Candida rugosa lipase immobilized onto acid-functionalized multi-walled carbon nanotubes for sustainable production of methyl oleate
The chemical production of methyl oleate using chemically synthesized fatty acid alcohols and other toxic chemicals may lead to significant environmental hazards to mankind. Being a highly valuable fatty acid replacement raw material in oleochemical industry, the mass production of methyl oleate via...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Published: |
Humana Press Inc.
2015
|
| Subjects: |
| _version_ | 1796860420347133952 |
|---|---|
| author | Che Marzuki, Nur Haziqah Mahat, Naji Arafat Huyop, Fahrul Buang, Nor Aziah Abdul Wahab, Roswanira |
| author_facet | Che Marzuki, Nur Haziqah Mahat, Naji Arafat Huyop, Fahrul Buang, Nor Aziah Abdul Wahab, Roswanira |
| author_sort | Che Marzuki, Nur Haziqah |
| collection | ePrints |
| description | The chemical production of methyl oleate using chemically synthesized fatty acid alcohols and other toxic chemicals may lead to significant environmental hazards to mankind. Being a highly valuable fatty acid replacement raw material in oleochemical industry, the mass production of methyl oleate via environmentally favorable processes is of concern. In this context, an alternative technique utilizing Candida rugosa lipase (CRL) physically adsorbed on multi-walled carbon nanotubes (MWCNTs) has been suggested. In this study, the acid-functionalized MWCNTs prepared using a mixture of HNO3 and H2SO4 (1:3 v/v) was used as support for immobilizing CRL onto MWCNTs (CRL–MWCNTs) as biocatalysts. Enzymatic esterification was performed and the efficiency of CRL–MWCNTs was evaluated against the free CRL under varying conditions, viz. temperature, molar ratio of acid/alcohol, solvent log P, and enzyme loading. The CRL–MWCNTs resulted in 30–110 % improvement in the production of methyl oleate over the free CRL. The CRL–MWCNTs attained its highest yield (84.17 %) at 50 °C, molar ratio of acid/alcohol of 1:3, 3 mg/mL of enzyme loading, and iso-octane (log P 4.5) as solvent. Consequently, physical adsorption of CRL onto acid-functionalized MWCNTs has improved the activity and stability of CRL and hence provides an environmentally friendly means for the production of methyl oleate. |
| first_indexed | 2024-03-05T19:41:06Z |
| format | Article |
| id | utm.eprints-57993 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:41:06Z |
| publishDate | 2015 |
| publisher | Humana Press Inc. |
| record_format | dspace |
| spelling | utm.eprints-579932021-12-14T07:41:23Z http://eprints.utm.my/57993/ Candida rugosa lipase immobilized onto acid-functionalized multi-walled carbon nanotubes for sustainable production of methyl oleate Che Marzuki, Nur Haziqah Mahat, Naji Arafat Huyop, Fahrul Buang, Nor Aziah Abdul Wahab, Roswanira QD Chemistry The chemical production of methyl oleate using chemically synthesized fatty acid alcohols and other toxic chemicals may lead to significant environmental hazards to mankind. Being a highly valuable fatty acid replacement raw material in oleochemical industry, the mass production of methyl oleate via environmentally favorable processes is of concern. In this context, an alternative technique utilizing Candida rugosa lipase (CRL) physically adsorbed on multi-walled carbon nanotubes (MWCNTs) has been suggested. In this study, the acid-functionalized MWCNTs prepared using a mixture of HNO3 and H2SO4 (1:3 v/v) was used as support for immobilizing CRL onto MWCNTs (CRL–MWCNTs) as biocatalysts. Enzymatic esterification was performed and the efficiency of CRL–MWCNTs was evaluated against the free CRL under varying conditions, viz. temperature, molar ratio of acid/alcohol, solvent log P, and enzyme loading. The CRL–MWCNTs resulted in 30–110 % improvement in the production of methyl oleate over the free CRL. The CRL–MWCNTs attained its highest yield (84.17 %) at 50 °C, molar ratio of acid/alcohol of 1:3, 3 mg/mL of enzyme loading, and iso-octane (log P 4.5) as solvent. Consequently, physical adsorption of CRL onto acid-functionalized MWCNTs has improved the activity and stability of CRL and hence provides an environmentally friendly means for the production of methyl oleate. Humana Press Inc. 2015 Article PeerReviewed Che Marzuki, Nur Haziqah and Mahat, Naji Arafat and Huyop, Fahrul and Buang, Nor Aziah and Abdul Wahab, Roswanira (2015) Candida rugosa lipase immobilized onto acid-functionalized multi-walled carbon nanotubes for sustainable production of methyl oleate. Applied Biochemistry And Biotechnology, 177 (4). pp. 967-984. ISSN 0273-2289 http://dx.doi.org/10.1007/s12010-015-1791-z DOI:10.1007/s12010-015-1791-z |
| spellingShingle | QD Chemistry Che Marzuki, Nur Haziqah Mahat, Naji Arafat Huyop, Fahrul Buang, Nor Aziah Abdul Wahab, Roswanira Candida rugosa lipase immobilized onto acid-functionalized multi-walled carbon nanotubes for sustainable production of methyl oleate |
| title | Candida rugosa lipase immobilized onto acid-functionalized multi-walled carbon nanotubes for sustainable production of methyl oleate |
| title_full | Candida rugosa lipase immobilized onto acid-functionalized multi-walled carbon nanotubes for sustainable production of methyl oleate |
| title_fullStr | Candida rugosa lipase immobilized onto acid-functionalized multi-walled carbon nanotubes for sustainable production of methyl oleate |
| title_full_unstemmed | Candida rugosa lipase immobilized onto acid-functionalized multi-walled carbon nanotubes for sustainable production of methyl oleate |
| title_short | Candida rugosa lipase immobilized onto acid-functionalized multi-walled carbon nanotubes for sustainable production of methyl oleate |
| title_sort | candida rugosa lipase immobilized onto acid functionalized multi walled carbon nanotubes for sustainable production of methyl oleate |
| topic | QD Chemistry |
| work_keys_str_mv | AT chemarzukinurhaziqah candidarugosalipaseimmobilizedontoacidfunctionalizedmultiwalledcarbonnanotubesforsustainableproductionofmethyloleate AT mahatnajiarafat candidarugosalipaseimmobilizedontoacidfunctionalizedmultiwalledcarbonnanotubesforsustainableproductionofmethyloleate AT huyopfahrul candidarugosalipaseimmobilizedontoacidfunctionalizedmultiwalledcarbonnanotubesforsustainableproductionofmethyloleate AT buangnoraziah candidarugosalipaseimmobilizedontoacidfunctionalizedmultiwalledcarbonnanotubesforsustainableproductionofmethyloleate AT abdulwahabroswanira candidarugosalipaseimmobilizedontoacidfunctionalizedmultiwalledcarbonnanotubesforsustainableproductionofmethyloleate |