Isolation of potential bacteria as inoculum for biofloc formation in pacific whiteleg shrimp, litopenaeus vannamei culture ponds
Background and Objective: A new green technology to reduce environmental damages while optimizing production of Pacific Whiteleg shrimp, Litopenaeus vannamei was developed known as “Biofloc technology”. Microbial communities in biofloc aggregates are responsible in eliminating water exchange and pro...
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| Format: | Article |
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Asian Network for Scientific Information
2017
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| author | Kasan, N. A. Ghazali, N. A. Ikhwanuddin, M. Ibrahim, Z. |
| author_facet | Kasan, N. A. Ghazali, N. A. Ikhwanuddin, M. Ibrahim, Z. |
| author_sort | Kasan, N. A. |
| collection | ePrints |
| description | Background and Objective: A new green technology to reduce environmental damages while optimizing production of Pacific Whiteleg shrimp, Litopenaeus vannamei was developed known as “Biofloc technology”. Microbial communities in biofloc aggregates are responsible in eliminating water exchange and producing microbial proteins that can be used as supplemented feed for L. vannamei. This study aimed to isolate and identify potential bioflocculant-producing bacteria to be used as inoculum for rapid formation of biofloc. Materials and Methods: For the purpose of this study, bacterial communities during 0, 30 and 70 days of culture (DOC) of L. vannamei grow-out ponds were isolated and identified through phenotypic and 16S rDNA sequences analysis. Phylogenetic relationships between isolated bacteria were then evaluated through phylogenetic tree analysis. One-way analysis of variance (ANOVA) was used to compare the differences of microbial communities at each DOC. Results: Out of 125 bacterial isolates, nine species of bacteria from biofloc were identified successfully. Those bacteria species were identified as Halomonas venusta, H. aquamarina, Vibrio parahaemolyticus, Bacillus infantis, B. cereus, B. safensis, Providencia vermicola, Nitratireductor aquimarinus and Pseudoalteromonas sp., respectively. Through phylogenetic analysis, these isolates belong to Proteobacteria and Firmicutes families under the genera of Halomonas sp., Vibrio sp., Bacillus sp., Providencia sp., Nitratireductor sp. and Pseudoalteromonas sp. Conclusion: In this study, bioflocculant-producing bacteria were successfully identified which are perfect candidates in forming biofloc to reduce water pollution towards a sustainable aquaculture industry. Presence of Halomonas sp. and Bacillus sp. in all stages of biofloc formation reinforces the need for new development regarding the ability of these species to be used as inoculum in forming biofloc rapidly. |
| first_indexed | 2024-03-05T20:14:15Z |
| format | Article |
| id | utm.eprints-77005 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:14:15Z |
| publishDate | 2017 |
| publisher | Asian Network for Scientific Information |
| record_format | dspace |
| spelling | utm.eprints-770052018-04-30T14:32:33Z http://eprints.utm.my/77005/ Isolation of potential bacteria as inoculum for biofloc formation in pacific whiteleg shrimp, litopenaeus vannamei culture ponds Kasan, N. A. Ghazali, N. A. Ikhwanuddin, M. Ibrahim, Z. QH301 Biology Background and Objective: A new green technology to reduce environmental damages while optimizing production of Pacific Whiteleg shrimp, Litopenaeus vannamei was developed known as “Biofloc technology”. Microbial communities in biofloc aggregates are responsible in eliminating water exchange and producing microbial proteins that can be used as supplemented feed for L. vannamei. This study aimed to isolate and identify potential bioflocculant-producing bacteria to be used as inoculum for rapid formation of biofloc. Materials and Methods: For the purpose of this study, bacterial communities during 0, 30 and 70 days of culture (DOC) of L. vannamei grow-out ponds were isolated and identified through phenotypic and 16S rDNA sequences analysis. Phylogenetic relationships between isolated bacteria were then evaluated through phylogenetic tree analysis. One-way analysis of variance (ANOVA) was used to compare the differences of microbial communities at each DOC. Results: Out of 125 bacterial isolates, nine species of bacteria from biofloc were identified successfully. Those bacteria species were identified as Halomonas venusta, H. aquamarina, Vibrio parahaemolyticus, Bacillus infantis, B. cereus, B. safensis, Providencia vermicola, Nitratireductor aquimarinus and Pseudoalteromonas sp., respectively. Through phylogenetic analysis, these isolates belong to Proteobacteria and Firmicutes families under the genera of Halomonas sp., Vibrio sp., Bacillus sp., Providencia sp., Nitratireductor sp. and Pseudoalteromonas sp. Conclusion: In this study, bioflocculant-producing bacteria were successfully identified which are perfect candidates in forming biofloc to reduce water pollution towards a sustainable aquaculture industry. Presence of Halomonas sp. and Bacillus sp. in all stages of biofloc formation reinforces the need for new development regarding the ability of these species to be used as inoculum in forming biofloc rapidly. Asian Network for Scientific Information 2017 Article PeerReviewed Kasan, N. A. and Ghazali, N. A. and Ikhwanuddin, M. and Ibrahim, Z. (2017) Isolation of potential bacteria as inoculum for biofloc formation in pacific whiteleg shrimp, litopenaeus vannamei culture ponds. Pakistan Journal of Biological Sciences, 20 (6). pp. 306-313. ISSN 1028-8880 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85026919115&doi=10.3923%2fpjbs.2017.306.313&partnerID=40&md5=e550ab42da5970ecc35c73d5ab7e28c3 DOI:10.3923/pjbs.2017.306.313 |
| spellingShingle | QH301 Biology Kasan, N. A. Ghazali, N. A. Ikhwanuddin, M. Ibrahim, Z. Isolation of potential bacteria as inoculum for biofloc formation in pacific whiteleg shrimp, litopenaeus vannamei culture ponds |
| title | Isolation of potential bacteria as inoculum for biofloc formation in pacific whiteleg shrimp, litopenaeus vannamei culture ponds |
| title_full | Isolation of potential bacteria as inoculum for biofloc formation in pacific whiteleg shrimp, litopenaeus vannamei culture ponds |
| title_fullStr | Isolation of potential bacteria as inoculum for biofloc formation in pacific whiteleg shrimp, litopenaeus vannamei culture ponds |
| title_full_unstemmed | Isolation of potential bacteria as inoculum for biofloc formation in pacific whiteleg shrimp, litopenaeus vannamei culture ponds |
| title_short | Isolation of potential bacteria as inoculum for biofloc formation in pacific whiteleg shrimp, litopenaeus vannamei culture ponds |
| title_sort | isolation of potential bacteria as inoculum for biofloc formation in pacific whiteleg shrimp litopenaeus vannamei culture ponds |
| topic | QH301 Biology |
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