Microfluidic vascularized bone tissue model with hydroxyapatite-incorporated extracellular matrix
Current in vitro systems mimicking bone tissues fail to fully integrate the three-dimensional (3D) microvasculature and bone tissue microenvironments, decreasing their similarity to in vivo conditions. Here, we propose 3D microvascular networks in a hydroxyapatite (HA)-incorporated extracellular mat...
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| Format: | Article |
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Royal Society of Chemistry Funding details
2015
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| _version_ | 1796860530016649216 |
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| author | Jusoh, Norhana Oh, Soo Jung Kim, Su Dong Kim, Jang Ho Jeon, Noo li |
| author_facet | Jusoh, Norhana Oh, Soo Jung Kim, Su Dong Kim, Jang Ho Jeon, Noo li |
| author_sort | Jusoh, Norhana |
| collection | ePrints |
| description | Current in vitro systems mimicking bone tissues fail to fully integrate the three-dimensional (3D) microvasculature and bone tissue microenvironments, decreasing their similarity to in vivo conditions. Here, we propose 3D microvascular networks in a hydroxyapatite (HA)-incorporated extracellular matrix (ECM) for designing and manipulating a vascularized bone tissue model in a microfluidic device. Incorporation of HA of various concentrations resulted in ECM with varying mechanical properties. Sprouting angiogenesis was affected by mechanically modulated HA-extracellular matrix interactions, generating a model of vascularized bone microenvironment. Using this platform, we observed that hydroxyapatite enhanced angiogenic properties such as sprout length, sprouting speed, sprout number, and lumen diameter. This new platform integrates fibrin ECM with the synthetic bone mineral HA to provide in vivo-like microenvironments for bone vessel sprouting. |
| first_indexed | 2024-03-05T19:42:44Z |
| format | Article |
| id | utm.eprints-58546 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:42:44Z |
| publishDate | 2015 |
| publisher | Royal Society of Chemistry Funding details |
| record_format | dspace |
| spelling | utm.eprints-585462021-08-04T07:38:13Z http://eprints.utm.my/58546/ Microfluidic vascularized bone tissue model with hydroxyapatite-incorporated extracellular matrix Jusoh, Norhana Oh, Soo Jung Kim, Su Dong Kim, Jang Ho Jeon, Noo li Q Science (General) Current in vitro systems mimicking bone tissues fail to fully integrate the three-dimensional (3D) microvasculature and bone tissue microenvironments, decreasing their similarity to in vivo conditions. Here, we propose 3D microvascular networks in a hydroxyapatite (HA)-incorporated extracellular matrix (ECM) for designing and manipulating a vascularized bone tissue model in a microfluidic device. Incorporation of HA of various concentrations resulted in ECM with varying mechanical properties. Sprouting angiogenesis was affected by mechanically modulated HA-extracellular matrix interactions, generating a model of vascularized bone microenvironment. Using this platform, we observed that hydroxyapatite enhanced angiogenic properties such as sprout length, sprouting speed, sprout number, and lumen diameter. This new platform integrates fibrin ECM with the synthetic bone mineral HA to provide in vivo-like microenvironments for bone vessel sprouting. Royal Society of Chemistry Funding details 2015 Article PeerReviewed Jusoh, Norhana and Oh, Soo Jung and Kim, Su Dong and Kim, Jang Ho and Jeon, Noo li (2015) Microfluidic vascularized bone tissue model with hydroxyapatite-incorporated extracellular matrix. Lab On A Chip - Miniaturisation For Chemistry And Biology, 15 (20). pp. 3984-3988. ISSN 1473-0197 http://dx.doi.org/10.1039/c5lc00698h DOI:10.1039/c5lc00698h |
| spellingShingle | Q Science (General) Jusoh, Norhana Oh, Soo Jung Kim, Su Dong Kim, Jang Ho Jeon, Noo li Microfluidic vascularized bone tissue model with hydroxyapatite-incorporated extracellular matrix |
| title | Microfluidic vascularized bone tissue model with hydroxyapatite-incorporated extracellular matrix |
| title_full | Microfluidic vascularized bone tissue model with hydroxyapatite-incorporated extracellular matrix |
| title_fullStr | Microfluidic vascularized bone tissue model with hydroxyapatite-incorporated extracellular matrix |
| title_full_unstemmed | Microfluidic vascularized bone tissue model with hydroxyapatite-incorporated extracellular matrix |
| title_short | Microfluidic vascularized bone tissue model with hydroxyapatite-incorporated extracellular matrix |
| title_sort | microfluidic vascularized bone tissue model with hydroxyapatite incorporated extracellular matrix |
| topic | Q Science (General) |
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