Immobilization of phenol degrader pseudomonas sp in repeated batch culture using bioceramic and sponge as support materials

The performance of two types of inert support, namely bioceramic and sponge to immobilize a locally isolated phenol degrader Pseudomonas sp. in a packed column was investigated in repeated batch culture. Prior to this, our study indicated that immobilization had doubled the tolerance limit of the ce...

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Main Authors: Misson, Mailin, Razali, Firdausi
Format: Article
Language:English
English
Published: Penerbit UTM Press 2007
Subjects:
Online Access:http://eprints.utm.my/8096/3/FIrdausiRazali2007_ImmobilizationofPhenolDegraderPseudomonasspinRepeated.pdf
http://eprints.utm.my/8096/4/294
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author Misson, Mailin
Razali, Firdausi
author_facet Misson, Mailin
Razali, Firdausi
author_sort Misson, Mailin
collection ePrints
description The performance of two types of inert support, namely bioceramic and sponge to immobilize a locally isolated phenol degrader Pseudomonas sp. in a packed column was investigated in repeated batch culture. Prior to this, our study indicated that immobilization had doubled the tolerance limit of the cells towards phenol from 1000 ppm (in the suspended culture), to 2000 ppm. For the same volume, the bioceramic managed to trap bacterial cells 1.8 times greater than the sponge did. As a result, it was able to remove 100% of 1000 ppm 600–ml phenol fed at a rate of 2.5 ml/min within 24 hours, and the phenol removal capacity was sustained in the next six consecutive batches. Cells entrapped in sponge however, managed to remove around 90% phenol in five batches. Despite lower performance, at large scales, the use of sponge for cell entrapment offers some merits such as lightness, and easily available at cheaper cost.
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spelling utm.eprints-80962017-11-01T04:17:27Z http://eprints.utm.my/8096/ Immobilization of phenol degrader pseudomonas sp in repeated batch culture using bioceramic and sponge as support materials Misson, Mailin Razali, Firdausi TP Chemical technology The performance of two types of inert support, namely bioceramic and sponge to immobilize a locally isolated phenol degrader Pseudomonas sp. in a packed column was investigated in repeated batch culture. Prior to this, our study indicated that immobilization had doubled the tolerance limit of the cells towards phenol from 1000 ppm (in the suspended culture), to 2000 ppm. For the same volume, the bioceramic managed to trap bacterial cells 1.8 times greater than the sponge did. As a result, it was able to remove 100% of 1000 ppm 600–ml phenol fed at a rate of 2.5 ml/min within 24 hours, and the phenol removal capacity was sustained in the next six consecutive batches. Cells entrapped in sponge however, managed to remove around 90% phenol in five batches. Despite lower performance, at large scales, the use of sponge for cell entrapment offers some merits such as lightness, and easily available at cheaper cost. Penerbit UTM Press 2007-06 Article PeerReviewed application/pdf en http://eprints.utm.my/8096/3/FIrdausiRazali2007_ImmobilizationofPhenolDegraderPseudomonasspinRepeated.pdf text/html en http://eprints.utm.my/8096/4/294 Misson, Mailin and Razali, Firdausi (2007) Immobilization of phenol degrader pseudomonas sp in repeated batch culture using bioceramic and sponge as support materials. Jurnal Teknologi (46F). pp. 51-59. ISSN 2180-3722 DOI:10.11113/jt.v46.304
spellingShingle TP Chemical technology
Misson, Mailin
Razali, Firdausi
Immobilization of phenol degrader pseudomonas sp in repeated batch culture using bioceramic and sponge as support materials
title Immobilization of phenol degrader pseudomonas sp in repeated batch culture using bioceramic and sponge as support materials
title_full Immobilization of phenol degrader pseudomonas sp in repeated batch culture using bioceramic and sponge as support materials
title_fullStr Immobilization of phenol degrader pseudomonas sp in repeated batch culture using bioceramic and sponge as support materials
title_full_unstemmed Immobilization of phenol degrader pseudomonas sp in repeated batch culture using bioceramic and sponge as support materials
title_short Immobilization of phenol degrader pseudomonas sp in repeated batch culture using bioceramic and sponge as support materials
title_sort immobilization of phenol degrader pseudomonas sp in repeated batch culture using bioceramic and sponge as support materials
topic TP Chemical technology
url http://eprints.utm.my/8096/3/FIrdausiRazali2007_ImmobilizationofPhenolDegraderPseudomonasspinRepeated.pdf
http://eprints.utm.my/8096/4/294
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