Cyclodextrin glycosyltransferase biosynthesis improvement by recombinant Lactococcus lactis NZ:NSP:CGT: medium formulation and culture condition optimization
Cyclodextrin glycosyltransferase (CGTase) is a bacterial glycosyltransferase which catalyses the conversion of starch to cyclodextrin. It can be produced industrially through a fermentation process. The optimal design of the fermentation medium and conditions is critical for metabolic production and...
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| Format: | Article |
| Language: | English |
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Diagnosis Press Limited
2015
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| Online Access: | http://eprints.utm.my/58199/1/RosliMdIllias2015_CyclodextrinGlycosyltransferaseBiosynthesisImprovement.pdf |
| _version_ | 1796860461408321536 |
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| author | Amiri, Azin Mohamad, Rosfarizan Abdul Rahim, Raha Md. Illias, Rosli Namvar, Farideh Tan, Joo Shun Abbasiliasi, Sahar |
| author_facet | Amiri, Azin Mohamad, Rosfarizan Abdul Rahim, Raha Md. Illias, Rosli Namvar, Farideh Tan, Joo Shun Abbasiliasi, Sahar |
| author_sort | Amiri, Azin |
| collection | ePrints |
| description | Cyclodextrin glycosyltransferase (CGTase) is a bacterial glycosyltransferase which catalyses the conversion of starch to cyclodextrin. It can be produced industrially through a fermentation process. The optimal design of the fermentation medium and conditions is critical for metabolic production and microbial growth. Optimization of CGTase biosynthesis by recombinant Lactococcus lactis NZ:NSP:CGT was performed using an artificial neural network (ANN) as the main tool in order to improve the CGTase production in the fermentation process. Three parameters, including temperature, carbon source concentration and nitrogen source concentration, were determined as significant factors for improvement of CGTase production by L. lactis NZ:NSP:CGT in the fermentation process. Soluble potato starch and yeast extract were chosen as the best carbon and nitrogen sources for higher production of CGTase by L. lactis NZ:NSP:CGT. The optimum concentration of soluble starch and yeast extract were 3.82% and 5.67% (w/v), respectively, and the optimum temperature was 20 °C. The final CGTase activity under optimized conditions was 22.09 U/mL, which was close enough to the predicted value of 24.17 U/mL. The obtained results are of particular interest, as CGTase is an industrially important enzyme. In this study, its production through batch fermentation was optimized in order to maximize its production. In future studies, more investigations in various modes of fermentation should be conducted in order to increase the CGTase biosynthesis. |
| first_indexed | 2024-03-05T19:41:43Z |
| format | Article |
| id | utm.eprints-58199 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T19:41:43Z |
| publishDate | 2015 |
| publisher | Diagnosis Press Limited |
| record_format | dspace |
| spelling | utm.eprints-581992021-12-07T01:17:47Z http://eprints.utm.my/58199/ Cyclodextrin glycosyltransferase biosynthesis improvement by recombinant Lactococcus lactis NZ:NSP:CGT: medium formulation and culture condition optimization Amiri, Azin Mohamad, Rosfarizan Abdul Rahim, Raha Md. Illias, Rosli Namvar, Farideh Tan, Joo Shun Abbasiliasi, Sahar TP Chemical technology Cyclodextrin glycosyltransferase (CGTase) is a bacterial glycosyltransferase which catalyses the conversion of starch to cyclodextrin. It can be produced industrially through a fermentation process. The optimal design of the fermentation medium and conditions is critical for metabolic production and microbial growth. Optimization of CGTase biosynthesis by recombinant Lactococcus lactis NZ:NSP:CGT was performed using an artificial neural network (ANN) as the main tool in order to improve the CGTase production in the fermentation process. Three parameters, including temperature, carbon source concentration and nitrogen source concentration, were determined as significant factors for improvement of CGTase production by L. lactis NZ:NSP:CGT in the fermentation process. Soluble potato starch and yeast extract were chosen as the best carbon and nitrogen sources for higher production of CGTase by L. lactis NZ:NSP:CGT. The optimum concentration of soluble starch and yeast extract were 3.82% and 5.67% (w/v), respectively, and the optimum temperature was 20 °C. The final CGTase activity under optimized conditions was 22.09 U/mL, which was close enough to the predicted value of 24.17 U/mL. The obtained results are of particular interest, as CGTase is an industrially important enzyme. In this study, its production through batch fermentation was optimized in order to maximize its production. In future studies, more investigations in various modes of fermentation should be conducted in order to increase the CGTase biosynthesis. Diagnosis Press Limited 2015 Article PeerReviewed application/pdf en http://eprints.utm.my/58199/1/RosliMdIllias2015_CyclodextrinGlycosyltransferaseBiosynthesisImprovement.pdf Amiri, Azin and Mohamad, Rosfarizan and Abdul Rahim, Raha and Md. Illias, Rosli and Namvar, Farideh and Tan, Joo Shun and Abbasiliasi, Sahar (2015) Cyclodextrin glycosyltransferase biosynthesis improvement by recombinant Lactococcus lactis NZ:NSP:CGT: medium formulation and culture condition optimization. Biotechnology & Biotechnological Equipment, 29 (3). pp. 555-563. ISSN 1310-2818 http://dx.doi.org/10.1080/13102818.2015.1009713 DOI:10.1080/13102818.2015.1009713 |
| spellingShingle | TP Chemical technology Amiri, Azin Mohamad, Rosfarizan Abdul Rahim, Raha Md. Illias, Rosli Namvar, Farideh Tan, Joo Shun Abbasiliasi, Sahar Cyclodextrin glycosyltransferase biosynthesis improvement by recombinant Lactococcus lactis NZ:NSP:CGT: medium formulation and culture condition optimization |
| title | Cyclodextrin glycosyltransferase biosynthesis improvement by recombinant Lactococcus lactis NZ:NSP:CGT: medium formulation and culture condition optimization |
| title_full | Cyclodextrin glycosyltransferase biosynthesis improvement by recombinant Lactococcus lactis NZ:NSP:CGT: medium formulation and culture condition optimization |
| title_fullStr | Cyclodextrin glycosyltransferase biosynthesis improvement by recombinant Lactococcus lactis NZ:NSP:CGT: medium formulation and culture condition optimization |
| title_full_unstemmed | Cyclodextrin glycosyltransferase biosynthesis improvement by recombinant Lactococcus lactis NZ:NSP:CGT: medium formulation and culture condition optimization |
| title_short | Cyclodextrin glycosyltransferase biosynthesis improvement by recombinant Lactococcus lactis NZ:NSP:CGT: medium formulation and culture condition optimization |
| title_sort | cyclodextrin glycosyltransferase biosynthesis improvement by recombinant lactococcus lactis nz nsp cgt medium formulation and culture condition optimization |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/58199/1/RosliMdIllias2015_CyclodextrinGlycosyltransferaseBiosynthesisImprovement.pdf |
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