization of a labrys sp. strain wy1 able to utilize 2,2-dichloropropiote (2,2-DCP) as sole source of carbon |
| title | Characterization of a labrys sp. strain wy1 able to utilize 2,2-dichloropropiote (2,2-DCP) as sole source of carbon |
| title_full | Characterization of a labrys sp. strain wy1 able to utilize 2,2-dichloropropiote (2,2-DCP) as sole source of carbon |
| title_fullStr | Characterization of a labrys sp. strain wy1 able to utilize 2,2-dichloropropiote (2,2-DCP) as sole source of carbon |
| title_full_unstemmed | Characterization of a labrys sp. strain wy1 able to utilize 2,2-dichloropropiote (2,2-DCP) as sole source of carbon |
| title_short | Characterization of a labrys sp. strain wy1 able to utilize 2,2-dichloropropiote (2,2-DCP) as sole source of carbon |
| title_sort | characterization of a labrys sp strain wy1 able to utilize 2 2 dichloropropiote 2 2 dcp as sole source of carbon |
| topic | TP Chemical technology |
| work_keys_str_mv | AT wenyongwong characterizationofalabrysspstrainwy1abletoutilize22dichloropropiote22dcpassolesourceofcarbon AT huyopfahrul characterizationofalabrysspstrainwy1abletoutilize22dichloropropiote22dcpassolesourceofcarbon |