Chromate detoxification using combination of ChromeBac™ system and immobilized chromate reductase beads
Incomplete removal of chromium from discharge effluent of chromium-based industries is a serious problem due to its deleterious effect. ChromeBac™ is a locally developed Cr(VI) reduction system carried out by aerobic Cr(VI) resistant bacteria followed by chemical precipitation. The locally isolated...
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| Format: | Article |
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Elsevier Ltd
2016
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| author | Ishak, A. F. Abdul Karim, N. Ahmad, W. A. Zakaria, Z. A. |
| author_facet | Ishak, A. F. Abdul Karim, N. Ahmad, W. A. Zakaria, Z. A. |
| author_sort | Ishak, A. F. |
| collection | ePrints |
| description | Incomplete removal of chromium from discharge effluent of chromium-based industries is a serious problem due to its deleterious effect. ChromeBac™ is a locally developed Cr(VI) reduction system carried out by aerobic Cr(VI) resistant bacteria followed by chemical precipitation. The locally isolated Cr(VI) resistant-reducing Acinetobacter haemolyticus EF369508 was immobilized onto carrier materials inside a 50 L bioreactor. Mixture of 10% (v/v) liquid pineapple waste and neutralized Cr(VI) solutions (30–60 mgL−1) was fed into the bioreactor at 0.11 m3 h−1. Around 90% of the initial Cr(VI) was reduced after 24 h of contact inside the bioreactor. Residual Cr(VI) was then further reduced to between 1.0 and 1.5 mgL−1 by immobilized chromate reductase alginate-beads packed in a 10 L flow-through column, after 15 h of contact. Some important characteristics for the chromate reductase activities for A. haemolyticus are as follows; not NADH-dependent, associated with CFE with notable contribution from the membrane fraction, enhanced in the presence of glucose, optimal at pH 7.0, 30 °C, in the presence of 1 mM Co2+ (highest) with Michaelis–Menten constant, Km and maximum reaction rate, Vmax of 184.47 μM and 33.3 nmol/min/mg protein respectively. Ag+ and Hg2+ inhibited the enzyme activity. This study demonstrated the potential of using immobilized chromate reductase beads to further reduce residual Cr(VI) present in the effluent of a ChromeBac™ process, hence reducing the time for overall Cr(VI) treatment process. |
| first_indexed | 2024-03-05T20:03:06Z |
| format | Article |
| id | utm.eprints-72115 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:03:06Z |
| publishDate | 2016 |
| publisher | Elsevier Ltd |
| record_format | dspace |
| spelling | utm.eprints-721152017-11-23T01:37:08Z http://eprints.utm.my/72115/ Chromate detoxification using combination of ChromeBac™ system and immobilized chromate reductase beads Ishak, A. F. Abdul Karim, N. Ahmad, W. A. Zakaria, Z. A. QD Chemistry Incomplete removal of chromium from discharge effluent of chromium-based industries is a serious problem due to its deleterious effect. ChromeBac™ is a locally developed Cr(VI) reduction system carried out by aerobic Cr(VI) resistant bacteria followed by chemical precipitation. The locally isolated Cr(VI) resistant-reducing Acinetobacter haemolyticus EF369508 was immobilized onto carrier materials inside a 50 L bioreactor. Mixture of 10% (v/v) liquid pineapple waste and neutralized Cr(VI) solutions (30–60 mgL−1) was fed into the bioreactor at 0.11 m3 h−1. Around 90% of the initial Cr(VI) was reduced after 24 h of contact inside the bioreactor. Residual Cr(VI) was then further reduced to between 1.0 and 1.5 mgL−1 by immobilized chromate reductase alginate-beads packed in a 10 L flow-through column, after 15 h of contact. Some important characteristics for the chromate reductase activities for A. haemolyticus are as follows; not NADH-dependent, associated with CFE with notable contribution from the membrane fraction, enhanced in the presence of glucose, optimal at pH 7.0, 30 °C, in the presence of 1 mM Co2+ (highest) with Michaelis–Menten constant, Km and maximum reaction rate, Vmax of 184.47 μM and 33.3 nmol/min/mg protein respectively. Ag+ and Hg2+ inhibited the enzyme activity. This study demonstrated the potential of using immobilized chromate reductase beads to further reduce residual Cr(VI) present in the effluent of a ChromeBac™ process, hence reducing the time for overall Cr(VI) treatment process. Elsevier Ltd 2016 Article PeerReviewed Ishak, A. F. and Abdul Karim, N. and Ahmad, W. A. and Zakaria, Z. A. (2016) Chromate detoxification using combination of ChromeBac™ system and immobilized chromate reductase beads. International Biodeterioration and Biodegradation, 113 . pp. 238-243. ISSN 0964-8305 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84962304541&doi=10.1016%2fj.ibiod.2016.03.020&partnerID=40&md5=92090b15f985322a3c151a1916ba6f06 |
| spellingShingle | QD Chemistry Ishak, A. F. Abdul Karim, N. Ahmad, W. A. Zakaria, Z. A. Chromate detoxification using combination of ChromeBac™ system and immobilized chromate reductase beads |
| title | Chromate detoxification using combination of ChromeBac™ system and immobilized chromate reductase beads |
| title_full | Chromate detoxification using combination of ChromeBac™ system and immobilized chromate reductase beads |
| title_fullStr | Chromate detoxification using combination of ChromeBac™ system and immobilized chromate reductase beads |
| title_full_unstemmed | Chromate detoxification using combination of ChromeBac™ system and immobilized chromate reductase beads |
| title_short | Chromate detoxification using combination of ChromeBac™ system and immobilized chromate reductase beads |
| title_sort | chromate detoxification using combination of chromebac™ system and immobilized chromate reductase beads |
| topic | QD Chemistry |
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