Purification and characterization of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. G1

Purification and characterization of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. G1

A cyclodextrin glucanotransferase (CGTase) was successively purified by ammonium sulphate precipitation, and affinity chromatography on a-CD (epoxy)-Sepharose 6B column. The specific activity of the CGTase was increased approximately 2200-fold, from 8.43 U/mg protein to 18,866 U/mg protein. SDS-PAGE...

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Bibliographic Details
Main Authors: Ho, Kek Sian, Said, M., Hassan, O., Kamaruddin, K., Ismail, A. F., A. Rahman, R, Nik Mahmood, N. A., Md. Illias, R
Format: Article
Language:English
Published: 2005
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/805/2/HoKekSian2005_PurificationAndCharacterizationOfCyclodextrinGlucanotransferase.pdf

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