Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3)
Phytase production using Escherichia coli BL21 (DE3), a recombinant strainharboring a plasmid encoding thermostable Bacillus phytase, in semi-industrial scalewas studied in this work. Among the factors needed to be considered in order to achievehigh enzyme yield, aeration rate plays an important rol...
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| Format: | Article |
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Journal of Pure and Applied Microbiology
2014
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| _version_ | 1796859655429816320 |
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| author | Nor Zalina, Othman Elsayed A., Elsayed Abd. Malek, Roslinda Solleh, Ramli Masri, Helmy J. Sarmidi, Mohamad Roji Abdul Aziz, Ramlan Wadaan, Mohamad A. Hatti-Kaul, Rajni Hesham A., El Enshasy |
| author_facet | Nor Zalina, Othman Elsayed A., Elsayed Abd. Malek, Roslinda Solleh, Ramli Masri, Helmy J. Sarmidi, Mohamad Roji Abdul Aziz, Ramlan Wadaan, Mohamad A. Hatti-Kaul, Rajni Hesham A., El Enshasy |
| author_sort | Nor Zalina, Othman |
| collection | ePrints |
| description | Phytase production using Escherichia coli BL21 (DE3), a recombinant strainharboring a plasmid encoding thermostable Bacillus phytase, in semi-industrial scalewas studied in this work. Among the factors needed to be considered in order to achievehigh enzyme yield, aeration rate plays an important role. Suitable aeration is required tosupply cells with sufficient amount of air for cell and phytase production. The effect of different aeration rates, (1.0, 2.0 and 3.0 vv -1 min -1 ), on the kinetics of cell growth andphytase production by the recombinant E. coli BL21 (DE3) in 16-L pilot scale stirred tankbioreactor was investigated. The highest cell concentration of 3.81 gL -1 concomitant withthe maximal total phytase production of 15.63 UmL -1 were obtained in a bioreactor of aeration rate 3.0vv -1 min -1 . At this high aeration rate, a maximal specific growth rate (µ max )and the maximal specific phytase production of 0.33 h -1 and 4102Ug -1 , respectively, wereachieved |
| first_indexed | 2024-03-05T19:30:23Z |
| format | Article |
| id | utm.eprints-51737 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:30:23Z |
| publishDate | 2014 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | dspace |
| spelling | utm.eprints-517372018-10-16T07:30:31Z http://eprints.utm.my/51737/ Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3) Nor Zalina, Othman Elsayed A., Elsayed Abd. Malek, Roslinda Solleh, Ramli Masri, Helmy J. Sarmidi, Mohamad Roji Abdul Aziz, Ramlan Wadaan, Mohamad A. Hatti-Kaul, Rajni Hesham A., El Enshasy TP Chemical technology Phytase production using Escherichia coli BL21 (DE3), a recombinant strainharboring a plasmid encoding thermostable Bacillus phytase, in semi-industrial scalewas studied in this work. Among the factors needed to be considered in order to achievehigh enzyme yield, aeration rate plays an important role. Suitable aeration is required tosupply cells with sufficient amount of air for cell and phytase production. The effect of different aeration rates, (1.0, 2.0 and 3.0 vv -1 min -1 ), on the kinetics of cell growth andphytase production by the recombinant E. coli BL21 (DE3) in 16-L pilot scale stirred tankbioreactor was investigated. The highest cell concentration of 3.81 gL -1 concomitant withthe maximal total phytase production of 15.63 UmL -1 were obtained in a bioreactor of aeration rate 3.0vv -1 min -1 . At this high aeration rate, a maximal specific growth rate (µ max )and the maximal specific phytase production of 0.33 h -1 and 4102Ug -1 , respectively, wereachieved Journal of Pure and Applied Microbiology 2014 Article PeerReviewed Nor Zalina, Othman and Elsayed A., Elsayed and Abd. Malek, Roslinda and Solleh, Ramli and Masri, Helmy J. and Sarmidi, Mohamad Roji and Abdul Aziz, Ramlan and Wadaan, Mohamad A. and Hatti-Kaul, Rajni and Hesham A., El Enshasy (2014) Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3). Journal of Pure and Applied Microbiology, 8 (4). pp. 2721-2728. ISSN 0973-7510 http://www.academia.edu/8232923/Aeration_Rate_Effect_on_the_Growth_Kinetics_Phytase_Production_and_Plasmid_Stability_of_Recombinant_Escherichia_coli_BL21_DE3_ |
| spellingShingle | TP Chemical technology Nor Zalina, Othman Elsayed A., Elsayed Abd. Malek, Roslinda Solleh, Ramli Masri, Helmy J. Sarmidi, Mohamad Roji Abdul Aziz, Ramlan Wadaan, Mohamad A. Hatti-Kaul, Rajni Hesham A., El Enshasy Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3) |
| title | Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3) |
| title_full | Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3) |
| title_fullStr | Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3) |
| title_full_unstemmed | Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3) |
| title_short | Aeration rate effect on the growth kinetics, phytase production and plasmid stability of recombinant Escherichia coli BL21 (DE3) |
| title_sort | aeration rate effect on the growth kinetics phytase production and plasmid stability of recombinant escherichia coli bl21 de3 |
| topic | TP Chemical technology |
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