Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis
Lactococcus lactis is the most studied mesophilic fermentative lactic acid bacterium. It is used extensively in the food industry and plays a pivotal role as a cell factory and also as vaccine delivery platforms. The proteome of the Malaysian isolated L. lactis M4 dairy strain, obtained from the mil...
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| Format: | Article |
| Language: | English |
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ScientificWorld Ltd.
2014
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| Online Access: | http://eprints.utm.my/53007/1/AmirRabu2014_GrowthPhase-DependentProteomesoftheMalaysianIsolated.pdf |
| _version_ | 1796859809147912192 |
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| author | Yap, Theresa Wan Chen Rabu, Amir Abu Bakar, Farah Diba Abdul Rahim, Raha Md. Illias, Rosli Mahadi, Nor Muhammad Abdul Murad, Abdul Munir |
| author_facet | Yap, Theresa Wan Chen Rabu, Amir Abu Bakar, Farah Diba Abdul Rahim, Raha Md. Illias, Rosli Mahadi, Nor Muhammad Abdul Murad, Abdul Munir |
| author_sort | Yap, Theresa Wan Chen |
| collection | ePrints |
| description | Lactococcus lactis is the most studied mesophilic fermentative lactic acid bacterium. It is used extensively in the food industry and plays a pivotal role as a cell factory and also as vaccine delivery platforms. The proteome of the Malaysian isolated L. lactis M4 dairy strain, obtained from the milk of locally bred cows, was studied to elucidate the physiological changes occurring between the growth phases of this bacterium. In this study, ultraperformance liquid chromatography nanoflow electrospray ionization tandem mass spectrometry (UPLC- nano-ESI-MSE) approach was used for qualitative proteomic analysis. A total of 100 and 121 proteins were identified from the midexponential and early stationary growth phases, respectively, of the L. lactis strain M4. During the exponential phase, the most important reaction was the generation of sufficient energy, whereas, in the early stationary phase, the metabolic energy pathways decreased and the biosynthesis of proteins became more important. Thus, the metabolism of the cells shifted from energy production in the exponential phase to the synthesis of macromolecules in the stationary phase. The resultant proteomes are essential in providing an improved view of the cellular machinery of L. lactis during the transition of growth phases and hence provide insight into various biotechnological applications. |
| first_indexed | 2024-03-05T19:32:43Z |
| format | Article |
| id | utm.eprints-53007 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T19:32:43Z |
| publishDate | 2014 |
| publisher | ScientificWorld Ltd. |
| record_format | dspace |
| spelling | utm.eprints-530072018-07-19T07:23:26Z http://eprints.utm.my/53007/ Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis Yap, Theresa Wan Chen Rabu, Amir Abu Bakar, Farah Diba Abdul Rahim, Raha Md. Illias, Rosli Mahadi, Nor Muhammad Abdul Murad, Abdul Munir TP Chemical technology Lactococcus lactis is the most studied mesophilic fermentative lactic acid bacterium. It is used extensively in the food industry and plays a pivotal role as a cell factory and also as vaccine delivery platforms. The proteome of the Malaysian isolated L. lactis M4 dairy strain, obtained from the milk of locally bred cows, was studied to elucidate the physiological changes occurring between the growth phases of this bacterium. In this study, ultraperformance liquid chromatography nanoflow electrospray ionization tandem mass spectrometry (UPLC- nano-ESI-MSE) approach was used for qualitative proteomic analysis. A total of 100 and 121 proteins were identified from the midexponential and early stationary growth phases, respectively, of the L. lactis strain M4. During the exponential phase, the most important reaction was the generation of sufficient energy, whereas, in the early stationary phase, the metabolic energy pathways decreased and the biosynthesis of proteins became more important. Thus, the metabolism of the cells shifted from energy production in the exponential phase to the synthesis of macromolecules in the stationary phase. The resultant proteomes are essential in providing an improved view of the cellular machinery of L. lactis during the transition of growth phases and hence provide insight into various biotechnological applications. ScientificWorld Ltd. 2014 Article PeerReviewed application/pdf en http://eprints.utm.my/53007/1/AmirRabu2014_GrowthPhase-DependentProteomesoftheMalaysianIsolated.pdf Yap, Theresa Wan Chen and Rabu, Amir and Abu Bakar, Farah Diba and Abdul Rahim, Raha and Md. Illias, Rosli and Mahadi, Nor Muhammad and Abdul Murad, Abdul Munir (2014) Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis. Scientific World Journal . ISSN 1537-744X http://dx.doi.org/10.1155/2014/642891 DOI: 10.1155/2014/642891 |
| spellingShingle | TP Chemical technology Yap, Theresa Wan Chen Rabu, Amir Abu Bakar, Farah Diba Abdul Rahim, Raha Md. Illias, Rosli Mahadi, Nor Muhammad Abdul Murad, Abdul Munir Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis |
| title | Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis |
| title_full | Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis |
| title_fullStr | Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis |
| title_full_unstemmed | Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis |
| title_short | Growth phase-dependent proteomes of the malaysian isolated lactococcus lactis dairy strain M4 using label-free qualitative shotgun proteomics analysis |
| title_sort | growth phase dependent proteomes of the malaysian isolated lactococcus lactis dairy strain m4 using label free qualitative shotgun proteomics analysis |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/53007/1/AmirRabu2014_GrowthPhase-DependentProteomesoftheMalaysianIsolated.pdf |
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