Influence of seed pre-soaking and oil body washing regime on the physical quality of the final suspension

Obtaining small intact organelles is crucial to avoid artefacts in oil body suspension or emulsion. This work was undertaken to recover high quality of oil bodies from sunflower seeds. Organelle integrity could be lost if high forces are imposed on the tissue/material during their recovery. Pre-soak...

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Main Authors: Taher, Z. M., Foster, T., Gray, D.
Format: Conference or Workshop Item
Published: AIChE 2016
Subjects:
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author Taher, Z. M.
Foster, T.
Gray, D.
author_facet Taher, Z. M.
Foster, T.
Gray, D.
author_sort Taher, Z. M.
collection ePrints
description Obtaining small intact organelles is crucial to avoid artefacts in oil body suspension or emulsion. This work was undertaken to recover high quality of oil bodies from sunflower seeds. Organelle integrity could be lost if high forces are imposed on the tissue/material during their recovery. Pre-soaking before wet milling and multiple washing with alkaline buffer was performed to isolate a high quality oil bodies suspension. It was evident from different physical measurements like particle size, zeta-potential and light microscopy that pre-soaking had a significant influence on the oil body suspensions with no significant sign of aggregation or coalescence. It was also observed that the resultant washed oil body suspensions are highly surface charge indicating very stable suspension phase behaviour. The physical state of the seed during wet milling has a significant impact on the quality of the oil body suspension.
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spelling utm.eprints-736482017-11-28T06:50:01Z http://eprints.utm.my/73648/ Influence of seed pre-soaking and oil body washing regime on the physical quality of the final suspension Taher, Z. M. Foster, T. Gray, D. TP Chemical technology Obtaining small intact organelles is crucial to avoid artefacts in oil body suspension or emulsion. This work was undertaken to recover high quality of oil bodies from sunflower seeds. Organelle integrity could be lost if high forces are imposed on the tissue/material during their recovery. Pre-soaking before wet milling and multiple washing with alkaline buffer was performed to isolate a high quality oil bodies suspension. It was evident from different physical measurements like particle size, zeta-potential and light microscopy that pre-soaking had a significant influence on the oil body suspensions with no significant sign of aggregation or coalescence. It was also observed that the resultant washed oil body suspensions are highly surface charge indicating very stable suspension phase behaviour. The physical state of the seed during wet milling has a significant impact on the quality of the oil body suspension. AIChE 2016 Conference or Workshop Item PeerReviewed Taher, Z. M. and Foster, T. and Gray, D. (2016) Influence of seed pre-soaking and oil body washing regime on the physical quality of the final suspension. In: Food, Pharmaceutical and Bioengineering Division 2016 - Core Programming Area at the 2016 AIChE Annual Meeting, 13-18 Nov 2016, San Francisco, United States. https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019137797&partnerID=40&md5=57d0d97e4a05f25592bb1d59510d1b13
spellingShingle TP Chemical technology
Taher, Z. M.
Foster, T.
Gray, D.
Influence of seed pre-soaking and oil body washing regime on the physical quality of the final suspension
title Influence of seed pre-soaking and oil body washing regime on the physical quality of the final suspension
title_full Influence of seed pre-soaking and oil body washing regime on the physical quality of the final suspension
title_fullStr Influence of seed pre-soaking and oil body washing regime on the physical quality of the final suspension
title_full_unstemmed Influence of seed pre-soaking and oil body washing regime on the physical quality of the final suspension
title_short Influence of seed pre-soaking and oil body washing regime on the physical quality of the final suspension
title_sort influence of seed pre soaking and oil body washing regime on the physical quality of the final suspension
topic TP Chemical technology
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AT fostert influenceofseedpresoakingandoilbodywashingregimeonthephysicalqualityofthefinalsuspension
AT grayd influenceofseedpresoakingandoilbodywashingregimeonthephysicalqualityofthefinalsuspension