Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology
The objective of this study was to develop an optimized assay for Salmonella Typhi biofilm that mimics the environment of the gallbladder as an experimental model for chronic typhoid fever. Multi-factorial assays are difficult to optimize using traditional one-factor-at-a-time optimization methods....
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| Format: | Article |
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Taylor and Francis Ltd.
2016
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| author | Ganjali Dashti, M. Abdeshahian, P. Sudesh, K. Phua, K. K. |
| author_facet | Ganjali Dashti, M. Abdeshahian, P. Sudesh, K. Phua, K. K. |
| author_sort | Ganjali Dashti, M. |
| collection | ePrints |
| description | The objective of this study was to develop an optimized assay for Salmonella Typhi biofilm that mimics the environment of the gallbladder as an experimental model for chronic typhoid fever. Multi-factorial assays are difficult to optimize using traditional one-factor-at-a-time optimization methods. Response surface methodology (RSM) was used to optimize six key variables involved in S. Typhi biofilm formation on cholesterol-coated polypropylene 96-well microtiter plates. The results showed that bile (1.22%), glucose (2%), cholesterol (0.05%) and potassium chloride (0.25%) were critical factors affecting the amount of biofilm produced, but agitation (275 rpm) and sodium chloride (0.5%) had antagonistic effects on each other. Under these optimum conditions the maximum OD reading for biofilm formation was 3.4 (λ600 nm), and the coefficients of variation for intra-plate and inter-plate assays were 3% (n = 20) and 5% (n = 8), respectively. These results showed that RSM is an effective approach for biofilm assay optimization. |
| first_indexed | 2024-03-05T20:04:20Z |
| format | Article |
| id | utm.eprints-72623 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:04:20Z |
| publishDate | 2016 |
| publisher | Taylor and Francis Ltd. |
| record_format | dspace |
| spelling | utm.eprints-726232017-11-27T04:37:36Z http://eprints.utm.my/72623/ Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology Ganjali Dashti, M. Abdeshahian, P. Sudesh, K. Phua, K. K. TP Chemical technology The objective of this study was to develop an optimized assay for Salmonella Typhi biofilm that mimics the environment of the gallbladder as an experimental model for chronic typhoid fever. Multi-factorial assays are difficult to optimize using traditional one-factor-at-a-time optimization methods. Response surface methodology (RSM) was used to optimize six key variables involved in S. Typhi biofilm formation on cholesterol-coated polypropylene 96-well microtiter plates. The results showed that bile (1.22%), glucose (2%), cholesterol (0.05%) and potassium chloride (0.25%) were critical factors affecting the amount of biofilm produced, but agitation (275 rpm) and sodium chloride (0.5%) had antagonistic effects on each other. Under these optimum conditions the maximum OD reading for biofilm formation was 3.4 (λ600 nm), and the coefficients of variation for intra-plate and inter-plate assays were 3% (n = 20) and 5% (n = 8), respectively. These results showed that RSM is an effective approach for biofilm assay optimization. Taylor and Francis Ltd. 2016 Article PeerReviewed Ganjali Dashti, M. and Abdeshahian, P. and Sudesh, K. and Phua, K. K. (2016) Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology. Biofouling, 32 (4). pp. 477-487. ISSN 0892-7014 https://www.scopus.com/inward/record.uri?eid=2-s2.0-84961891807&doi=10.1080%2f08927014.2015.1135328&partnerID=40&md5=05d25c3a2f5bb0e884a53d3d0eae05fc |
| spellingShingle | TP Chemical technology Ganjali Dashti, M. Abdeshahian, P. Sudesh, K. Phua, K. K. Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology |
| title | Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology |
| title_full | Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology |
| title_fullStr | Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology |
| title_full_unstemmed | Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology |
| title_short | Optimization of Salmonella Typhi biofilm assay on polypropylene microtiter plates using response surface methodology |
| title_sort | optimization of salmonella typhi biofilm assay on polypropylene microtiter plates using response surface methodology |
| topic | TP Chemical technology |
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