A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1
Bacillus lehensis G1 is a Gram-positive, moderately alkalitolerant bacterium isolated from soil samples. B. lehensis produces cyclodextrin glucanotransferase (CGTase), an enzyme that has enabled the extensive use of cyclodextrin in foodstuffs, chemicals, and pharmaceuticals. The genome sequence of B...
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Elsevier B.V.
2014
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| author | Noor, Y. M. Samsulrizal, Nurul Hidayah Jema'on, Noor Azah Low, Kheng Oon Ramli, Aizi Nor Mazila Alias, Noor Izawati Darnis, Siti Intan Rosdianah Mohamad Fuzi, Siti Fatimah Zaharah Mat Isa, Mohd. Noor Abd. Murad, Abd. Munir Mohd. Raih, Mohd. Firdaus Abu Bakar, Farah Diba Najimudin, Nazalan Mahadi, Nor Muhammad Md. Illias, Rosli |
| author_facet | Noor, Y. M. Samsulrizal, Nurul Hidayah Jema'on, Noor Azah Low, Kheng Oon Ramli, Aizi Nor Mazila Alias, Noor Izawati Darnis, Siti Intan Rosdianah Mohamad Fuzi, Siti Fatimah Zaharah Mat Isa, Mohd. Noor Abd. Murad, Abd. Munir Mohd. Raih, Mohd. Firdaus Abu Bakar, Farah Diba Najimudin, Nazalan Mahadi, Nor Muhammad Md. Illias, Rosli |
| author_sort | Noor, Y. M. |
| collection | ePrints |
| description | Bacillus lehensis G1 is a Gram-positive, moderately alkalitolerant bacterium isolated from soil samples. B. lehensis produces cyclodextrin glucanotransferase (CGTase), an enzyme that has enabled the extensive use of cyclodextrin in foodstuffs, chemicals, and pharmaceuticals. The genome sequence of B. lehensis G1 consists of a single circular 3.99 Mb chromosome containing 4017 protein-coding sequences (CDSs), of which 2818 (70.15%) have assigned biological roles, 936 (23.30%) have conserved domains with unknown functions, and 263 (6.55%) have no match with any protein database. Bacillus clausii KSM-K16 was established as the closest relative to B. lehensis G1 based on gene content similarity and 16S rRNA phylogenetic analysis. A total of 2820 proteins from B. lehensis G1 were found to have orthologues in B. clausii, including sodium-proton antiporters, transport proteins, and proteins involved in ATP synthesis. A comparative analysis of these proteins and those in B. clausii and other alkaliphilic Bacillus species was carried out to investigate their contributions towards the alkalitolerance of the microorganism. The similarities and differences in alkalitolerance-related genes among alkalitolerant/alkaliphilic Bacillus species highlight the complex mechanism of pH homeostasis. The B. lehensis G1 genome was also mined for proteins and enzymes with potential viability for industrial and commercial purposes. |
| first_indexed | 2024-03-05T19:30:13Z |
| format | Article |
| id | utm.eprints-51450 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:30:13Z |
| publishDate | 2014 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | utm.eprints-514502019-01-28T04:30:41Z http://eprints.utm.my/51450/ A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1 Noor, Y. M. Samsulrizal, Nurul Hidayah Jema'on, Noor Azah Low, Kheng Oon Ramli, Aizi Nor Mazila Alias, Noor Izawati Darnis, Siti Intan Rosdianah Mohamad Fuzi, Siti Fatimah Zaharah Mat Isa, Mohd. Noor Abd. Murad, Abd. Munir Mohd. Raih, Mohd. Firdaus Abu Bakar, Farah Diba Najimudin, Nazalan Mahadi, Nor Muhammad Md. Illias, Rosli TP Chemical technology Bacillus lehensis G1 is a Gram-positive, moderately alkalitolerant bacterium isolated from soil samples. B. lehensis produces cyclodextrin glucanotransferase (CGTase), an enzyme that has enabled the extensive use of cyclodextrin in foodstuffs, chemicals, and pharmaceuticals. The genome sequence of B. lehensis G1 consists of a single circular 3.99 Mb chromosome containing 4017 protein-coding sequences (CDSs), of which 2818 (70.15%) have assigned biological roles, 936 (23.30%) have conserved domains with unknown functions, and 263 (6.55%) have no match with any protein database. Bacillus clausii KSM-K16 was established as the closest relative to B. lehensis G1 based on gene content similarity and 16S rRNA phylogenetic analysis. A total of 2820 proteins from B. lehensis G1 were found to have orthologues in B. clausii, including sodium-proton antiporters, transport proteins, and proteins involved in ATP synthesis. A comparative analysis of these proteins and those in B. clausii and other alkaliphilic Bacillus species was carried out to investigate their contributions towards the alkalitolerance of the microorganism. The similarities and differences in alkalitolerance-related genes among alkalitolerant/alkaliphilic Bacillus species highlight the complex mechanism of pH homeostasis. The B. lehensis G1 genome was also mined for proteins and enzymes with potential viability for industrial and commercial purposes. Elsevier B.V. 2014-05-15 Article PeerReviewed Noor, Y. M. and Samsulrizal, Nurul Hidayah and Jema'on, Noor Azah and Low, Kheng Oon and Ramli, Aizi Nor Mazila and Alias, Noor Izawati and Darnis, Siti Intan Rosdianah and Mohamad Fuzi, Siti Fatimah Zaharah and Mat Isa, Mohd. Noor and Abd. Murad, Abd. Munir and Mohd. Raih, Mohd. Firdaus and Abu Bakar, Farah Diba and Najimudin, Nazalan and Mahadi, Nor Muhammad and Md. Illias, Rosli (2014) A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1. Gene, 545 (2). pp. 253-261. ISSN 1879-0038 http://dx.doi.org/10.1016/j.gene.2014.05.012 DOI: 10.1016/j.gene.2014.05.012 |
| spellingShingle | TP Chemical technology Noor, Y. M. Samsulrizal, Nurul Hidayah Jema'on, Noor Azah Low, Kheng Oon Ramli, Aizi Nor Mazila Alias, Noor Izawati Darnis, Siti Intan Rosdianah Mohamad Fuzi, Siti Fatimah Zaharah Mat Isa, Mohd. Noor Abd. Murad, Abd. Munir Mohd. Raih, Mohd. Firdaus Abu Bakar, Farah Diba Najimudin, Nazalan Mahadi, Nor Muhammad Md. Illias, Rosli A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1 |
| title | A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1 |
| title_full | A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1 |
| title_fullStr | A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1 |
| title_full_unstemmed | A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1 |
| title_short | A comparative genomic analysis of the alkalitolerant soil bacterium Bacillus lehensis G1 |
| title_sort | comparative genomic analysis of the alkalitolerant soil bacterium bacillus lehensis g1 |
| topic | TP Chemical technology |
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