Chiral separation of four stereoisomers of ketoconazole drugs using capillary electrophoresis
This work aimed to develop a chiral separation method of ketoconazole enantiomers using electrokinetic chromatography. The separation was achieved using heptakis (2, 3, 6-tri-O-methyl)-β-cyclodextrin (TMβCD), a commonly used chiral selector (CS), as it is relatively inexpensive and has a low UV abso...
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| Format: | Article |
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John Wiley and Sons Inc.
2015
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| _version_ | 1796860431024783360 |
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| author | Wan Ibrahim, Wan Aini Arsad, Siti Rosilah Maarof, Hasmerya Sanagi, Mohd. Marsin Aboul Enein, Hassan Y. |
| author_facet | Wan Ibrahim, Wan Aini Arsad, Siti Rosilah Maarof, Hasmerya Sanagi, Mohd. Marsin Aboul Enein, Hassan Y. |
| author_sort | Wan Ibrahim, Wan Aini |
| collection | ePrints |
| description | This work aimed to develop a chiral separation method of ketoconazole enantiomers using electrokinetic chromatography. The separation was achieved using heptakis (2, 3, 6-tri-O-methyl)-β-cyclodextrin (TMβCD), a commonly used chiral selector (CS), as it is relatively inexpensive and has a low UV absorbance in addition to an anionic surfactant, sodium dodecyl sulfate (SDS). The influence of TMβCD concentration, phosphate buffer concentration, SDS concentration, buffer pH, and applied voltage were investigated. The optimum conditions for chiral separation of ketoconazole was achieved using 10 mM phosphate buffer at pH 2.5 containing 20 mM TMβCD, 5 mM SDS, and 1.0% (v/v) methanol with an applied voltage of 25 kV at 25 °C with a 5-s injection time (hydrodynamic injection). The four ketoconazole stereoisomers were successfully resolved for the first time within 17 min (total analysis time was 28 min including capillary conditioning). The migration time precision of this method was examined to give repeatability and reproducibility with RSDs ≤5.80% (n =3) and RSDs ≤8.88% (n =9), respectively. |
| first_indexed | 2024-03-05T19:41:15Z |
| format | Article |
| id | utm.eprints-58046 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:41:15Z |
| publishDate | 2015 |
| publisher | John Wiley and Sons Inc. |
| record_format | dspace |
| spelling | utm.eprints-580462021-09-14T05:39:34Z http://eprints.utm.my/58046/ Chiral separation of four stereoisomers of ketoconazole drugs using capillary electrophoresis Wan Ibrahim, Wan Aini Arsad, Siti Rosilah Maarof, Hasmerya Sanagi, Mohd. Marsin Aboul Enein, Hassan Y. QD Chemistry This work aimed to develop a chiral separation method of ketoconazole enantiomers using electrokinetic chromatography. The separation was achieved using heptakis (2, 3, 6-tri-O-methyl)-β-cyclodextrin (TMβCD), a commonly used chiral selector (CS), as it is relatively inexpensive and has a low UV absorbance in addition to an anionic surfactant, sodium dodecyl sulfate (SDS). The influence of TMβCD concentration, phosphate buffer concentration, SDS concentration, buffer pH, and applied voltage were investigated. The optimum conditions for chiral separation of ketoconazole was achieved using 10 mM phosphate buffer at pH 2.5 containing 20 mM TMβCD, 5 mM SDS, and 1.0% (v/v) methanol with an applied voltage of 25 kV at 25 °C with a 5-s injection time (hydrodynamic injection). The four ketoconazole stereoisomers were successfully resolved for the first time within 17 min (total analysis time was 28 min including capillary conditioning). The migration time precision of this method was examined to give repeatability and reproducibility with RSDs ≤5.80% (n =3) and RSDs ≤8.88% (n =9), respectively. John Wiley and Sons Inc. 2015-03 Article PeerReviewed Wan Ibrahim, Wan Aini and Arsad, Siti Rosilah and Maarof, Hasmerya and Sanagi, Mohd. Marsin and Aboul Enein, Hassan Y. (2015) Chiral separation of four stereoisomers of ketoconazole drugs using capillary electrophoresis. Chirality, 27 (3). pp. 223-227. ISSN 0899-0042 http://dx.doi.org/10.1002/chir.22416 DOI:10.1002/chir.22416 |
| spellingShingle | QD Chemistry Wan Ibrahim, Wan Aini Arsad, Siti Rosilah Maarof, Hasmerya Sanagi, Mohd. Marsin Aboul Enein, Hassan Y. Chiral separation of four stereoisomers of ketoconazole drugs using capillary electrophoresis |
| title | Chiral separation of four stereoisomers of ketoconazole drugs using capillary electrophoresis |
| title_full | Chiral separation of four stereoisomers of ketoconazole drugs using capillary electrophoresis |
| title_fullStr | Chiral separation of four stereoisomers of ketoconazole drugs using capillary electrophoresis |
| title_full_unstemmed | Chiral separation of four stereoisomers of ketoconazole drugs using capillary electrophoresis |
| title_short | Chiral separation of four stereoisomers of ketoconazole drugs using capillary electrophoresis |
| title_sort | chiral separation of four stereoisomers of ketoconazole drugs using capillary electrophoresis |
| topic | QD Chemistry |
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