Microbial transformation and sorption of anthracene in liquid culture
Armillaria sp. F022, a white-rot fungus isolated from decayed wood in tropical rain forest was used to biodegrade anthracene in cultured medium. The percentage of anthracene removal by Armillaria sp. F022 reached 13 % after 7 days and at the end of the experiment, anthracene removal level was at 87...
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2012
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author | Hadibarata, T. Zubir, M. M. Rubiyatno, Rubiyatno Chuang, T. Z. |
author_facet | Hadibarata, T. Zubir, M. M. Rubiyatno, Rubiyatno Chuang, T. Z. |
author_sort | Hadibarata, T. |
collection | ePrints |
description | Armillaria sp. F022, a white-rot fungus isolated from decayed wood in tropical rain forest was used to biodegrade anthracene in cultured medium. The percentage of anthracene removal by Armillaria sp. F022 reached 13 % after 7 days and at the end of the experiment, anthracene removal level was at 87 %. The anthracene removal through sorption and transformation was investigated. 69 % of eliminated anthracene was transformed by Armillaria sp. F022 to form other organic structure, while only 18 % was absorbed in the mycelia. In the kinetic experiment, anthracene dissipation will not stop even though the biomass had stopped growing. Anthracene removal by Armillaria sp. F022 was correlated with protein concentration (whole biomass) in the culture. The production of enzyme was affected by biomass production. Anthracene was transformed to two stable metabolic products. The metabolites were extracted in ethyl-acetate, isolated by column chromatography, and then identified using gas chromatography-mass spectrometry (GC-MS). |
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format | Article |
id | utm.eprints-47209 |
institution | Universiti Teknologi Malaysia - ePrints |
last_indexed | 2024-03-05T19:22:16Z |
publishDate | 2012 |
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spelling | utm.eprints-472092019-03-31T08:34:49Z http://eprints.utm.my/47209/ Microbial transformation and sorption of anthracene in liquid culture Hadibarata, T. Zubir, M. M. Rubiyatno, Rubiyatno Chuang, T. Z. TP Chemical technology Armillaria sp. F022, a white-rot fungus isolated from decayed wood in tropical rain forest was used to biodegrade anthracene in cultured medium. The percentage of anthracene removal by Armillaria sp. F022 reached 13 % after 7 days and at the end of the experiment, anthracene removal level was at 87 %. The anthracene removal through sorption and transformation was investigated. 69 % of eliminated anthracene was transformed by Armillaria sp. F022 to form other organic structure, while only 18 % was absorbed in the mycelia. In the kinetic experiment, anthracene dissipation will not stop even though the biomass had stopped growing. Anthracene removal by Armillaria sp. F022 was correlated with protein concentration (whole biomass) in the culture. The production of enzyme was affected by biomass production. Anthracene was transformed to two stable metabolic products. The metabolites were extracted in ethyl-acetate, isolated by column chromatography, and then identified using gas chromatography-mass spectrometry (GC-MS). 2012 Article PeerReviewed Hadibarata, T. and Zubir, M. M. and Rubiyatno, Rubiyatno and Chuang, T. Z. (2012) Microbial transformation and sorption of anthracene in liquid culture. Bioprocess and Biosystems Engineering, 36 (9). pp. 1-5. ISSN 1615-7591 http://dx.doi.org/10.1007/s00449-012-0850-x DOI:10.1007/s00449-012-0850-x |
spellingShingle | TP Chemical technology Hadibarata, T. Zubir, M. M. Rubiyatno, Rubiyatno Chuang, T. Z. Microbial transformation and sorption of anthracene in liquid culture |
title | Microbial transformation and sorption of anthracene in liquid culture |
title_full | Microbial transformation and sorption of anthracene in liquid culture |
title_fullStr | Microbial transformation and sorption of anthracene in liquid culture |
title_full_unstemmed | Microbial transformation and sorption of anthracene in liquid culture |
title_short | Microbial transformation and sorption of anthracene in liquid culture |
title_sort | microbial transformation and sorption of anthracene in liquid culture |
topic | TP Chemical technology |
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