Preliminary study of antimicrobial (AM) effects of starch-based film incorporated with nisin, lysozymes and lauric acid

Active Packaging (AP) concept is a current trend in food packaging industries. An antimicrobial (AM) Active Packaging can be made by incorporating and immobilizing suitable AM agents into food packages and applying a bio switch concept. By that, the mechanism of antimicrobial release between the dev...

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Main Authors: Khairuddin, Nozieana, Muhamad, Ida Idayu
Format: Conference or Workshop Item
Language:English
Published: 2005
Subjects:
Online Access:http://eprints.utm.my/5358/1/NozieanaKhairuddin2005_PreliminaryStudyOfAntimicrobial%28AM%29.pdf
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author Khairuddin, Nozieana
Muhamad, Ida Idayu
author_facet Khairuddin, Nozieana
Muhamad, Ida Idayu
author_sort Khairuddin, Nozieana
collection ePrints
description Active Packaging (AP) concept is a current trend in food packaging industries. An antimicrobial (AM) Active Packaging can be made by incorporating and immobilizing suitable AM agents into food packages and applying a bio switch concept. By that, the mechanism of antimicrobial release between the developed bio-switch particles and the stimulus of a microbial contamination can be studied. A starch-based film was prepared and incorporated with antimicrobial agents, i.e. nisin, lysozyme and lauric acid. This film was then inoculated with the bacteria Escherichia Coli to carry out the microbial contamination study. The inhibition of E. Coli by the AM film was clearly observed in the broth and culture agar test. The decreased of optical density (OD 600nm) showed the inhibition of E. Coli growth. While, the clear zones formed on the film appearance showed that AM agents give good inhibition to the growth of E. Coli with satisfying inhibition rate. Lysozyme-incorporated film shows better inhibition than the other two in both tests.
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spelling utm.eprints-53582017-08-29T01:03:36Z http://eprints.utm.my/5358/ Preliminary study of antimicrobial (AM) effects of starch-based film incorporated with nisin, lysozymes and lauric acid Khairuddin, Nozieana Muhamad, Ida Idayu T Technology (General) Active Packaging (AP) concept is a current trend in food packaging industries. An antimicrobial (AM) Active Packaging can be made by incorporating and immobilizing suitable AM agents into food packages and applying a bio switch concept. By that, the mechanism of antimicrobial release between the developed bio-switch particles and the stimulus of a microbial contamination can be studied. A starch-based film was prepared and incorporated with antimicrobial agents, i.e. nisin, lysozyme and lauric acid. This film was then inoculated with the bacteria Escherichia Coli to carry out the microbial contamination study. The inhibition of E. Coli by the AM film was clearly observed in the broth and culture agar test. The decreased of optical density (OD 600nm) showed the inhibition of E. Coli growth. While, the clear zones formed on the film appearance showed that AM agents give good inhibition to the growth of E. Coli with satisfying inhibition rate. Lysozyme-incorporated film shows better inhibition than the other two in both tests. 2005 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/5358/1/NozieanaKhairuddin2005_PreliminaryStudyOfAntimicrobial%28AM%29.pdf Khairuddin, Nozieana and Muhamad, Ida Idayu (2005) Preliminary study of antimicrobial (AM) effects of starch-based film incorporated with nisin, lysozymes and lauric acid. In: Simposium Kimia Analisis Malaysia SKAM 18, 12-14 September 2005, Hotel Hyatt RegencyJohor Bahru, Johor.
spellingShingle T Technology (General)
Khairuddin, Nozieana
Muhamad, Ida Idayu
Preliminary study of antimicrobial (AM) effects of starch-based film incorporated with nisin, lysozymes and lauric acid
title Preliminary study of antimicrobial (AM) effects of starch-based film incorporated with nisin, lysozymes and lauric acid
title_full Preliminary study of antimicrobial (AM) effects of starch-based film incorporated with nisin, lysozymes and lauric acid
title_fullStr Preliminary study of antimicrobial (AM) effects of starch-based film incorporated with nisin, lysozymes and lauric acid
title_full_unstemmed Preliminary study of antimicrobial (AM) effects of starch-based film incorporated with nisin, lysozymes and lauric acid
title_short Preliminary study of antimicrobial (AM) effects of starch-based film incorporated with nisin, lysozymes and lauric acid
title_sort preliminary study of antimicrobial am effects of starch based film incorporated with nisin lysozymes and lauric acid
topic T Technology (General)
url http://eprints.utm.my/5358/1/NozieanaKhairuddin2005_PreliminaryStudyOfAntimicrobial%28AM%29.pdf
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AT muhamadidaidayu preliminarystudyofantimicrobialameffectsofstarchbasedfilmincorporatedwithnisinlysozymesandlauricacid