Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and Poly(Dimethylsiloxane) (PDMS) Polymers
In this paper, a minibioreactor platform made of low cost polymers is presented. The minibioreactor prototype was designed as an alternative solution for carrying out microbial fermentation experiments in laboratory. The minibioreactor prototype has a working volume of 1.5 mL and was fabricated fr...
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| Format: | Article |
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2016
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| _version_ | 1796861623777886208 |
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| author | Halimoon, H. Hussain, A. R. Kouzani, A. Alam, M. N. H. Z. |
| author_facet | Halimoon, H. Hussain, A. R. Kouzani, A. Alam, M. N. H. Z. |
| author_sort | Halimoon, H. |
| collection | ePrints |
| description | In this paper, a minibioreactor platform made of low cost polymers is presented. The minibioreactor prototype was designed as an alternative solution for carrying out microbial fermentation experiments in laboratory. The minibioreactor prototype has a working volume of 1.5 mL and was fabricated from poly(methylmethacrylate) ( PMMA ) and poly(dimethylsiloxane) ( PDMS ) polymers. Cell density was measured online whilst agitation rates and the temperature of the reactor content can be tightly controlled to desired set-point values. As proof-of-concept, various S. cerevisae fermentation experiments were conducted. In every experiment, the minibioreactor operated stably for the entire length of operation which was nearly 40 h with very minimal volume loss i.e. about 2.8 μ L·h -1 at 37 º C. The minibioreactor has the maximum oxygen transfer rate ( OTR ) of 16.6 mmol·L -1 ·h -1 under the agitation rate of 300 rpm. Under these conditions, cell specific growth rate as high as 0.291 h -1 was obtained. The experimental data in the minibioreactor operation was also reproducible using shake flask where similar growth profiles were attained under a similar growth conditions. |
| first_indexed | 2024-03-05T19:59:13Z |
| format | Article |
| id | utm.eprints-68756 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T19:59:13Z |
| publishDate | 2016 |
| record_format | dspace |
| spelling | utm.eprints-687562017-11-20T08:52:18Z http://eprints.utm.my/68756/ Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and Poly(Dimethylsiloxane) (PDMS) Polymers Halimoon, H. Hussain, A. R. Kouzani, A. Alam, M. N. H. Z. TP Chemical technology In this paper, a minibioreactor platform made of low cost polymers is presented. The minibioreactor prototype was designed as an alternative solution for carrying out microbial fermentation experiments in laboratory. The minibioreactor prototype has a working volume of 1.5 mL and was fabricated from poly(methylmethacrylate) ( PMMA ) and poly(dimethylsiloxane) ( PDMS ) polymers. Cell density was measured online whilst agitation rates and the temperature of the reactor content can be tightly controlled to desired set-point values. As proof-of-concept, various S. cerevisae fermentation experiments were conducted. In every experiment, the minibioreactor operated stably for the entire length of operation which was nearly 40 h with very minimal volume loss i.e. about 2.8 μ L·h -1 at 37 º C. The minibioreactor has the maximum oxygen transfer rate ( OTR ) of 16.6 mmol·L -1 ·h -1 under the agitation rate of 300 rpm. Under these conditions, cell specific growth rate as high as 0.291 h -1 was obtained. The experimental data in the minibioreactor operation was also reproducible using shake flask where similar growth profiles were attained under a similar growth conditions. 2016 Article PeerReviewed Halimoon, H. and Hussain, A. R. and Kouzani, A. and Alam, M. N. H. Z. (2016) Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and Poly(Dimethylsiloxane) (PDMS) Polymers. Sains Malaysiana, 45 (6). pp. 969-976. http://www.ukm.my/jsm/pdf_files/SM-PDF-45-6-2016/15%20Hazwan%20Halimoon.pdf |
| spellingShingle | TP Chemical technology Halimoon, H. Hussain, A. R. Kouzani, A. Alam, M. N. H. Z. Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and Poly(Dimethylsiloxane) (PDMS) Polymers |
| title | Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and Poly(Dimethylsiloxane) (PDMS) Polymers |
| title_full | Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and Poly(Dimethylsiloxane) (PDMS) Polymers |
| title_fullStr | Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and Poly(Dimethylsiloxane) (PDMS) Polymers |
| title_full_unstemmed | Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and Poly(Dimethylsiloxane) (PDMS) Polymers |
| title_short | Aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly(methylmethacrylate) (PMMA) and Poly(Dimethylsiloxane) (PDMS) Polymers |
| title_sort | aerobic fermentation of saccharomeyes cerevisae in a miniature bioreactor made of low cost poly methylmethacrylate pmma and poly dimethylsiloxane pdms polymers |
| topic | TP Chemical technology |
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