Optimization of soxhlet extraction parameter of annona muricata leaves using box-behnken design (BBD) expert and antioxidant analysis
Response surface methodology (RSM) was employed to optimize the extraction of herbal plant of Annona muricata L. using Box-Behnken Design (BBD). The Box-Behnken Design (BBD) with three factors and two responses (yield and total solid content) was implemented. The processing parameters of A. muricata...
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| Format: | Article |
| Language: | English |
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Penerbit UTM Press
2015
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| Online Access: | http://eprints.utm.my/56200/1/HarisunYa%27akob2015_OptimizationofSoxhletExtractionParameterofAnnona.pdf |
| _version_ | 1796860342376071168 |
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| author | Nik Mat Daud, Nik Nurul Najihah Mohamad Rosdi, Mohamad Norisham Ya’akob, Harisun Musa, Nur Fashya |
| author_facet | Nik Mat Daud, Nik Nurul Najihah Mohamad Rosdi, Mohamad Norisham Ya’akob, Harisun Musa, Nur Fashya |
| author_sort | Nik Mat Daud, Nik Nurul Najihah |
| collection | ePrints |
| description | Response surface methodology (RSM) was employed to optimize the extraction of herbal plant of Annona muricata L. using Box-Behnken Design (BBD). The Box-Behnken Design (BBD) with three factors and two responses (yield and total solid content) was implemented. The processing parameters of A. muricata leaves by soxhlet extraction were solvent to raw material ratio, ethanol concentration, and duration of extraction using soxhlet extractor. The presence of acetogenins compounds was screened by High Performance Liquid Chromatography. Optimum condition with the solvent to raw material ratio (1:5.92), ethanol concentration (1.67 %), and duration of extraction (6.84 hours) was obtained and further analyzed with antioxidant tests. Analyses showed the A. muricata leaves contained high total phenolic and flavonoid contents which corresponding to the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity test. The DPPH result showed that the extract has the highest percentage of scavenging activity with the inhibitory concentration (IC50) value of leaves is 0.243 mg/mL ± 0.001 nearly comparable to butylated hydroxyanisole (BHA) and ascorbic acid (Vit. C), which indicated that the leaves also have higher free radical scavenging activity compared to these commercial standards. Besides, the presence of primary; polysacharide (21.85 % ± 0.001), protein (19.59 % ± 0.000), glycosaponin (18.87 % ± 0.001) and secondary; phenolic (47.26 mg GAE/1g ± 0.001), flavonoid (22.12 mg CE/1g ± 0.001) metabolites in A. muricata leaves suggested that the leaves contained strong antioxidant properties that are believed can act as anticancer agent for the body. |
| first_indexed | 2024-03-05T19:39:56Z |
| format | Article |
| id | utm.eprints-56200 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T19:39:56Z |
| publishDate | 2015 |
| publisher | Penerbit UTM Press |
| record_format | dspace |
| spelling | utm.eprints-562002017-11-01T04:16:48Z http://eprints.utm.my/56200/ Optimization of soxhlet extraction parameter of annona muricata leaves using box-behnken design (BBD) expert and antioxidant analysis Nik Mat Daud, Nik Nurul Najihah Mohamad Rosdi, Mohamad Norisham Ya’akob, Harisun Musa, Nur Fashya R Medicine (General) Response surface methodology (RSM) was employed to optimize the extraction of herbal plant of Annona muricata L. using Box-Behnken Design (BBD). The Box-Behnken Design (BBD) with three factors and two responses (yield and total solid content) was implemented. The processing parameters of A. muricata leaves by soxhlet extraction were solvent to raw material ratio, ethanol concentration, and duration of extraction using soxhlet extractor. The presence of acetogenins compounds was screened by High Performance Liquid Chromatography. Optimum condition with the solvent to raw material ratio (1:5.92), ethanol concentration (1.67 %), and duration of extraction (6.84 hours) was obtained and further analyzed with antioxidant tests. Analyses showed the A. muricata leaves contained high total phenolic and flavonoid contents which corresponding to the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity test. The DPPH result showed that the extract has the highest percentage of scavenging activity with the inhibitory concentration (IC50) value of leaves is 0.243 mg/mL ± 0.001 nearly comparable to butylated hydroxyanisole (BHA) and ascorbic acid (Vit. C), which indicated that the leaves also have higher free radical scavenging activity compared to these commercial standards. Besides, the presence of primary; polysacharide (21.85 % ± 0.001), protein (19.59 % ± 0.000), glycosaponin (18.87 % ± 0.001) and secondary; phenolic (47.26 mg GAE/1g ± 0.001), flavonoid (22.12 mg CE/1g ± 0.001) metabolites in A. muricata leaves suggested that the leaves contained strong antioxidant properties that are believed can act as anticancer agent for the body. Penerbit UTM Press 2015-08 Article PeerReviewed application/pdf en http://eprints.utm.my/56200/1/HarisunYa%27akob2015_OptimizationofSoxhletExtractionParameterofAnnona.pdf Nik Mat Daud, Nik Nurul Najihah and Mohamad Rosdi, Mohamad Norisham and Ya’akob, Harisun and Musa, Nur Fashya (2015) Optimization of soxhlet extraction parameter of annona muricata leaves using box-behnken design (BBD) expert and antioxidant analysis. Jurnal Teknologi, 77 (3). pp. 27-37. ISSN 2180-3722 http://dx.doi.org/10.11113/jt.v77.6001 DOI:10.11113/jt.v77.6001 |
| spellingShingle | R Medicine (General) Nik Mat Daud, Nik Nurul Najihah Mohamad Rosdi, Mohamad Norisham Ya’akob, Harisun Musa, Nur Fashya Optimization of soxhlet extraction parameter of annona muricata leaves using box-behnken design (BBD) expert and antioxidant analysis |
| title | Optimization of soxhlet extraction parameter of annona muricata leaves using box-behnken design (BBD) expert and antioxidant analysis |
| title_full | Optimization of soxhlet extraction parameter of annona muricata leaves using box-behnken design (BBD) expert and antioxidant analysis |
| title_fullStr | Optimization of soxhlet extraction parameter of annona muricata leaves using box-behnken design (BBD) expert and antioxidant analysis |
| title_full_unstemmed | Optimization of soxhlet extraction parameter of annona muricata leaves using box-behnken design (BBD) expert and antioxidant analysis |
| title_short | Optimization of soxhlet extraction parameter of annona muricata leaves using box-behnken design (BBD) expert and antioxidant analysis |
| title_sort | optimization of soxhlet extraction parameter of annona muricata leaves using box behnken design bbd expert and antioxidant analysis |
| topic | R Medicine (General) |
| url | http://eprints.utm.my/56200/1/HarisunYa%27akob2015_OptimizationofSoxhletExtractionParameterofAnnona.pdf |
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