Synthesis and optimization of nano-sized bacterial-based violacein pigment using response surface methodology
Violacein from Chromobacterium violaceum has raised the enthusiasm of researchers in conducting comprehensive studies on these pigments due to their diverse biological activities including antibacterial and antioxidant properties. However, a limitation related with the solubility of the violacein pi...
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| Format: | Article |
| Language: | English |
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Penerbit UTM Press
2019
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| Online Access: | http://eprints.utm.my/85079/1/SitiAminahSetu2019_SynthesisandOptimizationofNano-SizedBacterial.pdf |
| _version_ | 1796863973624119296 |
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| author | Mohd. Yatim, Haryani Arul Aruldass, Claira Mohd. Hamzah, Mohd. Amir Asyraf Ahmad, Wan Azlina Setu, Siti Aminah |
| author_facet | Mohd. Yatim, Haryani Arul Aruldass, Claira Mohd. Hamzah, Mohd. Amir Asyraf Ahmad, Wan Azlina Setu, Siti Aminah |
| author_sort | Mohd. Yatim, Haryani |
| collection | ePrints |
| description | Violacein from Chromobacterium violaceum has raised the enthusiasm of researchers in conducting comprehensive studies on these pigments due to their diverse biological activities including antibacterial and antioxidant properties. However, a limitation related with the solubility of the violacein pigment, by which it is commonly dissolved in toxic solvents such as dimethyl sulfoxide and methanol instead of being soluble in biological fluids and water. Hence, this study provides a method to synthesis the violacein pigment in nanoscale through an encapsulation technique using chitosan-tripolyphosphate (Cs-TPP) nanoparticles. The synthesis of nanoparticles in this study involved ionic gelation between chitosan and tripolyphosphate (TPP), in which several parameters were taken into consideration in order to control the size and dispersion stability of the violacein pigment in the suspension. Preparation parameters, including the concentration of chitosan, TPP and pigment as well as the mass ratio of chitosan to TPP, were optimized using Response Surface Methodology (RSM). Minimum particle size of 149.0 nm with zeta potential of +23.40 mV was obtained at the optimal formulations of 2.33 mg/mL of chitosan, 1.5 mg/mL of TPP, and 1 ppm of violacein pigment and at mass ratio of chitosan:TPP of 7:1. This nano-sized violacein pigment is expected to be applied as safe additive, colorant, and therapeutic agents. Meanwhile, RSM in the study could provide the optimal formulations for producing stable nano-sized violacein pigment. |
| first_indexed | 2024-03-05T20:34:49Z |
| format | Article |
| id | utm.eprints-85079 |
| institution | Universiti Teknologi Malaysia - ePrints |
| language | English |
| last_indexed | 2024-03-05T20:34:49Z |
| publishDate | 2019 |
| publisher | Penerbit UTM Press |
| record_format | dspace |
| spelling | utm.eprints-850792020-03-04T01:39:10Z http://eprints.utm.my/85079/ Synthesis and optimization of nano-sized bacterial-based violacein pigment using response surface methodology Mohd. Yatim, Haryani Arul Aruldass, Claira Mohd. Hamzah, Mohd. Amir Asyraf Ahmad, Wan Azlina Setu, Siti Aminah QD Chemistry Violacein from Chromobacterium violaceum has raised the enthusiasm of researchers in conducting comprehensive studies on these pigments due to their diverse biological activities including antibacterial and antioxidant properties. However, a limitation related with the solubility of the violacein pigment, by which it is commonly dissolved in toxic solvents such as dimethyl sulfoxide and methanol instead of being soluble in biological fluids and water. Hence, this study provides a method to synthesis the violacein pigment in nanoscale through an encapsulation technique using chitosan-tripolyphosphate (Cs-TPP) nanoparticles. The synthesis of nanoparticles in this study involved ionic gelation between chitosan and tripolyphosphate (TPP), in which several parameters were taken into consideration in order to control the size and dispersion stability of the violacein pigment in the suspension. Preparation parameters, including the concentration of chitosan, TPP and pigment as well as the mass ratio of chitosan to TPP, were optimized using Response Surface Methodology (RSM). Minimum particle size of 149.0 nm with zeta potential of +23.40 mV was obtained at the optimal formulations of 2.33 mg/mL of chitosan, 1.5 mg/mL of TPP, and 1 ppm of violacein pigment and at mass ratio of chitosan:TPP of 7:1. This nano-sized violacein pigment is expected to be applied as safe additive, colorant, and therapeutic agents. Meanwhile, RSM in the study could provide the optimal formulations for producing stable nano-sized violacein pigment. Penerbit UTM Press 2019 Article PeerReviewed application/pdf en http://eprints.utm.my/85079/1/SitiAminahSetu2019_SynthesisandOptimizationofNano-SizedBacterial.pdf Mohd. Yatim, Haryani and Arul Aruldass, Claira and Mohd. Hamzah, Mohd. Amir Asyraf and Ahmad, Wan Azlina and Setu, Siti Aminah (2019) Synthesis and optimization of nano-sized bacterial-based violacein pigment using response surface methodology. Malaysian Journal of Fundamental and Applied Sciences, 15 (6). pp. 818-824. ISSN 2289-5981 https://mjfas.utm.my/index.php/mjfas/article/download/1271/pdf |
| spellingShingle | QD Chemistry Mohd. Yatim, Haryani Arul Aruldass, Claira Mohd. Hamzah, Mohd. Amir Asyraf Ahmad, Wan Azlina Setu, Siti Aminah Synthesis and optimization of nano-sized bacterial-based violacein pigment using response surface methodology |
| title | Synthesis and optimization of nano-sized bacterial-based violacein pigment using response surface methodology |
| title_full | Synthesis and optimization of nano-sized bacterial-based violacein pigment using response surface methodology |
| title_fullStr | Synthesis and optimization of nano-sized bacterial-based violacein pigment using response surface methodology |
| title_full_unstemmed | Synthesis and optimization of nano-sized bacterial-based violacein pigment using response surface methodology |
| title_short | Synthesis and optimization of nano-sized bacterial-based violacein pigment using response surface methodology |
| title_sort | synthesis and optimization of nano sized bacterial based violacein pigment using response surface methodology |
| topic | QD Chemistry |
| url | http://eprints.utm.my/85079/1/SitiAminahSetu2019_SynthesisandOptimizationofNano-SizedBacterial.pdf |
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