Differentiating the extent of cartilage repair in rabbit ears using nonlinear optical microscopy
Nonlinear optical microscopy (NLOM) was used as a noninvasive and label-free tool to detect and quantify the extent of the cartilage recovery. Two cartilage injury models were established in the outer ears of rabbits that created a different extent of cartilage recovery based on the presence or abse...
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| Format: | Article |
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Blackwell Publishing Ltd
2015
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| author | Zhu, X. Q. Xu, Y. H. Liao, C. X. Liu, W. G. Cheng, K. K. Chen, J. |
| author_facet | Zhu, X. Q. Xu, Y. H. Liao, C. X. Liu, W. G. Cheng, K. K. Chen, J. |
| author_sort | Zhu, X. Q. |
| collection | ePrints |
| description | Nonlinear optical microscopy (NLOM) was used as a noninvasive and label-free tool to detect and quantify the extent of the cartilage recovery. Two cartilage injury models were established in the outer ears of rabbits that created a different extent of cartilage recovery based on the presence or absence of the perichondrium. High-resolution NLOM images were used to measure cartilage repair, specifically through spectral analysis and image texture. In contrast to a wound lacking a perichondrium, wounds with intact perichondria demonstrated significantly larger TPEF signals from cells and matrix, coarser texture indicating the more deposition of type I collagen. Spectral analysis of cells and matrix can reveal the matrix properties and cell growth. In addition, texture analysis of NLOM images showed significant differences in the distribution of cells and matrix of repaired tissues with or without perichondrium. Specifically, the decay length of autocorrelation coefficient based on TPEF images is 11.2 ± 1.1 in Wound 2 (with perichondrium) and 7.5 ± 2.0 in Wound 1 (without perichondrium), indicating coarser image texture and faster growth of cells in repaired tissues with perichondrium (p < 0.05). Moreover, the decay length of autocorrelation coefficient based on collagen SHG images also showed significant difference between Wound 2 and 1 (16.2 ± 1.2 vs. 12.2 ± 2.1, p < 0.05), indicating coarser image texture and faster deposition of collagen in repaired tissues with perichondrium (Wound 2). These findings suggest that NLOM is an ideal tool for studying cartilage repair, with potential applications in clinical medicine. NLOM can capture macromolecular details and distinguish between different extents of cartilage repair without the need for labelling agents. |
| first_indexed | 2024-03-05T19:42:02Z |
| format | Article |
| id | utm.eprints-58310 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:42:02Z |
| publishDate | 2015 |
| publisher | Blackwell Publishing Ltd |
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| spelling | utm.eprints-583102021-12-21T01:57:57Z http://eprints.utm.my/58310/ Differentiating the extent of cartilage repair in rabbit ears using nonlinear optical microscopy Zhu, X. Q. Xu, Y. H. Liao, C. X. Liu, W. G. Cheng, K. K. Chen, J. QH Natural history Nonlinear optical microscopy (NLOM) was used as a noninvasive and label-free tool to detect and quantify the extent of the cartilage recovery. Two cartilage injury models were established in the outer ears of rabbits that created a different extent of cartilage recovery based on the presence or absence of the perichondrium. High-resolution NLOM images were used to measure cartilage repair, specifically through spectral analysis and image texture. In contrast to a wound lacking a perichondrium, wounds with intact perichondria demonstrated significantly larger TPEF signals from cells and matrix, coarser texture indicating the more deposition of type I collagen. Spectral analysis of cells and matrix can reveal the matrix properties and cell growth. In addition, texture analysis of NLOM images showed significant differences in the distribution of cells and matrix of repaired tissues with or without perichondrium. Specifically, the decay length of autocorrelation coefficient based on TPEF images is 11.2 ± 1.1 in Wound 2 (with perichondrium) and 7.5 ± 2.0 in Wound 1 (without perichondrium), indicating coarser image texture and faster growth of cells in repaired tissues with perichondrium (p < 0.05). Moreover, the decay length of autocorrelation coefficient based on collagen SHG images also showed significant difference between Wound 2 and 1 (16.2 ± 1.2 vs. 12.2 ± 2.1, p < 0.05), indicating coarser image texture and faster deposition of collagen in repaired tissues with perichondrium (Wound 2). These findings suggest that NLOM is an ideal tool for studying cartilage repair, with potential applications in clinical medicine. NLOM can capture macromolecular details and distinguish between different extents of cartilage repair without the need for labelling agents. Blackwell Publishing Ltd 2015-11 Article PeerReviewed Zhu, X. Q. and Xu, Y. H. and Liao, C. X. and Liu, W. G. and Cheng, K. K. and Chen, J. (2015) Differentiating the extent of cartilage repair in rabbit ears using nonlinear optical microscopy. Journal of Microscopy, 260 (2). pp. 219-226. ISSN 0022-2720 http://dx.doi.org/10.1111/jmi.12288 DOI:10.1111/jmi.12288 |
| spellingShingle | QH Natural history Zhu, X. Q. Xu, Y. H. Liao, C. X. Liu, W. G. Cheng, K. K. Chen, J. Differentiating the extent of cartilage repair in rabbit ears using nonlinear optical microscopy |
| title | Differentiating the extent of cartilage repair in rabbit ears using nonlinear optical microscopy |
| title_full | Differentiating the extent of cartilage repair in rabbit ears using nonlinear optical microscopy |
| title_fullStr | Differentiating the extent of cartilage repair in rabbit ears using nonlinear optical microscopy |
| title_full_unstemmed | Differentiating the extent of cartilage repair in rabbit ears using nonlinear optical microscopy |
| title_short | Differentiating the extent of cartilage repair in rabbit ears using nonlinear optical microscopy |
| title_sort | differentiating the extent of cartilage repair in rabbit ears using nonlinear optical microscopy |
| topic | QH Natural history |
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