Dispersive micro-solid phase extraction combined with high-performance liquid chromatography for the determination of three penicillins in milk samples
A simple, rapid, and environmentally friendly microextraction termed COU-2-D-μ-SPE has been developed for the analysis of three penicillins (i.e., oxacillin, cloxacillin, and dicloxacillin) in milk samples. An ordered mesoporous carbon, COU-2, was synthesized and used as the sorbent in dispersive mi...
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Springer New York LLC
2015
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| author | Yahaya, Noorfatimah Sanagi, Mohd. Marsin Mitome, Takahito Nishiyama, Norikazu Wan Ibrahim, Wan Aini Nur, Hadi |
| author_facet | Yahaya, Noorfatimah Sanagi, Mohd. Marsin Mitome, Takahito Nishiyama, Norikazu Wan Ibrahim, Wan Aini Nur, Hadi |
| author_sort | Yahaya, Noorfatimah |
| collection | ePrints |
| description | A simple, rapid, and environmentally friendly microextraction termed COU-2-D-μ-SPE has been developed for the analysis of three penicillins (i.e., oxacillin, cloxacillin, and dicloxacillin) in milk samples. An ordered mesoporous carbon, COU-2, was synthesized and used as the sorbent in dispersive micro-solid phase for the extraction of selected penicillins prior to high-performance liquid chromatography-ultraviolet detection (HPLC-UV). Several important parameters, namely, pH value, salt addition, desorption solvent, extraction time, desorption time, and amount of COU-2, were investigated and optimized. Under the optimum extraction conditions, the method showed good linearity in the range of 10–5,000 μg L−1 (r2 ≥ 0.9994), low limits of detection (2.0–3.3 μg L−1), acceptable reproducibility (relative standard deviation (RSD) 6.2–8.8 %, n = 9), and satisfactory relative recoveries (80.3–99.5 %) for studied penicillins in milk. The proposed COU-2-D-μ-SPE method has been successfully applied to six commercial milk samples, and the extraction of blank samples indicated that all samples were free from selected penicillin contamination. |
| first_indexed | 2024-03-05T19:42:04Z |
| format | Article |
| id | utm.eprints-58319 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:42:04Z |
| publishDate | 2015 |
| publisher | Springer New York LLC |
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| spelling | utm.eprints-583192021-12-13T02:35:06Z http://eprints.utm.my/58319/ Dispersive micro-solid phase extraction combined with high-performance liquid chromatography for the determination of three penicillins in milk samples Yahaya, Noorfatimah Sanagi, Mohd. Marsin Mitome, Takahito Nishiyama, Norikazu Wan Ibrahim, Wan Aini Nur, Hadi QD Chemistry A simple, rapid, and environmentally friendly microextraction termed COU-2-D-μ-SPE has been developed for the analysis of three penicillins (i.e., oxacillin, cloxacillin, and dicloxacillin) in milk samples. An ordered mesoporous carbon, COU-2, was synthesized and used as the sorbent in dispersive micro-solid phase for the extraction of selected penicillins prior to high-performance liquid chromatography-ultraviolet detection (HPLC-UV). Several important parameters, namely, pH value, salt addition, desorption solvent, extraction time, desorption time, and amount of COU-2, were investigated and optimized. Under the optimum extraction conditions, the method showed good linearity in the range of 10–5,000 μg L−1 (r2 ≥ 0.9994), low limits of detection (2.0–3.3 μg L−1), acceptable reproducibility (relative standard deviation (RSD) 6.2–8.8 %, n = 9), and satisfactory relative recoveries (80.3–99.5 %) for studied penicillins in milk. The proposed COU-2-D-μ-SPE method has been successfully applied to six commercial milk samples, and the extraction of blank samples indicated that all samples were free from selected penicillin contamination. Springer New York LLC 2015-04-10 Article PeerReviewed Yahaya, Noorfatimah and Sanagi, Mohd. Marsin and Mitome, Takahito and Nishiyama, Norikazu and Wan Ibrahim, Wan Aini and Nur, Hadi (2015) Dispersive micro-solid phase extraction combined with high-performance liquid chromatography for the determination of three penicillins in milk samples. Food Alytical Methods, 8 (5). pp. 1079-1087. ISSN 1936-9751 http://dx.doi.org/10.1007/s12161-014-9991-7 DOI:10.1007/s12161-014-9991-7 |
| spellingShingle | QD Chemistry Yahaya, Noorfatimah Sanagi, Mohd. Marsin Mitome, Takahito Nishiyama, Norikazu Wan Ibrahim, Wan Aini Nur, Hadi Dispersive micro-solid phase extraction combined with high-performance liquid chromatography for the determination of three penicillins in milk samples |
| title | Dispersive micro-solid phase extraction combined with high-performance liquid chromatography for the determination of three penicillins in milk samples |
| title_full | Dispersive micro-solid phase extraction combined with high-performance liquid chromatography for the determination of three penicillins in milk samples |
| title_fullStr | Dispersive micro-solid phase extraction combined with high-performance liquid chromatography for the determination of three penicillins in milk samples |
| title_full_unstemmed | Dispersive micro-solid phase extraction combined with high-performance liquid chromatography for the determination of three penicillins in milk samples |
| title_short | Dispersive micro-solid phase extraction combined with high-performance liquid chromatography for the determination of three penicillins in milk samples |
| title_sort | dispersive micro solid phase extraction combined with high performance liquid chromatography for the determination of three penicillins in milk samples |
| topic | QD Chemistry |
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