Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic arabidopsis thaliana

Pinoresinol reductase and pinoresinol/lariciresinol reductase play important roles in an early step of lignan biosynthesis in plants. The activities of both enzymes have also been detected in bacteria. In this study, pinZ, which was first isolated as a gene for bacterial pinoresinol reductase, was c...

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Main Authors: Tamura, Masayuki, Tsuji, Yukiko, Kusunose, Tatsuya, Kamimura, Naofumi, Okazawa, Atsushi, Mori, Tetsuya, Nakabayashi, Ryo, Hishiyama, Shojiro, Fukuhara, Yuki, Hara, Hirofumi, Sato-Izawa, Kanna, Muranaka, Toshiya, Saito, Kazuki, Katayama, Yoshihiro, Fukuda, Masao, Masai, Eiji, Kajita, Shinya
Format: Article
Published: Springer 2014
Subjects:
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author Tamura, Masayuki
Tsuji, Yukiko
Kusunose, Tatsuya
Kamimura, Naofumi
Okazawa, Atsushi
Mori, Tetsuya
Nakabayashi, Ryo
Hishiyama, Shojiro
Fukuhara, Yuki
Hara, Hirofumi
Sato-Izawa, Kanna
Muranaka, Toshiya
Saito, Kazuki
Katayama, Yoshihiro
Fukuda, Masao
Masai, Eiji
Kajita, Shinya
author_facet Tamura, Masayuki
Tsuji, Yukiko
Kusunose, Tatsuya
Kamimura, Naofumi
Okazawa, Atsushi
Mori, Tetsuya
Nakabayashi, Ryo
Hishiyama, Shojiro
Fukuhara, Yuki
Hara, Hirofumi
Sato-Izawa, Kanna
Muranaka, Toshiya
Saito, Kazuki
Katayama, Yoshihiro
Fukuda, Masao
Masai, Eiji
Kajita, Shinya
author_sort Tamura, Masayuki
collection ePrints
description Pinoresinol reductase and pinoresinol/lariciresinol reductase play important roles in an early step of lignan biosynthesis in plants. The activities of both enzymes have also been detected in bacteria. In this study, pinZ, which was first isolated as a gene for bacterial pinoresinol reductase, was constitutively expressed in Arabidopsis thaliana under the control of the cauliflower mosaic virus 35S promoter. Higher reductive activity toward pinoresinol was detected in the resultant transgenic plants but not in wild-type plant. Principal component analysis of data from untargeted metabolome analyses of stem, root, and leaf extracts of the wild-type and two independent transgenic lines indicate that pinZ expression caused dynamic metabolic changes in stems, but not in roots and leaves. The metabolome data also suggest that expression of pinZ influenced the metabolisms of lignan and glucosinolates but not so much of neolignans such as guaiacylglycerol-8-O-4'-feruloyl ethers. In-depth quantitative analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) indicated that amounts of pinoresinol and its glucoside form were markedly reduced in the transgenic plant, whereas the amounts of glucoside form of secoisolariciresinol in transgenic roots, leaves, and stems increased. The detected levels of lariciresinol in the transgenic plant following beta-glucosidase treatment also tended to be higher than those in the wild-type plant. Our findings indicate that overexpression of pinZ induces change in lignan compositions and has a major effect not only on lignan biosynthesis but also on biosynthesis of other primary and secondary metabolites.
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spelling utm.eprints-627232017-06-05T02:59:21Z http://eprints.utm.my/62723/ Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic arabidopsis thaliana Tamura, Masayuki Tsuji, Yukiko Kusunose, Tatsuya Kamimura, Naofumi Okazawa, Atsushi Mori, Tetsuya Nakabayashi, Ryo Hishiyama, Shojiro Fukuhara, Yuki Hara, Hirofumi Sato-Izawa, Kanna Muranaka, Toshiya Saito, Kazuki Katayama, Yoshihiro Fukuda, Masao Masai, Eiji Kajita, Shinya T Technology Pinoresinol reductase and pinoresinol/lariciresinol reductase play important roles in an early step of lignan biosynthesis in plants. The activities of both enzymes have also been detected in bacteria. In this study, pinZ, which was first isolated as a gene for bacterial pinoresinol reductase, was constitutively expressed in Arabidopsis thaliana under the control of the cauliflower mosaic virus 35S promoter. Higher reductive activity toward pinoresinol was detected in the resultant transgenic plants but not in wild-type plant. Principal component analysis of data from untargeted metabolome analyses of stem, root, and leaf extracts of the wild-type and two independent transgenic lines indicate that pinZ expression caused dynamic metabolic changes in stems, but not in roots and leaves. The metabolome data also suggest that expression of pinZ influenced the metabolisms of lignan and glucosinolates but not so much of neolignans such as guaiacylglycerol-8-O-4'-feruloyl ethers. In-depth quantitative analysis by liquid chromatography-tandem mass spectrometry (LC-MS/MS) indicated that amounts of pinoresinol and its glucoside form were markedly reduced in the transgenic plant, whereas the amounts of glucoside form of secoisolariciresinol in transgenic roots, leaves, and stems increased. The detected levels of lariciresinol in the transgenic plant following beta-glucosidase treatment also tended to be higher than those in the wild-type plant. Our findings indicate that overexpression of pinZ induces change in lignan compositions and has a major effect not only on lignan biosynthesis but also on biosynthesis of other primary and secondary metabolites. Springer 2014 Article PeerReviewed Tamura, Masayuki and Tsuji, Yukiko and Kusunose, Tatsuya and Kamimura, Naofumi and Okazawa, Atsushi and Mori, Tetsuya and Nakabayashi, Ryo and Hishiyama, Shojiro and Fukuhara, Yuki and Hara, Hirofumi and Sato-Izawa, Kanna and Muranaka, Toshiya and Saito, Kazuki and Katayama, Yoshihiro and Fukuda, Masao and Masai, Eiji and Kajita, Shinya (2014) Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic arabidopsis thaliana. Applied Microbiology and Biotechnology, 98 (19). pp. 8165-8177. ISSN 0175-7598 http://dx.doi.org/10.1007/s00253-014-5934-x DOI:10.1007/s00253-014-5934-x
spellingShingle T Technology
Tamura, Masayuki
Tsuji, Yukiko
Kusunose, Tatsuya
Kamimura, Naofumi
Okazawa, Atsushi
Mori, Tetsuya
Nakabayashi, Ryo
Hishiyama, Shojiro
Fukuhara, Yuki
Hara, Hirofumi
Sato-Izawa, Kanna
Muranaka, Toshiya
Saito, Kazuki
Katayama, Yoshihiro
Fukuda, Masao
Masai, Eiji
Kajita, Shinya
Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic arabidopsis thaliana
title Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic arabidopsis thaliana
title_full Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic arabidopsis thaliana
title_fullStr Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic arabidopsis thaliana
title_full_unstemmed Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic arabidopsis thaliana
title_short Successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic arabidopsis thaliana
title_sort successful expression of a novel bacterial gene for pinoresinol reductase and its effect on lignan biosynthesis in transgenic arabidopsis thaliana
topic T Technology
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