Reaction optimization of Aspergillus niger α-L-arabinofuranosidase for improved arabinose production from kenaf stem
There are abundant of lignocellulosic biomass readily available with varying compositions. Kenaf (Hibiscus cannabinus) is one of this lignocellulosic biomass that has a high content of hemicellulose. This particular hemicellulose is composed of high arabinoxylan, which is a xylan backbone with arabi...
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| Format: | Article |
| Language: | English |
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Penerbit UTM Press
2017
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| Online Access: | http://eprints.utm.my/76708/1/FarahDibaAbu2017_ReactionOptimizationofAspergillusNiger.pdf |
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| author | Ayob, S. N. Abdul Murad, A. M. Abu Bakar, F. D. Md. Illias, R. |
| author_facet | Ayob, S. N. Abdul Murad, A. M. Abu Bakar, F. D. Md. Illias, R. |
| author_sort | Ayob, S. N. |
| collection | ePrints |
| description | There are abundant of lignocellulosic biomass readily available with varying compositions. Kenaf (Hibiscus cannabinus) is one of this lignocellulosic biomass that has a high content of hemicellulose. This particular hemicellulose is composed of high arabinoxylan, which is a xylan backbone with arabinofuranosyl branches. In order to hydrolyze arabinoxylan, a branching enzyme is needed. Therefore, α-L-arabinofuranosidase from Aspergillus niger ATCC120120 (AnabfA) was used to hydrolyzed pre-treated kenaf and the reaction conditions were optimized using central composite design (CCD) to produce a significant amount of arabinose. There were 20 experiments conducted with 1.68 star points and 6 replicates at the centre points. The reaction conditions that were optimized are enzyme loading, substrate concentration and reaction time in which resulted with 88 U AnabfA activity, 0.9% (w/v) and 48 h, respectively. These optimized conditions managed to increase the yield of arabinose with 47.17 mg/g arabinose produced. |
| first_indexed | 2024-03-05T20:13:26Z |
| format | Article |
| id | utm.eprints-76708 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T20:13:26Z |
| publishDate | 2017 |
| publisher | Penerbit UTM Press |
| record_format | dspace |
| spelling | utm.eprints-767082018-05-31T09:25:31Z http://eprints.utm.my/76708/ Reaction optimization of Aspergillus niger α-L-arabinofuranosidase for improved arabinose production from kenaf stem Ayob, S. N. Abdul Murad, A. M. Abu Bakar, F. D. Md. Illias, R. TP Chemical technology There are abundant of lignocellulosic biomass readily available with varying compositions. Kenaf (Hibiscus cannabinus) is one of this lignocellulosic biomass that has a high content of hemicellulose. This particular hemicellulose is composed of high arabinoxylan, which is a xylan backbone with arabinofuranosyl branches. In order to hydrolyze arabinoxylan, a branching enzyme is needed. Therefore, α-L-arabinofuranosidase from Aspergillus niger ATCC120120 (AnabfA) was used to hydrolyzed pre-treated kenaf and the reaction conditions were optimized using central composite design (CCD) to produce a significant amount of arabinose. There were 20 experiments conducted with 1.68 star points and 6 replicates at the centre points. The reaction conditions that were optimized are enzyme loading, substrate concentration and reaction time in which resulted with 88 U AnabfA activity, 0.9% (w/v) and 48 h, respectively. These optimized conditions managed to increase the yield of arabinose with 47.17 mg/g arabinose produced. Penerbit UTM Press 2017 Article PeerReviewed application/pdf en http://eprints.utm.my/76708/1/FarahDibaAbu2017_ReactionOptimizationofAspergillusNiger.pdf Ayob, S. N. and Abdul Murad, A. M. and Abu Bakar, F. D. and Md. Illias, R. (2017) Reaction optimization of Aspergillus niger α-L-arabinofuranosidase for improved arabinose production from kenaf stem. Jurnal Teknologi, 79 (4). pp. 137-145. ISSN 0127-9696 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85018360068&doi=10.11113%2fjt.v79.10558&partnerID=40&md5=910a8d91ea7eb00b391d54d4b1f898b9 DOI:10.11113/jt.v79.10558 |
| spellingShingle | TP Chemical technology Ayob, S. N. Abdul Murad, A. M. Abu Bakar, F. D. Md. Illias, R. Reaction optimization of Aspergillus niger α-L-arabinofuranosidase for improved arabinose production from kenaf stem |
| title | Reaction optimization of Aspergillus niger α-L-arabinofuranosidase for improved arabinose production from kenaf stem |
| title_full | Reaction optimization of Aspergillus niger α-L-arabinofuranosidase for improved arabinose production from kenaf stem |
| title_fullStr | Reaction optimization of Aspergillus niger α-L-arabinofuranosidase for improved arabinose production from kenaf stem |
| title_full_unstemmed | Reaction optimization of Aspergillus niger α-L-arabinofuranosidase for improved arabinose production from kenaf stem |
| title_short | Reaction optimization of Aspergillus niger α-L-arabinofuranosidase for improved arabinose production from kenaf stem |
| title_sort | reaction optimization of aspergillus niger α l arabinofuranosidase for improved arabinose production from kenaf stem |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/76708/1/FarahDibaAbu2017_ReactionOptimizationofAspergillusNiger.pdf |
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