Tyrosinase inhibition, anti-acetylcholinestrase, and antimicrobial activities of the phytochemicals from artocarpus integer var silverstris
The leaves of Gynotroches axillaris were chemically and biologically studied. Sequential extraction of the leaves using petroleum ether, chloroform, and methanol afforded three extracts. Puri fication of pet. ether extract yielded, squalene and β -amyrin palmitate as the m...
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Faculty of Pharmacy
2016
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| author | Salam, Ahmed Abed Mohd. Sirat, Hasnah Taher, Muhammad |
| author_facet | Salam, Ahmed Abed Mohd. Sirat, Hasnah Taher, Muhammad |
| author_sort | Salam, Ahmed Abed |
| collection | ePrints |
| description | The leaves of Gynotroches axillaris were chemically and biologically studied. Sequential extraction of the leaves using petroleum ether, chloroform, and methanol afforded three extracts. Puri fication of pet. ether extract yielded, squalene and β -amyrin palmitate as the major compounds, together with palmitic acid and myristic acid as the minor components. The methanol extract yielded two flavonoids, quercitrin and epicatechin. The isolated compounds were characterized by MS, IR and NMR (1D an d 2D). Anti-acetyl cholinesterase screening using TLC bio-autography assay showed that palmitic acid and myristic acid were the strong est inhibition with detection limit 1.14 and 1.28 μg/ 5 μL respectively. Antibacterial against Gram-positive a nd negative and antifungal activities exhibited that β -amyrin palmitate was the strongest (450-225 μ g/mL) against all the tested microbes. The tyrosinase inhibition assay of extracts and the pure compounds were screened against tyrosinase enzyme. The inhibition percentage ( I %) of methanol extract against tyrosinase enzyme was stronger than the other extracts with value 68.4%. Quercitrin (59%) was found to be the highest in the tyrosinase inhibition activity amongst the pure compounds. To the best of our knowledge, this is first report on the phytochemicals, tyrosinase inhibition, anti-acetycholines terase and antimicrobial activities of the leaves of G. axillaris |
| first_indexed | 2024-03-05T19:59:02Z |
| format | Article |
| id | utm.eprints-68490 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:59:02Z |
| publishDate | 2016 |
| publisher | Faculty of Pharmacy |
| record_format | dspace |
| spelling | utm.eprints-684902017-11-20T08:52:06Z http://eprints.utm.my/68490/ Tyrosinase inhibition, anti-acetylcholinestrase, and antimicrobial activities of the phytochemicals from artocarpus integer var silverstris Salam, Ahmed Abed Mohd. Sirat, Hasnah Taher, Muhammad Q Science The leaves of Gynotroches axillaris were chemically and biologically studied. Sequential extraction of the leaves using petroleum ether, chloroform, and methanol afforded three extracts. Puri fication of pet. ether extract yielded, squalene and β -amyrin palmitate as the major compounds, together with palmitic acid and myristic acid as the minor components. The methanol extract yielded two flavonoids, quercitrin and epicatechin. The isolated compounds were characterized by MS, IR and NMR (1D an d 2D). Anti-acetyl cholinesterase screening using TLC bio-autography assay showed that palmitic acid and myristic acid were the strong est inhibition with detection limit 1.14 and 1.28 μg/ 5 μL respectively. Antibacterial against Gram-positive a nd negative and antifungal activities exhibited that β -amyrin palmitate was the strongest (450-225 μ g/mL) against all the tested microbes. The tyrosinase inhibition assay of extracts and the pure compounds were screened against tyrosinase enzyme. The inhibition percentage ( I %) of methanol extract against tyrosinase enzyme was stronger than the other extracts with value 68.4%. Quercitrin (59%) was found to be the highest in the tyrosinase inhibition activity amongst the pure compounds. To the best of our knowledge, this is first report on the phytochemicals, tyrosinase inhibition, anti-acetycholines terase and antimicrobial activities of the leaves of G. axillaris Faculty of Pharmacy 2016-01-11 Article PeerReviewed Salam, Ahmed Abed and Mohd. Sirat, Hasnah and Taher, Muhammad (2016) Tyrosinase inhibition, anti-acetylcholinestrase, and antimicrobial activities of the phytochemicals from artocarpus integer var silverstris. Pakistan Journal of Pharmaceutical Sciences, 29 (6). pp. 2071-2078. ISSN 1011-601X https://www.researchgate.net/publication/311388414_Tyrosinase_inhibition_anti-acetylcholinesterase_and_antimicrobial_activities_of_the_phytochemicals_from_Gynotroches_axillaris_Blume |
| spellingShingle | Q Science Salam, Ahmed Abed Mohd. Sirat, Hasnah Taher, Muhammad Tyrosinase inhibition, anti-acetylcholinestrase, and antimicrobial activities of the phytochemicals from artocarpus integer var silverstris |
| title | Tyrosinase inhibition, anti-acetylcholinestrase, and antimicrobial activities of the phytochemicals from artocarpus integer var silverstris |
| title_full | Tyrosinase inhibition, anti-acetylcholinestrase, and antimicrobial activities of the phytochemicals from artocarpus integer var silverstris |
| title_fullStr | Tyrosinase inhibition, anti-acetylcholinestrase, and antimicrobial activities of the phytochemicals from artocarpus integer var silverstris |
| title_full_unstemmed | Tyrosinase inhibition, anti-acetylcholinestrase, and antimicrobial activities of the phytochemicals from artocarpus integer var silverstris |
| title_short | Tyrosinase inhibition, anti-acetylcholinestrase, and antimicrobial activities of the phytochemicals from artocarpus integer var silverstris |
| title_sort | tyrosinase inhibition anti acetylcholinestrase and antimicrobial activities of the phytochemicals from artocarpus integer var silverstris |
| topic | Q Science |
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