Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system
Background: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4 billion by 2020. Results: The production medium was first optimized using a statistical optimization approach...
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| Format: | Article |
| Language: | English |
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BioMed Central Ltd.
2018
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| Online Access: | http://eprints.utm.my/79657/1/RoslindaAbdMalek2018_BioprocessOptimizationforPectinaseProduction.pdf |
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| author | El Enshasy, H. A. Elsayed, E. A. Suhaimi, N. Malek, R. A. Esawy, M. |
| author_facet | El Enshasy, H. A. Elsayed, E. A. Suhaimi, N. Malek, R. A. Esawy, M. |
| author_sort | El Enshasy, H. A. |
| collection | ePrints |
| description | Background: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4 billion by 2020. Results: The production medium was first optimized using a statistical optimization approach to increase pectinase production. A maximal enzyme concentration of 76.35 U/mL (a 2.8-fold increase compared with the initial medium) was produced in a medium composed of (g/L): pectin, 32.22; (NH4)2SO4, 4.33; K2HPO4, 1.36; MgSO4.5H2O, 0.05; KCl, 0.05; and FeSO4.5H2O, 0.10. The cultivations were then carried out in a 16-L stirred tank bioreactor in both batch and fed-batch modes to improve enzyme production, which is an important step for bioprocess industrialization. Controlling the pH at 5.5 during cultivation yielded a pectinase production of 109.63 U/mL, which was about 10% higher than the uncontrolled pH culture. Furthermore, fed-batch cultivation using sucrose as a feeding substrate with a rate of 2 g/L/h increased the enzyme production up to 450 U/mL after 126 h. Conclusions: Statistical medium optimization improved volumetric pectinase productivity by about 2.8 folds. Scaling-up the production process in 16-L semi-industrial stirred tank bioreactor under controlled pH further enhanced pectinase production by about 4-folds. Finally, bioreactor fed-batch cultivation using constant carbon source feeding increased maximal volumetric enzyme production by about 16.5-folds from the initial starting conditions. |
| first_indexed | 2024-03-05T20:21:23Z |
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| id | utm.eprints-79657 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T20:21:23Z |
| publishDate | 2018 |
| publisher | BioMed Central Ltd. |
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| spelling | utm.eprints-796572019-01-28T04:58:28Z http://eprints.utm.my/79657/ Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system El Enshasy, H. A. Elsayed, E. A. Suhaimi, N. Malek, R. A. Esawy, M. TP Chemical technology Background: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4 billion by 2020. Results: The production medium was first optimized using a statistical optimization approach to increase pectinase production. A maximal enzyme concentration of 76.35 U/mL (a 2.8-fold increase compared with the initial medium) was produced in a medium composed of (g/L): pectin, 32.22; (NH4)2SO4, 4.33; K2HPO4, 1.36; MgSO4.5H2O, 0.05; KCl, 0.05; and FeSO4.5H2O, 0.10. The cultivations were then carried out in a 16-L stirred tank bioreactor in both batch and fed-batch modes to improve enzyme production, which is an important step for bioprocess industrialization. Controlling the pH at 5.5 during cultivation yielded a pectinase production of 109.63 U/mL, which was about 10% higher than the uncontrolled pH culture. Furthermore, fed-batch cultivation using sucrose as a feeding substrate with a rate of 2 g/L/h increased the enzyme production up to 450 U/mL after 126 h. Conclusions: Statistical medium optimization improved volumetric pectinase productivity by about 2.8 folds. Scaling-up the production process in 16-L semi-industrial stirred tank bioreactor under controlled pH further enhanced pectinase production by about 4-folds. Finally, bioreactor fed-batch cultivation using constant carbon source feeding increased maximal volumetric enzyme production by about 16.5-folds from the initial starting conditions. BioMed Central Ltd. 2018 Article PeerReviewed application/pdf en http://eprints.utm.my/79657/1/RoslindaAbdMalek2018_BioprocessOptimizationforPectinaseProduction.pdf El Enshasy, H. A. and Elsayed, E. A. and Suhaimi, N. and Malek, R. A. and Esawy, M. (2018) Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system. BMC Biotechnology, 18 (1). ISSN 1472-6750 http://dx.doi.org/10.1186/s12896-018-0481-7 DOI:10.1186/s12896-018-0481-7 |
| spellingShingle | TP Chemical technology El Enshasy, H. A. Elsayed, E. A. Suhaimi, N. Malek, R. A. Esawy, M. Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system |
| title | Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system |
| title_full | Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system |
| title_fullStr | Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system |
| title_full_unstemmed | Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system |
| title_short | Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system |
| title_sort | bioprocess optimization for pectinase production using aspergillus niger in a submerged cultivation system |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/79657/1/RoslindaAbdMalek2018_BioprocessOptimizationforPectinaseProduction.pdf |
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