Preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation
This study was aimed to investigate the production of recombinant cellobiohydrolase (CBH) in static liquid culture (SLC) fermentation using newly developed recombinant of A. niger. Different environmental parameters and culture conditions were used to attain good cultivation of A. niger in SLC ferme...
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| Format: | Article |
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2012
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| author | Md. Noh, Abidah Mohamad, Siti Hajar Md. Jahim, Jamaliah Abdul Murad, Abdul Munir Abu Bakar, Farah Diba Muhammad, Nor Mahadi, Mahadi Md. Illias, Rosli |
| author_facet | Md. Noh, Abidah Mohamad, Siti Hajar Md. Jahim, Jamaliah Abdul Murad, Abdul Munir Abu Bakar, Farah Diba Muhammad, Nor Mahadi, Mahadi Md. Illias, Rosli |
| author_sort | Md. Noh, Abidah |
| collection | ePrints |
| description | This study was aimed to investigate the production of recombinant cellobiohydrolase (CBH) in static liquid culture (SLC) fermentation using newly developed recombinant of A. niger. Different environmental parameters and culture conditions were used to attain good cultivation of A. niger in SLC fermentation and highest level of CBH production. The fermentation results were evaluated from CBH production, total biomass, total protein and the residual main carbon source, maltose after 9 days of cultivation. The CBH production was found effectively enhanced under optimized cultivation temperature, initial pH, inoculums size and pre-shaked inoculums. Maximum biomass, protein concentration and enzyme activity were determined as 1.5 g dry weight/ml medium, 3.5 mg/ml and 40 U/ml respectively. The results also show that SLC could enhance production of the recombinant CBH. |
| first_indexed | 2024-03-05T19:22:45Z |
| format | Article |
| id | utm.eprints-47403 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:22:45Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | utm.eprints-474032019-04-25T01:18:40Z http://eprints.utm.my/47403/ Preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation Md. Noh, Abidah Mohamad, Siti Hajar Md. Jahim, Jamaliah Abdul Murad, Abdul Munir Abu Bakar, Farah Diba Muhammad, Nor Mahadi, Mahadi Md. Illias, Rosli Q Science This study was aimed to investigate the production of recombinant cellobiohydrolase (CBH) in static liquid culture (SLC) fermentation using newly developed recombinant of A. niger. Different environmental parameters and culture conditions were used to attain good cultivation of A. niger in SLC fermentation and highest level of CBH production. The fermentation results were evaluated from CBH production, total biomass, total protein and the residual main carbon source, maltose after 9 days of cultivation. The CBH production was found effectively enhanced under optimized cultivation temperature, initial pH, inoculums size and pre-shaked inoculums. Maximum biomass, protein concentration and enzyme activity were determined as 1.5 g dry weight/ml medium, 3.5 mg/ml and 40 U/ml respectively. The results also show that SLC could enhance production of the recombinant CBH. 2012-01-01 Article PeerReviewed Md. Noh, Abidah and Mohamad, Siti Hajar and Md. Jahim, Jamaliah and Abdul Murad, Abdul Munir and Abu Bakar, Farah Diba and Muhammad, Nor and Mahadi, Mahadi and Md. Illias, Rosli (2012) Preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation. Australian Journal Of Basic And Applied Sciences, 6 (1). pp. 43-49. ISSN 1991-8178 http://www.ajbasweb.com/old/ajbas/2012/January/43-49.pdf |
| spellingShingle | Q Science Md. Noh, Abidah Mohamad, Siti Hajar Md. Jahim, Jamaliah Abdul Murad, Abdul Munir Abu Bakar, Farah Diba Muhammad, Nor Mahadi, Mahadi Md. Illias, Rosli Preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation |
| title | Preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation |
| title_full | Preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation |
| title_fullStr | Preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation |
| title_full_unstemmed | Preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation |
| title_short | Preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation |
| title_sort | preliminary study of cellobiohydrolase production from aspergillus niger using static liquid culture fermentation |
| topic | Q Science |
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