Review : overexpression of protein under transcriptional regulation of lambda pL promoter system in Escherichia coli: consequences and bioprocess improvement approaches
The attraction of employing thermal induction system in the production of heterologous protein is mainly due to 1) a higher expressed protein titer in comparison to constitutive system, and 2) a cleaner process since no chemical inducing agent is added. However, problems pertaining to high temperatu...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English English |
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Faculty of Chemical and Natural Resources Engineering, UTM
2007
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| Online Access: | http://eprints.utm.my/4633/1/FirdausiRazali2007_ReviewOverexpressionofProteinUnderTranscriptional.pdf http://eprints.utm.my/4633/2/vol%201%2022-39.pdf |
| _version_ | 1825909593308397568 |
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| author | Razali, Firdausi Moo-Young, Murray Scharer, Jeno M. Glick, Bernard R. |
| author_facet | Razali, Firdausi Moo-Young, Murray Scharer, Jeno M. Glick, Bernard R. |
| author_sort | Razali, Firdausi |
| collection | ePrints |
| description | The attraction of employing thermal induction system in the production of heterologous protein is mainly due to 1) a higher expressed protein titer in comparison to constitutive system, and 2) a cleaner process since no chemical inducing agent is added. However, problems pertaining to high temperature and protein overexpression such as inclusion bodies formations, metabolic overload on the host, stress-related proteolysis on the expressed protein, and enhanced acetic acid secretion are commonly encountered. Several bioprocess approaches that include temperature upshift schemes, feeding strategies, timing of induction, two-stage culture mode, and stabilization of plasmid had been implemented to minimize some of these problems. The approaches are reviewed in this article.
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| first_indexed | 2024-03-05T18:04:29Z |
| format | Article |
| id | utm.eprints-4633 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English English |
| last_indexed | 2024-03-05T18:04:29Z |
| publishDate | 2007 |
| publisher | Faculty of Chemical and Natural Resources Engineering, UTM |
| record_format | dspace |
| spelling | utm.eprints-46332014-01-15T00:55:11Z http://eprints.utm.my/4633/ Review : overexpression of protein under transcriptional regulation of lambda pL promoter system in Escherichia coli: consequences and bioprocess improvement approaches Razali, Firdausi Moo-Young, Murray Scharer, Jeno M. Glick, Bernard R. TP Chemical technology The attraction of employing thermal induction system in the production of heterologous protein is mainly due to 1) a higher expressed protein titer in comparison to constitutive system, and 2) a cleaner process since no chemical inducing agent is added. However, problems pertaining to high temperature and protein overexpression such as inclusion bodies formations, metabolic overload on the host, stress-related proteolysis on the expressed protein, and enhanced acetic acid secretion are commonly encountered. Several bioprocess approaches that include temperature upshift schemes, feeding strategies, timing of induction, two-stage culture mode, and stabilization of plasmid had been implemented to minimize some of these problems. The approaches are reviewed in this article. Faculty of Chemical and Natural Resources Engineering, UTM 2007-03 Article PeerReviewed application/pdf en http://eprints.utm.my/4633/1/FirdausiRazali2007_ReviewOverexpressionofProteinUnderTranscriptional.pdf text/html en http://eprints.utm.my/4633/2/vol%201%2022-39.pdf Razali, Firdausi and Moo-Young, Murray and Scharer, Jeno M. and Glick, Bernard R. (2007) Review : overexpression of protein under transcriptional regulation of lambda pL promoter system in Escherichia coli: consequences and bioprocess improvement approaches. Journal of Chemical and Natural Resources Engineering, 1 . pp. 22-39. ISSN 1823-5255 |
| spellingShingle | TP Chemical technology Razali, Firdausi Moo-Young, Murray Scharer, Jeno M. Glick, Bernard R. Review : overexpression of protein under transcriptional regulation of lambda pL promoter system in Escherichia coli: consequences and bioprocess improvement approaches |
| title | Review : overexpression of protein under transcriptional regulation of lambda pL promoter system in Escherichia coli: consequences and bioprocess improvement approaches |
| title_full | Review : overexpression of protein under transcriptional regulation of lambda pL promoter system in Escherichia coli: consequences and bioprocess improvement approaches |
| title_fullStr | Review : overexpression of protein under transcriptional regulation of lambda pL promoter system in Escherichia coli: consequences and bioprocess improvement approaches |
| title_full_unstemmed | Review : overexpression of protein under transcriptional regulation of lambda pL promoter system in Escherichia coli: consequences and bioprocess improvement approaches |
| title_short | Review : overexpression of protein under transcriptional regulation of lambda pL promoter system in Escherichia coli: consequences and bioprocess improvement approaches |
| title_sort | review overexpression of protein under transcriptional regulation of lambda pl promoter system in escherichia coli consequences and bioprocess improvement approaches |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/4633/1/FirdausiRazali2007_ReviewOverexpressionofProteinUnderTranscriptional.pdf http://eprints.utm.my/4633/2/vol%201%2022-39.pdf |
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