Optimization and characterization of exo-polygalacturonase by Aspergillus niger cultured via solid state fermentation
Polygalacturonases represent an important member of pectinases group of enzymes with immense industrial applications. The activity of exo-polygalacturonase produced by Aspergillus niger was studied in solid state fermentation (SSF) using Nephrolepis biserrata leaves as substrate. Central composite d...
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| Format: | Article |
| Language: | English |
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Penerbit UTM Press
2018
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| Online Access: | http://eprints.utm.my/84717/1/RoshanidaARahman2018_OptimizationandCharacterizationofExoPolygalacturonase.pdf |
| _version_ | 1796863902719410176 |
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| author | Pagarra, Halifah A. Rahman, Roshanida Wan Azelee, Nur Izyan Md. Illias, Rosli |
| author_facet | Pagarra, Halifah A. Rahman, Roshanida Wan Azelee, Nur Izyan Md. Illias, Rosli |
| author_sort | Pagarra, Halifah |
| collection | ePrints |
| description | Polygalacturonases represent an important member of pectinases group of enzymes with immense industrial applications. The activity of exo-polygalacturonase produced by Aspergillus niger was studied in solid state fermentation (SSF) using Nephrolepis biserrata leaves as substrate. Central composite design (CCD) was used to optimize four significant variables resulted from the screening process that has been initially analyzed for the production of exo-polygalacturonase which are incubation time, temperature, concentration of pectin and moisture content. The optimum exo-polygalacturonase production obtained was 54.64 U/g at 120 hours of incubation time, temperature at 340C, 5.0 g/L of pectin concentration and 75.26% of moisture content. For partial characterization of exo-polygalacturonase, the optimum temperature and pH were obtained at 50°C and pH 4.0, respectively. SDS-PAGE analysis showed that molecular weight of exo-polygalacturonase were 35 and 71 kDa. This study has revealed a significant production of exo-polygalacturonase by A. niger under SSF using cheap and easily available substrate and thus could found immense potential application in industrial sectors and biotechnology. |
| first_indexed | 2024-03-05T20:33:41Z |
| format | Article |
| id | utm.eprints-84717 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T20:33:41Z |
| publishDate | 2018 |
| publisher | Penerbit UTM Press |
| record_format | dspace |
| spelling | utm.eprints-847172020-02-29T12:29:13Z http://eprints.utm.my/84717/ Optimization and characterization of exo-polygalacturonase by Aspergillus niger cultured via solid state fermentation Pagarra, Halifah A. Rahman, Roshanida Wan Azelee, Nur Izyan Md. Illias, Rosli TP Chemical technology Polygalacturonases represent an important member of pectinases group of enzymes with immense industrial applications. The activity of exo-polygalacturonase produced by Aspergillus niger was studied in solid state fermentation (SSF) using Nephrolepis biserrata leaves as substrate. Central composite design (CCD) was used to optimize four significant variables resulted from the screening process that has been initially analyzed for the production of exo-polygalacturonase which are incubation time, temperature, concentration of pectin and moisture content. The optimum exo-polygalacturonase production obtained was 54.64 U/g at 120 hours of incubation time, temperature at 340C, 5.0 g/L of pectin concentration and 75.26% of moisture content. For partial characterization of exo-polygalacturonase, the optimum temperature and pH were obtained at 50°C and pH 4.0, respectively. SDS-PAGE analysis showed that molecular weight of exo-polygalacturonase were 35 and 71 kDa. This study has revealed a significant production of exo-polygalacturonase by A. niger under SSF using cheap and easily available substrate and thus could found immense potential application in industrial sectors and biotechnology. Penerbit UTM Press 2018 Article PeerReviewed application/pdf en http://eprints.utm.my/84717/1/RoshanidaARahman2018_OptimizationandCharacterizationofExoPolygalacturonase.pdf Pagarra, Halifah and A. Rahman, Roshanida and Wan Azelee, Nur Izyan and Md. Illias, Rosli (2018) Optimization and characterization of exo-polygalacturonase by Aspergillus niger cultured via solid state fermentation. Jurnal Teknologi, 81 (1). pp. 59-67. ISSN 2180–3722 https://dx.doi.org/10.11113/jt.v81.12222 DOI:10.11113/jt.v81.12222 |
| spellingShingle | TP Chemical technology Pagarra, Halifah A. Rahman, Roshanida Wan Azelee, Nur Izyan Md. Illias, Rosli Optimization and characterization of exo-polygalacturonase by Aspergillus niger cultured via solid state fermentation |
| title | Optimization and characterization of exo-polygalacturonase by Aspergillus niger cultured via solid state fermentation |
| title_full | Optimization and characterization of exo-polygalacturonase by Aspergillus niger cultured via solid state fermentation |
| title_fullStr | Optimization and characterization of exo-polygalacturonase by Aspergillus niger cultured via solid state fermentation |
| title_full_unstemmed | Optimization and characterization of exo-polygalacturonase by Aspergillus niger cultured via solid state fermentation |
| title_short | Optimization and characterization of exo-polygalacturonase by Aspergillus niger cultured via solid state fermentation |
| title_sort | optimization and characterization of exo polygalacturonase by aspergillus niger cultured via solid state fermentation |
| topic | TP Chemical technology |
| url | http://eprints.utm.my/84717/1/RoshanidaARahman2018_OptimizationandCharacterizationofExoPolygalacturonase.pdf |
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