Bioprocess development for pleuran production by pleurotus ostreatus using submerged cultivation system in semi-industrial scale
Nowadays, the demand of new natural and safe pharmaceutical production of immunomodulatory and anticancer properties is very high. For many years it have been reported that Pleurotus ostreatus is characterized by pleuran production, a b-1(1,3/1,6)-D-glucan biopolymer. This polysaccharide has binding...
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| Format: | Conference or Workshop Item |
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AIChE
2016
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| _version_ | 1796862096205414400 |
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| author | El Enshasy, H. A. Johari Masri, M. H. Malek, R. A. Othman, N. Z. Leng, O. M. Aziz, R. |
| author_facet | El Enshasy, H. A. Johari Masri, M. H. Malek, R. A. Othman, N. Z. Leng, O. M. Aziz, R. |
| author_sort | El Enshasy, H. A. |
| collection | ePrints |
| description | Nowadays, the demand of new natural and safe pharmaceutical production of immunomodulatory and anticancer properties is very high. For many years it have been reported that Pleurotus ostreatus is characterized by pleuran production, a b-1(1,3/1,6)-D-glucan biopolymer. This polysaccharide has binding affinity to carbohydrate receptors on immune cells and thus have good immunomodulatory activity. When cells cultivated in submerged culture system, they are able to production the pleuran as exopolysaccharide (EPS) in the fermentation medium. In this work, a complete industrial platform was developed to increase the pleuran production using submerged cultivation system in semi-industrial scale. At first, different media formulations were tested for polysaccharide production. The medium of highest EPS production was optimized using statistical approach in shake flask level. After this step, cultivations were conducted in 16-L stirred tank bioreactor for bioprocess industrialization and optimization of different bioprocessing engineering parameters to develop complete industrial platform for this polysaccharide in Semi-industrial scale. |
| first_indexed | 2024-03-05T20:06:23Z |
| format | Conference or Workshop Item |
| id | utm.eprints-73574 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:06:23Z |
| publishDate | 2016 |
| publisher | AIChE |
| record_format | dspace |
| spelling | utm.eprints-735742017-11-26T08:07:48Z http://eprints.utm.my/73574/ Bioprocess development for pleuran production by pleurotus ostreatus using submerged cultivation system in semi-industrial scale El Enshasy, H. A. Johari Masri, M. H. Malek, R. A. Othman, N. Z. Leng, O. M. Aziz, R. TP Chemical technology Nowadays, the demand of new natural and safe pharmaceutical production of immunomodulatory and anticancer properties is very high. For many years it have been reported that Pleurotus ostreatus is characterized by pleuran production, a b-1(1,3/1,6)-D-glucan biopolymer. This polysaccharide has binding affinity to carbohydrate receptors on immune cells and thus have good immunomodulatory activity. When cells cultivated in submerged culture system, they are able to production the pleuran as exopolysaccharide (EPS) in the fermentation medium. In this work, a complete industrial platform was developed to increase the pleuran production using submerged cultivation system in semi-industrial scale. At first, different media formulations were tested for polysaccharide production. The medium of highest EPS production was optimized using statistical approach in shake flask level. After this step, cultivations were conducted in 16-L stirred tank bioreactor for bioprocess industrialization and optimization of different bioprocessing engineering parameters to develop complete industrial platform for this polysaccharide in Semi-industrial scale. AIChE 2016 Conference or Workshop Item PeerReviewed El Enshasy, H. A. and Johari Masri, M. H. and Malek, R. A. and Othman, N. Z. and Leng, O. M. and Aziz, R. (2016) Bioprocess development for pleuran production by pleurotus ostreatus using submerged cultivation system in semi-industrial scale. In: Food, Pharmaceutical and Bioengineering Division 2016 - Core Programming Area at the 2016 AIChE Annual Meeting, 13-18 Nov 2016, San Francisco, United States. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019097399&partnerID=40&md5=86dbe741e0dddaba0ea6ff35a3e4b797 |
| spellingShingle | TP Chemical technology El Enshasy, H. A. Johari Masri, M. H. Malek, R. A. Othman, N. Z. Leng, O. M. Aziz, R. Bioprocess development for pleuran production by pleurotus ostreatus using submerged cultivation system in semi-industrial scale |
| title | Bioprocess development for pleuran production by pleurotus ostreatus using submerged cultivation system in semi-industrial scale |
| title_full | Bioprocess development for pleuran production by pleurotus ostreatus using submerged cultivation system in semi-industrial scale |
| title_fullStr | Bioprocess development for pleuran production by pleurotus ostreatus using submerged cultivation system in semi-industrial scale |
| title_full_unstemmed | Bioprocess development for pleuran production by pleurotus ostreatus using submerged cultivation system in semi-industrial scale |
| title_short | Bioprocess development for pleuran production by pleurotus ostreatus using submerged cultivation system in semi-industrial scale |
| title_sort | bioprocess development for pleuran production by pleurotus ostreatus using submerged cultivation system in semi industrial scale |
| topic | TP Chemical technology |
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