Liquid chromatographic determination of NSAIDs in urine after dispersive liquid-liquid microextraction based on solidification of floating organic droplets
An environmentally benign method of sample preparation based on dispersive liquid–liquid microextraction and solidification of floating organic droplets (DLLME-SFO) coupled with high-performance liquid chromatography with ultraviolet detection has been developed for analysis of non-steroidal anti-in...
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| Format: | Article |
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Friedr. Vieweg und Sohn Verlags GmbH
2015
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| author | Md. Shukri, Dyia Syaleyana Sanagi, Mohd. Marsin Wan Ibrahim, Wan Aini Zainal Abidin, Nurul Nabilah Aboul-Enein, Hassan Y. |
| author_facet | Md. Shukri, Dyia Syaleyana Sanagi, Mohd. Marsin Wan Ibrahim, Wan Aini Zainal Abidin, Nurul Nabilah Aboul-Enein, Hassan Y. |
| author_sort | Md. Shukri, Dyia Syaleyana |
| collection | ePrints |
| description | An environmentally benign method of sample preparation based on dispersive liquid–liquid microextraction and solidification of floating organic droplets (DLLME-SFO) coupled with high-performance liquid chromatography with ultraviolet detection has been developed for analysis of non-steroidal anti-inflammatory drugs (NSAIDs) in biological fluids. A low-toxicity solvent was used to replace the chlorinated solvents commonly used in conventional DLLME. Seven conditions were investigated and optimized: type and volume of extraction solvent and dispersive solvent, extraction time, effect of addition of salt, and sample pH. Under the optimum conditions, good linearity was obtained in the range 0.01–10 µg mL−1, with coefficients of determination (r2) >0.9949. Detection limits were in the range 0.0034–0.0052 µg mL−1 with good reproducibility (RSD) and satisfactory inter-day and intra-day recovery (95.7–115.6 %). The method was successfully used for analysis of diclofenac, mefenamic acid, and ketoprofen in human urine. Analysis of urine samples from a patient 2 and 4 h after administration of diclofenac revealed concentrations of 1.20 and 0.34 µg mL−1, respectively. |
| first_indexed | 2024-03-05T19:42:34Z |
| format | Article |
| id | utm.eprints-58486 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:42:34Z |
| publishDate | 2015 |
| publisher | Friedr. Vieweg und Sohn Verlags GmbH |
| record_format | dspace |
| spelling | utm.eprints-584862021-12-15T03:50:23Z http://eprints.utm.my/58486/ Liquid chromatographic determination of NSAIDs in urine after dispersive liquid-liquid microextraction based on solidification of floating organic droplets Md. Shukri, Dyia Syaleyana Sanagi, Mohd. Marsin Wan Ibrahim, Wan Aini Zainal Abidin, Nurul Nabilah Aboul-Enein, Hassan Y. QD Chemistry An environmentally benign method of sample preparation based on dispersive liquid–liquid microextraction and solidification of floating organic droplets (DLLME-SFO) coupled with high-performance liquid chromatography with ultraviolet detection has been developed for analysis of non-steroidal anti-inflammatory drugs (NSAIDs) in biological fluids. A low-toxicity solvent was used to replace the chlorinated solvents commonly used in conventional DLLME. Seven conditions were investigated and optimized: type and volume of extraction solvent and dispersive solvent, extraction time, effect of addition of salt, and sample pH. Under the optimum conditions, good linearity was obtained in the range 0.01–10 µg mL−1, with coefficients of determination (r2) >0.9949. Detection limits were in the range 0.0034–0.0052 µg mL−1 with good reproducibility (RSD) and satisfactory inter-day and intra-day recovery (95.7–115.6 %). The method was successfully used for analysis of diclofenac, mefenamic acid, and ketoprofen in human urine. Analysis of urine samples from a patient 2 and 4 h after administration of diclofenac revealed concentrations of 1.20 and 0.34 µg mL−1, respectively. Friedr. Vieweg und Sohn Verlags GmbH 2015 Article PeerReviewed Md. Shukri, Dyia Syaleyana and Sanagi, Mohd. Marsin and Wan Ibrahim, Wan Aini and Zainal Abidin, Nurul Nabilah and Aboul-Enein, Hassan Y. (2015) Liquid chromatographic determination of NSAIDs in urine after dispersive liquid-liquid microextraction based on solidification of floating organic droplets. Chromatographia, 78 (15-16). pp. 987-994. ISSN 0009-5893 http://dx.doi.org/10.1007/s10337-015-2920-0 |
| spellingShingle | QD Chemistry Md. Shukri, Dyia Syaleyana Sanagi, Mohd. Marsin Wan Ibrahim, Wan Aini Zainal Abidin, Nurul Nabilah Aboul-Enein, Hassan Y. Liquid chromatographic determination of NSAIDs in urine after dispersive liquid-liquid microextraction based on solidification of floating organic droplets |
| title | Liquid chromatographic determination of NSAIDs in urine after dispersive liquid-liquid microextraction based on solidification of floating organic droplets |
| title_full | Liquid chromatographic determination of NSAIDs in urine after dispersive liquid-liquid microextraction based on solidification of floating organic droplets |
| title_fullStr | Liquid chromatographic determination of NSAIDs in urine after dispersive liquid-liquid microextraction based on solidification of floating organic droplets |
| title_full_unstemmed | Liquid chromatographic determination of NSAIDs in urine after dispersive liquid-liquid microextraction based on solidification of floating organic droplets |
| title_short | Liquid chromatographic determination of NSAIDs in urine after dispersive liquid-liquid microextraction based on solidification of floating organic droplets |
| title_sort | liquid chromatographic determination of nsaids in urine after dispersive liquid liquid microextraction based on solidification of floating organic droplets |
| topic | QD Chemistry |
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