Identification and characterization of potential useful bacteria from marine environment

Marine environment remained as largely unexplored source for researchers to discover novel properties from marine organisms which can benefits human kind. The study aims to isolate marine bacterium from various source of marine environment. Six bacterial strains were successfully isolated from marin...

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Main Authors: Thevarajoo, Suganthi, Goh, Kian Mau, Abd. Manan, Fazilah, Ibrahim, Zaharah, Chong, Chun Shiong
Format: Conference or Workshop Item
Language:English
Published: 2014
Subjects:
Online Access:http://eprints.utm.my/61038/1/ChongChunShiong2014_IdentificationandCharacterizationofPotentialUsefulBacteria.pdf
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author Thevarajoo, Suganthi
Goh, Kian Mau
Abd. Manan, Fazilah
Ibrahim, Zaharah
Chong, Chun Shiong
author_facet Thevarajoo, Suganthi
Goh, Kian Mau
Abd. Manan, Fazilah
Ibrahim, Zaharah
Chong, Chun Shiong
author_sort Thevarajoo, Suganthi
collection ePrints
description Marine environment remained as largely unexplored source for researchers to discover novel properties from marine organisms which can benefits human kind. The study aims to isolate marine bacterium from various source of marine environment. Six bacterial strains were successfully isolated from marine samples from seashore of the Desaru, Malaysia and identified by 16S rRNA sequencing. The characterizations of bacterial strains were also performed based morphological tests, Gram staining, biochemical tests and antibiotic sensitivity against several antibiotics by disc diffusion method. The 16S rRNA sequence of D-2, D-4, D-7, D-15, D-31 and D-33 revealed a high identity of 97 to 99% with 16S rRNA sequence belong to genera Pseudomonas, Marinomonas, Exiquobacterium,Micrococcus, Pseudoalteromonasand Shewanella respectively. Strain D-31 exhibited higher tolerance towards antibiotic with resistance to Kanamycin, Ampicillin and Erythromycin. However, the growth of other strains was retarded by at least two of the antibiotics on their normal growth. The isolation of marine bacterial strain belongs to Marinomonas sp. and Pseudoalteromonassp. create of interest for further biological characterization as the strains from these two genera have been proven for the discovery of new antimicrobial substances, enzymes for industry application and unique secondary metabolites.
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spelling utm.eprints-610382017-03-12T08:25:19Z http://eprints.utm.my/61038/ Identification and characterization of potential useful bacteria from marine environment Thevarajoo, Suganthi Goh, Kian Mau Abd. Manan, Fazilah Ibrahim, Zaharah Chong, Chun Shiong R Medicine (General) Marine environment remained as largely unexplored source for researchers to discover novel properties from marine organisms which can benefits human kind. The study aims to isolate marine bacterium from various source of marine environment. Six bacterial strains were successfully isolated from marine samples from seashore of the Desaru, Malaysia and identified by 16S rRNA sequencing. The characterizations of bacterial strains were also performed based morphological tests, Gram staining, biochemical tests and antibiotic sensitivity against several antibiotics by disc diffusion method. The 16S rRNA sequence of D-2, D-4, D-7, D-15, D-31 and D-33 revealed a high identity of 97 to 99% with 16S rRNA sequence belong to genera Pseudomonas, Marinomonas, Exiquobacterium,Micrococcus, Pseudoalteromonasand Shewanella respectively. Strain D-31 exhibited higher tolerance towards antibiotic with resistance to Kanamycin, Ampicillin and Erythromycin. However, the growth of other strains was retarded by at least two of the antibiotics on their normal growth. The isolation of marine bacterial strain belongs to Marinomonas sp. and Pseudoalteromonassp. create of interest for further biological characterization as the strains from these two genera have been proven for the discovery of new antimicrobial substances, enzymes for industry application and unique secondary metabolites. 2014 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/61038/1/ChongChunShiong2014_IdentificationandCharacterizationofPotentialUsefulBacteria.pdf Thevarajoo, Suganthi and Goh, Kian Mau and Abd. Manan, Fazilah and Ibrahim, Zaharah and Chong, Chun Shiong (2014) Identification and characterization of potential useful bacteria from marine environment. In: BIOJOHOR 2014, 25-27 August, 2015, Johor Bahru, Malaysia.
spellingShingle R Medicine (General)
Thevarajoo, Suganthi
Goh, Kian Mau
Abd. Manan, Fazilah
Ibrahim, Zaharah
Chong, Chun Shiong
Identification and characterization of potential useful bacteria from marine environment
title Identification and characterization of potential useful bacteria from marine environment
title_full Identification and characterization of potential useful bacteria from marine environment
title_fullStr Identification and characterization of potential useful bacteria from marine environment
title_full_unstemmed Identification and characterization of potential useful bacteria from marine environment
title_short Identification and characterization of potential useful bacteria from marine environment
title_sort identification and characterization of potential useful bacteria from marine environment
topic R Medicine (General)
url http://eprints.utm.my/61038/1/ChongChunShiong2014_IdentificationandCharacterizationofPotentialUsefulBacteria.pdf
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