Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis
Fifty signal peptides of Pediococcus pentosaceus were characterized by in silico analysis and, based on the physicochemical analysis, (two potential signal peptides Spk1 and Spk3 were identified). The coding sequences of SP were amplified and fused to the gene coding for green fluorescent protein (G...
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| Format: | Article |
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Springer Science+Business Media Dordrecht
2013
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| _version_ | 1796859430494535680 |
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| author | Baradaran, Ali Sieo, Chin Chin Foo, Hooi Ling Md. Illias, Rosli Yusoff, Khatijah Abdul Rahim, Raha |
| author_facet | Baradaran, Ali Sieo, Chin Chin Foo, Hooi Ling Md. Illias, Rosli Yusoff, Khatijah Abdul Rahim, Raha |
| author_sort | Baradaran, Ali |
| collection | ePrints |
| description | Fifty signal peptides of Pediococcus pentosaceus were characterized by in silico analysis and, based on the physicochemical analysis, (two potential signal peptides Spk1 and Spk3 were identified). The coding sequences of SP were amplified and fused to the gene coding for green fluorescent protein (GFP) and cloned into Lactococcus lactis pNZ8048 and pMG36e vectors, respectively. Western blot analysis indicated that the GFP proteins were secreted using both heterologous SPs. ELISA showed that the secretion efficiency of GFP using Spk1 (0.64 μg/ml) was similar to using Usp45 (0.62 μg/ml) and Spk3 (0.58 μg/ml). |
| first_indexed | 2024-03-05T19:27:03Z |
| format | Article |
| id | utm.eprints-49249 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:27:03Z |
| publishDate | 2013 |
| publisher | Springer Science+Business Media Dordrecht |
| record_format | dspace |
| spelling | utm.eprints-492492019-01-28T04:30:25Z http://eprints.utm.my/49249/ Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis Baradaran, Ali Sieo, Chin Chin Foo, Hooi Ling Md. Illias, Rosli Yusoff, Khatijah Abdul Rahim, Raha TP Chemical technology Fifty signal peptides of Pediococcus pentosaceus were characterized by in silico analysis and, based on the physicochemical analysis, (two potential signal peptides Spk1 and Spk3 were identified). The coding sequences of SP were amplified and fused to the gene coding for green fluorescent protein (GFP) and cloned into Lactococcus lactis pNZ8048 and pMG36e vectors, respectively. Western blot analysis indicated that the GFP proteins were secreted using both heterologous SPs. ELISA showed that the secretion efficiency of GFP using Spk1 (0.64 μg/ml) was similar to using Usp45 (0.62 μg/ml) and Spk3 (0.58 μg/ml). Springer Science+Business Media Dordrecht 2013 Article PeerReviewed Baradaran, Ali and Sieo, Chin Chin and Foo, Hooi Ling and Md. Illias, Rosli and Yusoff, Khatijah and Abdul Rahim, Raha (2013) Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis. Biotechnology Letters, 35 (2). pp. 233-238. ISSN 0141-5492 https://doi.org/10.1007/s10529-012-1059-4 DOI: 10.1007/s10529-012-1059-4 |
| spellingShingle | TP Chemical technology Baradaran, Ali Sieo, Chin Chin Foo, Hooi Ling Md. Illias, Rosli Yusoff, Khatijah Abdul Rahim, Raha Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis |
| title | Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis |
| title_full | Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis |
| title_fullStr | Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis |
| title_full_unstemmed | Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis |
| title_short | Cloning and in silico characterization of two signal peptides from Pediococcus pentosaceus and their function for the secretion of heterologous protein in Lactococcus lactis |
| title_sort | cloning and in silico characterization of two signal peptides from pediococcus pentosaceus and their function for the secretion of heterologous protein in lactococcus lactis |
| topic | TP Chemical technology |
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