Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes
Bacillus lehensis G1 is an alkaliphilic bacterium that is capable of surviving in environments up to pH 11. Secretome related to bacterial acclimation in alkaline environment has been less studied compared to cytoplasmic and membrane proteome. The aim of this study was to gain better understanding o...
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Elsevier B.V.
2018
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| _version_ | 1796863776451985408 |
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| author | How, Lie Ling Rahmat, Zaidah Abu Bakar, Farah Diba Abdul Murad, Abdul Munir Md. Illias, Rosli |
| author_facet | How, Lie Ling Rahmat, Zaidah Abu Bakar, Farah Diba Abdul Murad, Abdul Munir Md. Illias, Rosli |
| author_sort | How, Lie Ling |
| collection | ePrints |
| description | Bacillus lehensis G1 is an alkaliphilic bacterium that is capable of surviving in environments up to pH 11. Secretome related to bacterial acclimation in alkaline environment has been less studied compared to cytoplasmic and membrane proteome. The aim of this study was to gain better understanding of bacterial acclimation to alkaline media through analyzing extracellular proteins of B. lehensis. The pH range for B. lehensis growth was conducted, and two-dimensional electrophoresis and MALDI-TOF/TOF MS analysis were conducted to characterize changes in protein profiling in B. lehensis cultured at pH 8 and pH 11 when compared with those cultured at pH 10 (optimal growth pH). B. lehensis could grow well at pH ranging from 8 to 11 in which the bacteria showed to posses thinner flagella at pH 11. Proteomic analyses demonstrated that five proteins were up-regulated and 13 proteins were down-regulated at pH 8, whereas at pH 11, 14 proteins were up-regulated and 8 were down-regulated. Majority of the differentially expressed proteins were involved in the cell wall, main glycolytic pathways, the metabolism of amino acids and related molecules and some proteins of unknown function. A total of 40 differentially expressed protein spots corresponding to 33 proteins were identified; including GlcNAc-binding protein A, chitinase, endopeptidase lytE, flagellar hook-associated proteins and enolase. These proteins may play important roles in acclimation to alkaline media via reallocation of cell wall structure and changes to cell surface glycolytic enzymes, amino acid metabolism, flagellar hook-associated proteins and chaperones to sustain life under pH-stressed conditions. |
| first_indexed | 2024-03-05T20:31:49Z |
| format | Article |
| id | utm.eprints-84091 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:31:49Z |
| publishDate | 2018 |
| publisher | Elsevier B.V. |
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| spelling | utm.eprints-840912019-12-16T01:53:38Z http://eprints.utm.my/84091/ Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes How, Lie Ling Rahmat, Zaidah Abu Bakar, Farah Diba Abdul Murad, Abdul Munir Md. Illias, Rosli TP Chemical technology Bacillus lehensis G1 is an alkaliphilic bacterium that is capable of surviving in environments up to pH 11. Secretome related to bacterial acclimation in alkaline environment has been less studied compared to cytoplasmic and membrane proteome. The aim of this study was to gain better understanding of bacterial acclimation to alkaline media through analyzing extracellular proteins of B. lehensis. The pH range for B. lehensis growth was conducted, and two-dimensional electrophoresis and MALDI-TOF/TOF MS analysis were conducted to characterize changes in protein profiling in B. lehensis cultured at pH 8 and pH 11 when compared with those cultured at pH 10 (optimal growth pH). B. lehensis could grow well at pH ranging from 8 to 11 in which the bacteria showed to posses thinner flagella at pH 11. Proteomic analyses demonstrated that five proteins were up-regulated and 13 proteins were down-regulated at pH 8, whereas at pH 11, 14 proteins were up-regulated and 8 were down-regulated. Majority of the differentially expressed proteins were involved in the cell wall, main glycolytic pathways, the metabolism of amino acids and related molecules and some proteins of unknown function. A total of 40 differentially expressed protein spots corresponding to 33 proteins were identified; including GlcNAc-binding protein A, chitinase, endopeptidase lytE, flagellar hook-associated proteins and enolase. These proteins may play important roles in acclimation to alkaline media via reallocation of cell wall structure and changes to cell surface glycolytic enzymes, amino acid metabolism, flagellar hook-associated proteins and chaperones to sustain life under pH-stressed conditions. Elsevier B.V. 2018 Article PeerReviewed How, Lie Ling and Rahmat, Zaidah and Abu Bakar, Farah Diba and Abdul Murad, Abdul Munir and Md. Illias, Rosli (2018) Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes. Microbiological Research, 215 . pp. 46-54. ISSN 0944-5013 https://doi.org/10.1016/j.micres.2018.06.006 |
| spellingShingle | TP Chemical technology How, Lie Ling Rahmat, Zaidah Abu Bakar, Farah Diba Abdul Murad, Abdul Munir Md. Illias, Rosli Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes |
| title | Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes |
| title_full | Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes |
| title_fullStr | Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes |
| title_full_unstemmed | Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes |
| title_short | Secretome analysis of alkaliphilic bacterium Bacillus lehensis G1 in response to pH changes |
| title_sort | secretome analysis of alkaliphilic bacterium bacillus lehensis g1 in response to ph changes |
| topic | TP Chemical technology |
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