Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes
Diclofenac is recently considered as one of the most devastating environmental pollutants, because of its biomagnification in the food chain which leads to potential harmful effects on non-targeted organisms. This study describes the optimized laccase mediated diclofenac transformation using respons...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The Royal Society of Chemistry
2014
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| Online Access: | http://eprints.utm.my/53295/1/PalanivelSathishkumar2014_Laccasemediateddiclofenactransformation.pdf |
| _version_ | 1796859824487530496 |
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| author | Sathishkumar, Palanivel Mythili, Arumugam Hadibarata, Tony Jayakumar, Rajarajeswaran Kanthimathi, M. S. Thayumanavan, Palvannan Ponraj, Mohanadoss Salim, Mohd. Razman Mohd. Yusoff, Abdull Rahim |
| author_facet | Sathishkumar, Palanivel Mythili, Arumugam Hadibarata, Tony Jayakumar, Rajarajeswaran Kanthimathi, M. S. Thayumanavan, Palvannan Ponraj, Mohanadoss Salim, Mohd. Razman Mohd. Yusoff, Abdull Rahim |
| author_sort | Sathishkumar, Palanivel |
| collection | ePrints |
| description | Diclofenac is recently considered as one of the most devastating environmental pollutants, because of its biomagnification in the food chain which leads to potential harmful effects on non-targeted organisms. This study describes the optimized laccase mediated diclofenac transformation using response surface methodology (RSM) and cytotoxicity testing on mouse fibroblast 3T3-L1 preadipocytes. Three factors (laccase, syringaldehyde, reaction time) were used to optimize the diclofenac transformation. The optimum level of laccase, syringaldehyde and reaction time was found to be 1.91 U mL -1, 187 μg and 51 min for diclofenac transformation (20 mg L -1). The cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes showed that a maximum of 67.9% cell death occurred at 72 h treatment with diclofenac (200 μg mL-1), while the cells treated with laccase treated diclofenac (LTD) showed less toxicity on the cells. These findings can be addressed for the removal of diclofenac toxicity |
| first_indexed | 2024-03-05T19:32:57Z |
| format | Article |
| id | utm.eprints-53295 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T19:32:57Z |
| publishDate | 2014 |
| publisher | The Royal Society of Chemistry |
| record_format | dspace |
| spelling | utm.eprints-532952018-07-25T07:55:25Z http://eprints.utm.my/53295/ Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes Sathishkumar, Palanivel Mythili, Arumugam Hadibarata, Tony Jayakumar, Rajarajeswaran Kanthimathi, M. S. Thayumanavan, Palvannan Ponraj, Mohanadoss Salim, Mohd. Razman Mohd. Yusoff, Abdull Rahim TA Engineering (General). Civil engineering (General) Diclofenac is recently considered as one of the most devastating environmental pollutants, because of its biomagnification in the food chain which leads to potential harmful effects on non-targeted organisms. This study describes the optimized laccase mediated diclofenac transformation using response surface methodology (RSM) and cytotoxicity testing on mouse fibroblast 3T3-L1 preadipocytes. Three factors (laccase, syringaldehyde, reaction time) were used to optimize the diclofenac transformation. The optimum level of laccase, syringaldehyde and reaction time was found to be 1.91 U mL -1, 187 μg and 51 min for diclofenac transformation (20 mg L -1). The cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes showed that a maximum of 67.9% cell death occurred at 72 h treatment with diclofenac (200 μg mL-1), while the cells treated with laccase treated diclofenac (LTD) showed less toxicity on the cells. These findings can be addressed for the removal of diclofenac toxicity The Royal Society of Chemistry 2014 Article PeerReviewed application/pdf en http://eprints.utm.my/53295/1/PalanivelSathishkumar2014_Laccasemediateddiclofenactransformation.pdf Sathishkumar, Palanivel and Mythili, Arumugam and Hadibarata, Tony and Jayakumar, Rajarajeswaran and Kanthimathi, M. S. and Thayumanavan, Palvannan and Ponraj, Mohanadoss and Salim, Mohd. Razman and Mohd. Yusoff, Abdull Rahim (2014) Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes. RSC Advances, 4 (23). pp. 11689-11697. ISSN 2046-2069 http://dx.doi.org/10.1039/c3ra46014b DOI: 10.1039/c3ra46014b |
| spellingShingle | TA Engineering (General). Civil engineering (General) Sathishkumar, Palanivel Mythili, Arumugam Hadibarata, Tony Jayakumar, Rajarajeswaran Kanthimathi, M. S. Thayumanavan, Palvannan Ponraj, Mohanadoss Salim, Mohd. Razman Mohd. Yusoff, Abdull Rahim Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes |
| title | Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes |
| title_full | Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes |
| title_fullStr | Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes |
| title_full_unstemmed | Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes |
| title_short | Laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3T3-L1 preadipocytes |
| title_sort | laccase mediated diclofenac transformation and cytotoxicity assessment on mouse fibroblast 3t3 l1 preadipocytes |
| topic | TA Engineering (General). Civil engineering (General) |
| url | http://eprints.utm.my/53295/1/PalanivelSathishkumar2014_Laccasemediateddiclofenactransformation.pdf |
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