A factorial analysis study on enzymatic hydrolysis of fiber pressed oil palm frond for bioethanol production
Different technologies have been developed to for the conversion of lignocellulosic biomass to suitable fermentation substrates for bioethanol production. The enzymatic conversion of cellulose seems to be the most promising technology as it is highly specific and does not produce substantial amounts...
| Main Authors: | , , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Published: |
Institute of Physics Publishing
2016
|
| Subjects: |
| _version_ | 1796862055489208320 |
|---|---|
| author | Hashim, F. S. Yussof, H. W. Zahari, M. A. K. M. Illias, R. M. Rahman, R. A. |
| author_facet | Hashim, F. S. Yussof, H. W. Zahari, M. A. K. M. Illias, R. M. Rahman, R. A. |
| author_sort | Hashim, F. S. |
| collection | ePrints |
| description | Different technologies have been developed to for the conversion of lignocellulosic biomass to suitable fermentation substrates for bioethanol production. The enzymatic conversion of cellulose seems to be the most promising technology as it is highly specific and does not produce substantial amounts of unwanted byproducts. The effects of agitation speed, enzyme loading, temperature, pH and reaction time on the conversion of glucose from fiber pressed oil palm frond (FPOPF) for bioethanol production were screened by statistical analysis using response surface methodology (RSM). A half fraction two-level factorial analysis with five factors was selected for the experimental design to determine the best enzymatic conditions that produce maximum amount of glucose. FPOPF was pre-treated with alkaline prior to enzymatic hydrolysis. The enzymatic hydrolysis was performed using a commercial enzyme Cellic CTec2. From this study, the highest yield of glucose concentration was 9.736 g/L at 72 hours reaction time at 35 °C, pH 5.6, and 1.5% (w/v) of enzyme loading. The model obtained was significant with p-value <0.0001. It is suggested that this model had a maximum point which is likely to be the optimum point and possible for the optimization process. |
| first_indexed | 2024-03-05T20:05:46Z |
| format | Conference or Workshop Item |
| id | utm.eprints-73284 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:05:46Z |
| publishDate | 2016 |
| publisher | Institute of Physics Publishing |
| record_format | dspace |
| spelling | utm.eprints-732842017-11-27T02:00:02Z http://eprints.utm.my/73284/ A factorial analysis study on enzymatic hydrolysis of fiber pressed oil palm frond for bioethanol production Hashim, F. S. Yussof, H. W. Zahari, M. A. K. M. Illias, R. M. Rahman, R. A. TP Chemical technology Different technologies have been developed to for the conversion of lignocellulosic biomass to suitable fermentation substrates for bioethanol production. The enzymatic conversion of cellulose seems to be the most promising technology as it is highly specific and does not produce substantial amounts of unwanted byproducts. The effects of agitation speed, enzyme loading, temperature, pH and reaction time on the conversion of glucose from fiber pressed oil palm frond (FPOPF) for bioethanol production were screened by statistical analysis using response surface methodology (RSM). A half fraction two-level factorial analysis with five factors was selected for the experimental design to determine the best enzymatic conditions that produce maximum amount of glucose. FPOPF was pre-treated with alkaline prior to enzymatic hydrolysis. The enzymatic hydrolysis was performed using a commercial enzyme Cellic CTec2. From this study, the highest yield of glucose concentration was 9.736 g/L at 72 hours reaction time at 35 °C, pH 5.6, and 1.5% (w/v) of enzyme loading. The model obtained was significant with p-value <0.0001. It is suggested that this model had a maximum point which is likely to be the optimum point and possible for the optimization process. Institute of Physics Publishing 2016 Conference or Workshop Item PeerReviewed Hashim, F. S. and Yussof, H. W. and Zahari, M. A. K. M. and Illias, R. M. and Rahman, R. A. (2016) A factorial analysis study on enzymatic hydrolysis of fiber pressed oil palm frond for bioethanol production. In: 2nd International Conference on Advances in Renewable Energy and Technologies, ICARET 2016, 23 February 2016 through 25 February 2016, Putrajaya; Malaysia. https://www.scopus.com/inward/record.uri?eid=2-s2.0-84966641674&doi=10.1088%2f1755-1315%2f32%2f1%2f012071&partnerID=40&md5=010b53db9fcc0afc89bf6f16680b08c1 |
| spellingShingle | TP Chemical technology Hashim, F. S. Yussof, H. W. Zahari, M. A. K. M. Illias, R. M. Rahman, R. A. A factorial analysis study on enzymatic hydrolysis of fiber pressed oil palm frond for bioethanol production |
| title | A factorial analysis study on enzymatic hydrolysis of fiber pressed oil palm frond for bioethanol production |
| title_full | A factorial analysis study on enzymatic hydrolysis of fiber pressed oil palm frond for bioethanol production |
| title_fullStr | A factorial analysis study on enzymatic hydrolysis of fiber pressed oil palm frond for bioethanol production |
| title_full_unstemmed | A factorial analysis study on enzymatic hydrolysis of fiber pressed oil palm frond for bioethanol production |
| title_short | A factorial analysis study on enzymatic hydrolysis of fiber pressed oil palm frond for bioethanol production |
| title_sort | factorial analysis study on enzymatic hydrolysis of fiber pressed oil palm frond for bioethanol production |
| topic | TP Chemical technology |
| work_keys_str_mv | AT hashimfs afactorialanalysisstudyonenzymatichydrolysisoffiberpressedoilpalmfrondforbioethanolproduction AT yussofhw afactorialanalysisstudyonenzymatichydrolysisoffiberpressedoilpalmfrondforbioethanolproduction AT zaharimakm afactorialanalysisstudyonenzymatichydrolysisoffiberpressedoilpalmfrondforbioethanolproduction AT illiasrm afactorialanalysisstudyonenzymatichydrolysisoffiberpressedoilpalmfrondforbioethanolproduction AT rahmanra afactorialanalysisstudyonenzymatichydrolysisoffiberpressedoilpalmfrondforbioethanolproduction AT hashimfs factorialanalysisstudyonenzymatichydrolysisoffiberpressedoilpalmfrondforbioethanolproduction AT yussofhw factorialanalysisstudyonenzymatichydrolysisoffiberpressedoilpalmfrondforbioethanolproduction AT zaharimakm factorialanalysisstudyonenzymatichydrolysisoffiberpressedoilpalmfrondforbioethanolproduction AT illiasrm factorialanalysisstudyonenzymatichydrolysisoffiberpressedoilpalmfrondforbioethanolproduction AT rahmanra factorialanalysisstudyonenzymatichydrolysisoffiberpressedoilpalmfrondforbioethanolproduction |