Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice
Glaciozyma antarctica PI12, is a psychrophilic yeast isolated from Antarctic sea. In this work, Expressed Sequence Tags (EST) from cells exposed to three different temperatures; 15 °C, 0 °C and −12 °C were generated to identify genes associated with cold adaptation. A total of 5376 clones from each...
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| Format: | Article |
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Elsevier Ltd
2018
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| _version_ | 1796863961753190400 |
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| author | Bharudin, Izwan Abu Bakar, Mohd. Faizal Hashim, Noor Haza Fazlin Mat Isa, Mohd. Noor Alias, Halimah Raih, Mohd. Firdaus Md. Illias, Rosli Najimudin, N. Mahadi, Nor Muhammad Abu Bakar, Farah Diba Abdul Murad, Abdul Munir |
| author_facet | Bharudin, Izwan Abu Bakar, Mohd. Faizal Hashim, Noor Haza Fazlin Mat Isa, Mohd. Noor Alias, Halimah Raih, Mohd. Firdaus Md. Illias, Rosli Najimudin, N. Mahadi, Nor Muhammad Abu Bakar, Farah Diba Abdul Murad, Abdul Munir |
| author_sort | Bharudin, Izwan |
| collection | ePrints |
| description | Glaciozyma antarctica PI12, is a psychrophilic yeast isolated from Antarctic sea. In this work, Expressed Sequence Tags (EST) from cells exposed to three different temperatures; 15 °C, 0 °C and −12 °C were generated to identify genes associated with cold adaptation. A total of 5376 clones from each library were randomly picked and sequenced. Comparative analyses from the resulting ESTs in each condition identified several groups of genes required for cold adaptation. Additionally, 319 unique transcripts that encoded uncharacterised functions were identified in the −12 °C library and are currently unique to G. antarctica. Gene expression analysis using RT-qPCR revealed two of the unknown genes to be up-regulated at −12 °C compared to 0 °C and 15 °C. These findings further contribute to the collective knowledge into G. antarctica cold adaptation and as a resource for understanding the ecological and physiological tolerance of psychrophilic microbes in general. |
| first_indexed | 2024-03-05T20:34:34Z |
| format | Article |
| id | utm.eprints-85018 |
| institution | Universiti Teknologi Malaysia - ePrints |
| last_indexed | 2024-03-05T20:34:34Z |
| publishDate | 2018 |
| publisher | Elsevier Ltd |
| record_format | dspace |
| spelling | utm.eprints-850182020-02-29T13:19:58Z http://eprints.utm.my/85018/ Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice Bharudin, Izwan Abu Bakar, Mohd. Faizal Hashim, Noor Haza Fazlin Mat Isa, Mohd. Noor Alias, Halimah Raih, Mohd. Firdaus Md. Illias, Rosli Najimudin, N. Mahadi, Nor Muhammad Abu Bakar, Farah Diba Abdul Murad, Abdul Munir Q Science (General) Glaciozyma antarctica PI12, is a psychrophilic yeast isolated from Antarctic sea. In this work, Expressed Sequence Tags (EST) from cells exposed to three different temperatures; 15 °C, 0 °C and −12 °C were generated to identify genes associated with cold adaptation. A total of 5376 clones from each library were randomly picked and sequenced. Comparative analyses from the resulting ESTs in each condition identified several groups of genes required for cold adaptation. Additionally, 319 unique transcripts that encoded uncharacterised functions were identified in the −12 °C library and are currently unique to G. antarctica. Gene expression analysis using RT-qPCR revealed two of the unknown genes to be up-regulated at −12 °C compared to 0 °C and 15 °C. These findings further contribute to the collective knowledge into G. antarctica cold adaptation and as a resource for understanding the ecological and physiological tolerance of psychrophilic microbes in general. Elsevier Ltd 2018-06 Article PeerReviewed Bharudin, Izwan and Abu Bakar, Mohd. Faizal and Hashim, Noor Haza Fazlin and Mat Isa, Mohd. Noor and Alias, Halimah and Raih, Mohd. Firdaus and Md. Illias, Rosli and Najimudin, N. and Mahadi, Nor Muhammad and Abu Bakar, Farah Diba and Abdul Murad, Abdul Munir (2018) Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice. Marine Environmental Research, 137 . pp. 169-176. ISSN 0141-1136 http://dx.doi.org/10.1016/j.marenvres.2018.03.007 |
| spellingShingle | Q Science (General) Bharudin, Izwan Abu Bakar, Mohd. Faizal Hashim, Noor Haza Fazlin Mat Isa, Mohd. Noor Alias, Halimah Raih, Mohd. Firdaus Md. Illias, Rosli Najimudin, N. Mahadi, Nor Muhammad Abu Bakar, Farah Diba Abdul Murad, Abdul Munir Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice |
| title | Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice |
| title_full | Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice |
| title_fullStr | Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice |
| title_full_unstemmed | Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice |
| title_short | Unravelling the adaptation strategies employed by Glaciozyma antarctica PI12 on Antarctic sea ice |
| title_sort | unravelling the adaptation strategies employed by glaciozyma antarctica pi12 on antarctic sea ice |
| topic | Q Science (General) |
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