Determination of theobromine and caffeine in some malaysian beverages by liquid chromatography-time-of-flight mass spectrometry
Purpose: To determine the concentration of theobromine (TB) and caffeine (CF) in tea and other beverages using liquid chromatography-time of flight-mass spectrometry (LC-TOF-MS). Methods: The extract of caffeine and theobromine from tea and other beverages was filtered by 0.45 μm nylon micro-syringe...
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University of Benin
2018
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| author | Al-Qaim, F. F. Yuzir, A. Mussa, Z. H. |
| author_facet | Al-Qaim, F. F. Yuzir, A. Mussa, Z. H. |
| author_sort | Al-Qaim, F. F. |
| collection | ePrints |
| description | Purpose: To determine the concentration of theobromine (TB) and caffeine (CF) in tea and other beverages using liquid chromatography-time of flight-mass spectrometry (LC-TOF-MS). Methods: The extract of caffeine and theobromine from tea and other beverages was filtered by 0.45 μm nylon micro-syringe and then injected into a LC-ToF-MS system. Theobromine and caffeine were separated using Thermo Scientific C18-column (length 250 mm, width 2.1 mm and diameter 5 μm). Acetonitrile-methanol (ACN – MeOH, 3:1 v/v) was used as mobile phase B, while mobile phase A was 0.1 % FA in DIW. The volume injected was 30 μL at a rate of 0.3 mL/min. Results: Good linearity was obtained in the range of 0.3 – 400 and 0.2 – 200 mg/L for theobromine and caffeine, respectively (regression coefficient (R2) > 0.970). The limits of detection were 0.15 and 0.05 μg/mL for theobromine and caffeine, respectively. The highest concentrations of caffeine and theobromine determined in tea samples were 159.1 and 255.8 mg/L, respectively. Conclusion: Theobromine and caffeine have been successfully analysed in tea, coffee and soft drinks. LC-TOF-MS is an accurate and promising instrument for the determination of the studied compounds in beverages. |
| first_indexed | 2024-03-05T20:21:48Z |
| format | Article |
| id | utm.eprints-79798 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:21:48Z |
| publishDate | 2018 |
| publisher | University of Benin |
| record_format | dspace |
| spelling | utm.eprints-797982019-01-28T06:55:50Z http://eprints.utm.my/79798/ Determination of theobromine and caffeine in some malaysian beverages by liquid chromatography-time-of-flight mass spectrometry Al-Qaim, F. F. Yuzir, A. Mussa, Z. H. T Technology (General) Purpose: To determine the concentration of theobromine (TB) and caffeine (CF) in tea and other beverages using liquid chromatography-time of flight-mass spectrometry (LC-TOF-MS). Methods: The extract of caffeine and theobromine from tea and other beverages was filtered by 0.45 μm nylon micro-syringe and then injected into a LC-ToF-MS system. Theobromine and caffeine were separated using Thermo Scientific C18-column (length 250 mm, width 2.1 mm and diameter 5 μm). Acetonitrile-methanol (ACN – MeOH, 3:1 v/v) was used as mobile phase B, while mobile phase A was 0.1 % FA in DIW. The volume injected was 30 μL at a rate of 0.3 mL/min. Results: Good linearity was obtained in the range of 0.3 – 400 and 0.2 – 200 mg/L for theobromine and caffeine, respectively (regression coefficient (R2) > 0.970). The limits of detection were 0.15 and 0.05 μg/mL for theobromine and caffeine, respectively. The highest concentrations of caffeine and theobromine determined in tea samples were 159.1 and 255.8 mg/L, respectively. Conclusion: Theobromine and caffeine have been successfully analysed in tea, coffee and soft drinks. LC-TOF-MS is an accurate and promising instrument for the determination of the studied compounds in beverages. University of Benin 2018 Article PeerReviewed Al-Qaim, F. F. and Yuzir, A. and Mussa, Z. H. (2018) Determination of theobromine and caffeine in some malaysian beverages by liquid chromatography-time-of-flight mass spectrometry. Tropical Journal of Pharmaceutical Research, 17 (3). pp. 529-535. ISSN 1596-5996 http://dx.doi.org/10.4314/tjpr.v17i3.20 DOI:10.4314/tjpr.v17i3.20 |
| spellingShingle | T Technology (General) Al-Qaim, F. F. Yuzir, A. Mussa, Z. H. Determination of theobromine and caffeine in some malaysian beverages by liquid chromatography-time-of-flight mass spectrometry |
| title | Determination of theobromine and caffeine in some malaysian beverages by liquid chromatography-time-of-flight mass spectrometry |
| title_full | Determination of theobromine and caffeine in some malaysian beverages by liquid chromatography-time-of-flight mass spectrometry |
| title_fullStr | Determination of theobromine and caffeine in some malaysian beverages by liquid chromatography-time-of-flight mass spectrometry |
| title_full_unstemmed | Determination of theobromine and caffeine in some malaysian beverages by liquid chromatography-time-of-flight mass spectrometry |
| title_short | Determination of theobromine and caffeine in some malaysian beverages by liquid chromatography-time-of-flight mass spectrometry |
| title_sort | determination of theobromine and caffeine in some malaysian beverages by liquid chromatography time of flight mass spectrometry |
| topic | T Technology (General) |
| work_keys_str_mv | AT alqaimff determinationoftheobromineandcaffeineinsomemalaysianbeveragesbyliquidchromatographytimeofflightmassspectrometry AT yuzira determinationoftheobromineandcaffeineinsomemalaysianbeveragesbyliquidchromatographytimeofflightmassspectrometry AT mussazh determinationoftheobromineandcaffeineinsomemalaysianbeveragesbyliquidchromatographytimeofflightmassspectrometry |