Identification of genes involved in the 4-aminobenzenesulfote degradation pathway of hyrogenophaga sp.PBC via transposon mutagenesis
Genes involved in the 4-aminobenzenesulfonate (4-ABS) degradation pathway of Hydrogenophaga sp. PBC were identified using transposon mutagenesis. The screening of 10 000 mutants for incomplete 4-ABS biotransformation identified four mutants with single transposon insertion. Genes with insertions tha...
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| Format: | Article |
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Oxford University Press
2011
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| author | Han, Ming Gan Ibrahim, Zaharah Shahir, Shafinaz Yahya, Adibah |
| author_facet | Han, Ming Gan Ibrahim, Zaharah Shahir, Shafinaz Yahya, Adibah |
| author_sort | Han, Ming Gan |
| collection | ePrints |
| description | Genes involved in the 4-aminobenzenesulfonate (4-ABS) degradation pathway of Hydrogenophaga sp. PBC were identified using transposon mutagenesis. The screening of 10 000 mutants for incomplete 4-ABS biotransformation identified four mutants with single transposon insertion. Genes with insertions that impaired the ability to utilize 4-ABS for growth included (1) 4-sulfocatechol 1,2-dioxygenase b-subunit (pcaH2) and 3-sulfomuconate cycloisomerase involved in the modified b-ketoadipate pathway; (2) 4-aminobenzenesulfonate 3,4-dioxy- genase component (sadA) involved in aromatic ring hydroxylation; and (3) transposase gene homolog with a putative cis-diol dehydrogenase gene located downstream. The pcaH2 mutant strain accumulated brown metabolite during growth on 4-ABS which was identified as 4-sulfocatechol through thin layer chromatography and HPLC analyses. Supplementation of w ild-type sadA gene in trans restored the 4-ABS degradation ability of the sadA mutant, thus supporting the annotation of its disrupted gene. |
| first_indexed | 2024-03-05T19:15:41Z |
| format | Article |
| id | utm.eprints-44970 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:15:41Z |
| publishDate | 2011 |
| publisher | Oxford University Press |
| record_format | dspace |
| spelling | utm.eprints-449702017-09-19T07:55:05Z http://eprints.utm.my/44970/ Identification of genes involved in the 4-aminobenzenesulfote degradation pathway of hyrogenophaga sp.PBC via transposon mutagenesis Han, Ming Gan Ibrahim, Zaharah Shahir, Shafinaz Yahya, Adibah QH Natural history Genes involved in the 4-aminobenzenesulfonate (4-ABS) degradation pathway of Hydrogenophaga sp. PBC were identified using transposon mutagenesis. The screening of 10 000 mutants for incomplete 4-ABS biotransformation identified four mutants with single transposon insertion. Genes with insertions that impaired the ability to utilize 4-ABS for growth included (1) 4-sulfocatechol 1,2-dioxygenase b-subunit (pcaH2) and 3-sulfomuconate cycloisomerase involved in the modified b-ketoadipate pathway; (2) 4-aminobenzenesulfonate 3,4-dioxy- genase component (sadA) involved in aromatic ring hydroxylation; and (3) transposase gene homolog with a putative cis-diol dehydrogenase gene located downstream. The pcaH2 mutant strain accumulated brown metabolite during growth on 4-ABS which was identified as 4-sulfocatechol through thin layer chromatography and HPLC analyses. Supplementation of w ild-type sadA gene in trans restored the 4-ABS degradation ability of the sadA mutant, thus supporting the annotation of its disrupted gene. Oxford University Press 2011-05 Article PeerReviewed Han, Ming Gan and Ibrahim, Zaharah and Shahir, Shafinaz and Yahya, Adibah (2011) Identification of genes involved in the 4-aminobenzenesulfote degradation pathway of hyrogenophaga sp.PBC via transposon mutagenesis. FEMS Microbiology Letters, 318 (2). pp. 108-114. ISSN 0378-1097 http://dx.doi.org/10.1111/j.1574-6968.2011.02245.x DOI:10.1111/j.1574-6968.2011.02245.x |
| spellingShingle | QH Natural history Han, Ming Gan Ibrahim, Zaharah Shahir, Shafinaz Yahya, Adibah Identification of genes involved in the 4-aminobenzenesulfote degradation pathway of hyrogenophaga sp.PBC via transposon mutagenesis |
| title | Identification of genes involved in the 4-aminobenzenesulfote degradation pathway of hyrogenophaga sp.PBC via transposon mutagenesis |
| title_full | Identification of genes involved in the 4-aminobenzenesulfote degradation pathway of hyrogenophaga sp.PBC via transposon mutagenesis |
| title_fullStr | Identification of genes involved in the 4-aminobenzenesulfote degradation pathway of hyrogenophaga sp.PBC via transposon mutagenesis |
| title_full_unstemmed | Identification of genes involved in the 4-aminobenzenesulfote degradation pathway of hyrogenophaga sp.PBC via transposon mutagenesis |
| title_short | Identification of genes involved in the 4-aminobenzenesulfote degradation pathway of hyrogenophaga sp.PBC via transposon mutagenesis |
| title_sort | identification of genes involved in the 4 aminobenzenesulfote degradation pathway of hyrogenophaga sp pbc via transposon mutagenesis |
| topic | QH Natural history |
| work_keys_str_mv | AT hanminggan identificationofgenesinvolvedinthe4aminobenzenesulfotedegradationpathwayofhyrogenophagasppbcviatransposonmutagenesis AT ibrahimzaharah identificationofgenesinvolvedinthe4aminobenzenesulfotedegradationpathwayofhyrogenophagasppbcviatransposonmutagenesis AT shahirshafinaz identificationofgenesinvolvedinthe4aminobenzenesulfotedegradationpathwayofhyrogenophagasppbcviatransposonmutagenesis AT yahyaadibah identificationofgenesinvolvedinthe4aminobenzenesulfotedegradationpathwayofhyrogenophagasppbcviatransposonmutagenesis |