Insight into the Rhizomucor miehei lipase supported on chitosan-chitin nanowhiskers assisted esterification of eugenol to eugenyl benzoate
To overcome drawbacks in the conventional chemical route to synthesize eugenyl benzoate, immobilized Rhizomucor miehei lipase (RML) as the biocatalyst was proposed. The RML conjugated to a hybrid support consisting of biopolymers, chitosan (CS) and chitin nanowhiskers (CNWs). 1-ethyl-3-[3-dimethylam...
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| Format: | Article |
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Elsevier B.V.
2018
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| author | Abd. Manan, Fatin Myra Attan, Nursyafreena Zakaria, Zainoha Mahat, Naji Arafat Abdul Wahab, Roswanira |
| author_facet | Abd. Manan, Fatin Myra Attan, Nursyafreena Zakaria, Zainoha Mahat, Naji Arafat Abdul Wahab, Roswanira |
| author_sort | Abd. Manan, Fatin Myra |
| collection | ePrints |
| description | To overcome drawbacks in the conventional chemical route to synthesize eugenyl benzoate, immobilized Rhizomucor miehei lipase (RML) as the biocatalyst was proposed. The RML conjugated to a hybrid support consisting of biopolymers, chitosan (CS) and chitin nanowhiskers (CNWs). 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDAC) was used as the crosslinker to bind the lipase. Immobilization of RML was the highest on crosslinked CS/CNWs which gave a protein loading of ∼8.12 mg/g, corresponding to specific and residual activity of 537 U/g and 137%, respectively. Fourier transform infrared spectroscopy, thermogravimetric analysis-differential thermogravimetry, field emission scanning electron and atomic force microscopy of RML-CS/CNWs revealed that RML was successfully attached to the surface of crosslinked CS/CNWs. Under an optimized condition, the highest yield of eugenyl benzoate (56.3%) was attained after 5 h using 3 mg/mL of RML-CS/CNWs with molar ratio of eugenol: benzoic acid of 3:1, as compared to only 47.3% for the free RML. Analyses of FTIR and NMR on purified eugenyl benzoate affirmed that the ester was successfully produced in the enzymatic esterification. Therefore, the use of the RML-CS/CNWs biocatalysts appears promising to afford good yields of eugenyl benzoate within a relatively shorter reaction time. |
| first_indexed | 2024-03-05T20:32:15Z |
| format | Article |
| id | utm.eprints-84238 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:32:15Z |
| publishDate | 2018 |
| publisher | Elsevier B.V. |
| record_format | dspace |
| spelling | utm.eprints-842382019-12-16T03:15:44Z http://eprints.utm.my/84238/ Insight into the Rhizomucor miehei lipase supported on chitosan-chitin nanowhiskers assisted esterification of eugenol to eugenyl benzoate Abd. Manan, Fatin Myra Attan, Nursyafreena Zakaria, Zainoha Mahat, Naji Arafat Abdul Wahab, Roswanira QD Chemistry To overcome drawbacks in the conventional chemical route to synthesize eugenyl benzoate, immobilized Rhizomucor miehei lipase (RML) as the biocatalyst was proposed. The RML conjugated to a hybrid support consisting of biopolymers, chitosan (CS) and chitin nanowhiskers (CNWs). 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDAC) was used as the crosslinker to bind the lipase. Immobilization of RML was the highest on crosslinked CS/CNWs which gave a protein loading of ∼8.12 mg/g, corresponding to specific and residual activity of 537 U/g and 137%, respectively. Fourier transform infrared spectroscopy, thermogravimetric analysis-differential thermogravimetry, field emission scanning electron and atomic force microscopy of RML-CS/CNWs revealed that RML was successfully attached to the surface of crosslinked CS/CNWs. Under an optimized condition, the highest yield of eugenyl benzoate (56.3%) was attained after 5 h using 3 mg/mL of RML-CS/CNWs with molar ratio of eugenol: benzoic acid of 3:1, as compared to only 47.3% for the free RML. Analyses of FTIR and NMR on purified eugenyl benzoate affirmed that the ester was successfully produced in the enzymatic esterification. Therefore, the use of the RML-CS/CNWs biocatalysts appears promising to afford good yields of eugenyl benzoate within a relatively shorter reaction time. Elsevier B.V. 2018-08 Article PeerReviewed Abd. Manan, Fatin Myra and Attan, Nursyafreena and Zakaria, Zainoha and Mahat, Naji Arafat and Abdul Wahab, Roswanira (2018) Insight into the Rhizomucor miehei lipase supported on chitosan-chitin nanowhiskers assisted esterification of eugenol to eugenyl benzoate. Journal of Biotechnology, 280 . pp. 19-30. ISSN 0168-1656 http://dx.doi.org/10.1016/j.jbiotec.2018.05.015 |
| spellingShingle | QD Chemistry Abd. Manan, Fatin Myra Attan, Nursyafreena Zakaria, Zainoha Mahat, Naji Arafat Abdul Wahab, Roswanira Insight into the Rhizomucor miehei lipase supported on chitosan-chitin nanowhiskers assisted esterification of eugenol to eugenyl benzoate |
| title | Insight into the Rhizomucor miehei lipase supported on chitosan-chitin nanowhiskers assisted esterification of eugenol to eugenyl benzoate |
| title_full | Insight into the Rhizomucor miehei lipase supported on chitosan-chitin nanowhiskers assisted esterification of eugenol to eugenyl benzoate |
| title_fullStr | Insight into the Rhizomucor miehei lipase supported on chitosan-chitin nanowhiskers assisted esterification of eugenol to eugenyl benzoate |
| title_full_unstemmed | Insight into the Rhizomucor miehei lipase supported on chitosan-chitin nanowhiskers assisted esterification of eugenol to eugenyl benzoate |
| title_short | Insight into the Rhizomucor miehei lipase supported on chitosan-chitin nanowhiskers assisted esterification of eugenol to eugenyl benzoate |
| title_sort | insight into the rhizomucor miehei lipase supported on chitosan chitin nanowhiskers assisted esterification of eugenol to eugenyl benzoate |
| topic | QD Chemistry |
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