Cloning of beta-xylosidase gene from aspergillus niger
Filamentous fungi are known to be efficient producers of xylanolytic enzymes, and most commercial xylanolytic preparations are obtained from fermentations of Aspergillus or Trichoderma species. β-xylosidase genes from Aspergillus niger was amplified using Polymerase Chain Reaction. β-xylosidase ge...
| Main Authors: | , |
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| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2007
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| Subjects: | |
| Online Access: | http://eprints.utm.my/5533/1/NorFadzilahMuhammad2007_CloningOfBeta-xylosidaseGene.pdf |
| Summary: | Filamentous fungi are known to be efficient producers of xylanolytic enzymes, and most commercial xylanolytic preparations are obtained from fermentations of Aspergillus or Trichoderma species. β-xylosidase genes from Aspergillus niger was amplified using Polymerase Chain Reaction. β-xylosidase gene in Aspergillus niger contains an open reading frame of 2,412 nucleotides which show high similarity to Aspergillus awamori xyl mRNA for β-xylosidase (Accession Number: AB 154359) . The gene encoded 804 amino acid sequence of β-xylosidase. Amino acid analysis shows 99% identities to β-xylosidase from Aspergillus awamori. |
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