Formation of chitosan beads and lactic acid production by chitosan-immobilised lactobacillus sp

Chitosan is a biopolymer derived by deacetylation of chitin and has special properties such as hydrophilicity, biocompatibility, and is biodegradable. In this study, chitin was obtained from Tiger prawn waste through fermentation process. The chitin was then deacetylated to form chitosan with variou...

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Main Authors: Rusmin, Ruhaida, Zakaria, Zainoha
Format: Article
Published: SSM International Publication, India 2007
Subjects:
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author Rusmin, Ruhaida
Zakaria, Zainoha
author_facet Rusmin, Ruhaida
Zakaria, Zainoha
author_sort Rusmin, Ruhaida
collection ePrints
description Chitosan is a biopolymer derived by deacetylation of chitin and has special properties such as hydrophilicity, biocompatibility, and is biodegradable. In this study, chitin was obtained from Tiger prawn waste through fermentation process. The chitin was then deacetylated to form chitosan with various degrees of deacetylation. Chitosan beads were prepared by dropping chitosan solution into sodium pyrophosphate which act as gelling agent. Several parameters such as degree of deacetylation (DD), concentration of chitosan solution, pH and concentration of sodium pyrophosphate solution were studied to determine their effect towards the formation and porosity of chitosan beads. High DD, high concentration of chitosan, and high pH of sodium pyrophosphate were found important to facilitate the formation of spherical shaped chitosan beads which is also supported by SEM and IR analyses. The fermentation of glucose by chitosan-immobilised Lactobacillus sp. produced 535 x 10-3 g/L of lactic acid over a period of three days. Upon recycling, chitosan-immobilised cells were able to maintain its shape until the third cycle.
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spelling utm.eprints-82262011-01-22T08:07:57Z http://eprints.utm.my/8226/ Formation of chitosan beads and lactic acid production by chitosan-immobilised lactobacillus sp Rusmin, Ruhaida Zakaria, Zainoha QD Chemistry Chitosan is a biopolymer derived by deacetylation of chitin and has special properties such as hydrophilicity, biocompatibility, and is biodegradable. In this study, chitin was obtained from Tiger prawn waste through fermentation process. The chitin was then deacetylated to form chitosan with various degrees of deacetylation. Chitosan beads were prepared by dropping chitosan solution into sodium pyrophosphate which act as gelling agent. Several parameters such as degree of deacetylation (DD), concentration of chitosan solution, pH and concentration of sodium pyrophosphate solution were studied to determine their effect towards the formation and porosity of chitosan beads. High DD, high concentration of chitosan, and high pH of sodium pyrophosphate were found important to facilitate the formation of spherical shaped chitosan beads which is also supported by SEM and IR analyses. The fermentation of glucose by chitosan-immobilised Lactobacillus sp. produced 535 x 10-3 g/L of lactic acid over a period of three days. Upon recycling, chitosan-immobilised cells were able to maintain its shape until the third cycle. SSM International Publication, India 2007-12 Article PeerReviewed Rusmin, Ruhaida and Zakaria, Zainoha (2007) Formation of chitosan beads and lactic acid production by chitosan-immobilised lactobacillus sp. Asian Chitin Journal, 3 . pp. 23-36. ISSN 0973-3345 http://asianchitinjournal.org/main/?p=145
spellingShingle QD Chemistry
Rusmin, Ruhaida
Zakaria, Zainoha
Formation of chitosan beads and lactic acid production by chitosan-immobilised lactobacillus sp
title Formation of chitosan beads and lactic acid production by chitosan-immobilised lactobacillus sp
title_full Formation of chitosan beads and lactic acid production by chitosan-immobilised lactobacillus sp
title_fullStr Formation of chitosan beads and lactic acid production by chitosan-immobilised lactobacillus sp
title_full_unstemmed Formation of chitosan beads and lactic acid production by chitosan-immobilised lactobacillus sp
title_short Formation of chitosan beads and lactic acid production by chitosan-immobilised lactobacillus sp
title_sort formation of chitosan beads and lactic acid production by chitosan immobilised lactobacillus sp
topic QD Chemistry
work_keys_str_mv AT rusminruhaida formationofchitosanbeadsandlacticacidproductionbychitosanimmobilisedlactobacillussp
AT zakariazainoha formationofchitosanbeadsandlacticacidproductionbychitosanimmobilisedlactobacillussp